LOCUS       V004451                 5908 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004451
VERSION     V004451
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5908)
  AUTHORS   Bair CR, Kotha Lakshmi Narayan P, Kajon AE.
  TITLE     The Adenovirus Tripartite Leader Sequence Enables Efficient Ectopic
            Expression of Human Adenovirus E3 CR1 Transcripts
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5908)
  AUTHORS   Bair CR, Kotha Lakshmi Narayan P, Lamson DM, Kajon AE.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-AUG-2016) Infectous Disease Program, Lovelace
            Respiratory Research Institute, 2425 Ridgecrest Drive SE,
            Albuquerque, NM 87108, USA
REFERENCE   3  (bases 1 to 5908)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5908)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Geneious v. 8.1.7
            Sequencing Technology :: Illumina; Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (19-AUG-2016) Infectous Disease Program, Lovelace Respiratory
            Research Institute, 2425 Ridgecrest Drive SE, Albuquerque, NM 87108,
            USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5908
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..65
                     /label="multiple cloning sites"
                     /note="multiple cloning sites"
     polyA_signal    95..229
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     CDS             complement(630..1718)
                     /gene="ADA"
                     /label="Adenosine deaminase"
                     /note="Adenosine deaminase from Homo sapiens. Accession#:
                     P00813"
     promoter        complement(1799..2115)
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     misc_feature    2138..2371
                     /label="VARNA"
                     /note="VARNA"
     rep_origin      complement(2623..3211)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3385..4242)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4243..4347)
                     /label="AmpR promoter"
     primer_bind     4821..4837
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        complement(4847..5176)
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     regulatory      5204..5504
                     /label="human adenovirus type 2 major late promoter"
                     /note="human adenovirus type 2 major late promoter"
                     /regulatory_class="promoter"
     misc_feature    5505..5636
                     /note="leader 2 and leader 3 from the human adenovirus type
                     2 tripartite leader sequence"
     intron          5645..5874
                     /label="chimeric intron"
                     /note="chimera between introns from adenovirus and
                     immunoglobulin heavy chain genes"
ORIGIN
        1 gaattcacgc gtggtacctc tagagtcgac ccgggcggcc gcttcccttt agtatcgatg
       61 aattccgtcg actctagagc cgtcgactct agagaacttg tttattgcag cttataatgg
      121 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
      181 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatcc ccggccaacg
      241 gtctggtgac ccggctgcga gagctcggtg tacctgagac gcgagtaagc ccttgagtca
      301 aagacgtagt cgttgcaagt ccgcaccagg tactgatatc ccaccaaaaa gtgcggcggc
      361 ggctggcggt agaggggcca gcgtagggtg gccggggctc cgggggcgag gtcttccaac
      421 ataaggcgat gatatccgta gatgtacctg gacatccagg tgatgccggc ggcggtggtg
      481 gaggcgcgcg gaaagtcgcg gacgcggttc cagatgttgc gcagcggcaa aaagtgctcc
      541 atggtcggga cgctctggcc ggtgaggcgt gcgcagtcgt tgacgctcta gactccacag
      601 ggtgaaggct tggaggagtg gcgtcttcag aggttctgcc ctgcagaggc tgaaggtggc
      661 atcccatagg ctttatagag caggtcgaga agctccctct tttcatcttc tgggaggaaa
      721 ctagatttgg ccgcattgat gttcagcctt ttaaactcct cttcagtaaa gcccatgtcc
      781 cgtttggtca tctggtaatc agtgtccagg gtggacttga agatgagcgg gtcatctgtg
      841 ttgagcgagt agttagcctg gtcatttttg agccgaatga ctgcatgctc cgtgtccggc
      901 ttccaggcac cagtgaggta gctggaccag gggcagatct cgaagtgcat gttttcctgc
      961 cgcagcctgt tataaagggc ctggtcttcc agggtgtggt agccgtgtcc cagccgctct
     1021 gtcttgagta tgtccacagc ctcttttact acttcggccg agcccacctc cccggcgtgg
     1081 acagtacggt gaatgccgct cttcacagcc tcctggtagg cctggacatg tccaggcaag
     1141 aggctgcttc ctgggatggt ctcatctcca gccaggtcaa tggctaccac ggtctgctgc
     1201 tggtacttct tacacagctc caccaccttg ggggaccagt tgggctggtg gcgcatgcag
     1261 cacaggatgg accgggcctt gaccccgaag tctcgctccc cctcctgcag gccctggccc
     1321 actagggcca ccacctcgtc tggggtgagg tccccttcag cctggttcca ggggattggc
     1381 tccactttgg agttggccag caggtgcgga ctgtaccgca cctccacata caccacgccc
     1441 tctttagcct tcatctctac aaactcatag gcgatccttt tgatagcctc ccggcagccc
     1501 gcgatagcag gcatgtagta gtcaaatttg gccaggaagt ctggaagggt gagcggcttg
     1561 tccatgccaa tgacgttcag cagcccctct gctgtgttag ctgggagggc gatccctctc
