pMSW105 vector (V004454)

LOCUS       40924_32420        7120 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pMSW105, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7120)
  AUTHORS   Wecker MS, Beaton S, Chado R, Ghirardi ML.
  TITLE     Development of a Rhodobacter capsulatus self-reporting model system 
            for optimizing light-dependent, [FeFe]-hydrogenase-driven H 
            production
  JOURNAL   Biotechnol. Bioeng. (2016) In press
  PUBMED    27531314
REFERENCE   2  (bases 1 to 7120)
  AUTHORS   Wecker MS.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAY-2016) Molecular Biology, GeneBiologics, 1047 
            Cherryvale Road, Boulder, CO 80303, USA
REFERENCE   3  (bases 1 to 7120)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7120)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol.
            Bioeng. (2016) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (26-MAY-2016) Molecular Biology, GeneBiologics, 1047 Cherryvale 
            Road, Boulder, CO 80303, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7120
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(264..794)
                     /label=GmR
                     /note="gentamycin acetyltransferase"
     promoter        complement(983..1011)
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    1281..1302
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1317..1347
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1355..1371
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1379..1395
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1416..1434
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      1460..2094
                     /note="fru promoter; derived from Rhodobacter capsulatus"
                     /regulatory_class="promoter"
     CDS             3844..3867
                     /label=Strep-Tag II
                     /note="peptide that binds Strep-Tactin(R), an engineered 
                     form
                     of streptavidin"
     promoter        complement(3915..3933)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3943..3959)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(4669..5328)
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
     rep_origin      complement(5329..6098)
                     /direction=LEFT
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"