     1621 ctcctgccat agtataagat ggtttcaggc ttgatggatc cgtctaggtg gacatgcagt
     1681 tctactttgg gcttgtcgaa ggcgggcgtc tgggccatgg tgccctcgtg cgccccggcg
     1741 ctgctccctc cgccgccgct cggtgggtct ctgccggaat taattcaagc ttgagctttt
     1801 tgcaaaagcc taggcctcca aaaaagcctc ctcactactt ctggaatagc tcagaggccg
     1861 aggcggcctc ggcctctgca taaataaaaa aaattagtca gccatggggc ggagaatggg
     1921 cggaactggg cggagttagg ggcgggatgg gcggagttag gggcgggact atggttgctg
     1981 actaattgag atgcatgctt tgcatacttc tgcctgctgg ggagcctggg gactttccac
     2041 acctggttgc tgactaattg agatgcatgc tttgcatact tctgcctgct ggggagcctg
     2101 gggactttcc acaccctaac tgacacacat tccacagcta gaccgtgcaa aaggagagcc
     2161 tgtaagcggg cactcttccg tggtctggtg gataaattcg caagggtatc atggcggacg
     2221 accggggttc gaaccccgga tccggccgtc cgccgtgatc catgcggtta ccgcccgcgt
     2281 gtcgaaccca ggtgtgcgac gtcagacaac gggggagcgc tccttttggc ttccttccag
     2341 gcgcggcggc tgctgcgcta gcttttttgg cgagctcgaa ttaattctgc attaatgaat
     2401 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     2461 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     2521 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     2581 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     2641 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     2701 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     2761 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     2821 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     2881 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     2941 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     3001 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     3061 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     3121 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     3181 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     3241 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     3301 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     3361 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     3421 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     3481 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     3541 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     3601 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     3661 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     3721 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     3781 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     3841 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     3901 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     3961 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     4021 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     4081 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     4141 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     4201 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     4261 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     4321 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
     4381 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
     4441 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
     4501 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
     4561 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt
     4621 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg
     4681 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
     4741 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
     4801 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctttttg caaaagccta
     4861 ggcctccaaa aaagcctcct cactacttct ggaatagctc agaggccgag gcggcctcgg
     4921 cctctgcata aataaaaaaa attagtcagc catggggcgg agaatgggcg gaactgggcg
     4981 gagttagggg cgggatgggc ggagttaggg gcgggactat ggttgctgac taattgagat
     5041 gcatgctttg catacttctg cctgctgggg agcctgggga ctttccacac ctggttgctg
     5101 actaattgag atgcatgctt tgcatacttc tgcctgctgg ggagcctggg gactttccac
     5161 accctaactg acacacattc cacaggatcc ggtcgcgcga atttcgagcg gtgttccgcg
     5221 gtcctcctcg tatagaaact cggaccactc tgagacgaag gctcgcgtcc aggccagcac
     5281 gaaggaggct aagtgggagg ggtagcggtc gttgtccact agggggtcca ctcgctccag
     5341 ggtgtgaaga cacatgtcgc cctcttcggc atcaaggaag gtgattggtt tataggtgta
     5401 ggccacgtga ccgggtgttc ctgaaggggg gctataaaag ggggtggggg cgcgttcgtc
     5461 ctcactctct tccgcatcgc tgtctgcgag ggccagctgt tgggctcgcg gttgaggaca
     5521 aactcttcgc ggtctttcca gtactcttgg atcggaaacc cgtcggcctc cgaacggtac
     5581 tccgccaccg agggacctga gcgagtccgc atcgaccgga tcggaaaacc tctcgactgt
     5641 tggggtgagt actccctctc aaaagcgggc atgacttctg cgctaagatt gtcagtttcc
     5701 aaaaacgagg aggatttgat attcacctgg cccgcggtga tgcctttgag ggtggccgcg
     5761 tccatctggt cagaaaagac aatctttttg ttgtcaagct tgaggtgtgg caggcttgag
     5821 atctggccat acacttgagt gacaatgaca tccactttgc ctttctctcc acaggtgtcc
     5881 actcccaggt ccaactgcag gcgagcct
//
