Basic Vector Information
- Vector Name:
- pMSW105
- Antibiotic Resistance:
- Gentamycin
- Length:
- 7120 bp
- Type:
- Cloning vector
- Replication origin:
- pBBR1 oriV
- Source/Author:
- Wecker MS, Beaton S, Chado R, Ghirardi ML.
- Promoter:
- Pc
pMSW105 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pMSW105 vector Sequence
LOCUS 40924_32420 7120 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector pMSW105, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7120)
AUTHORS Wecker MS, Beaton S, Chado R, Ghirardi ML.
TITLE Development of a Rhodobacter capsulatus self-reporting model system
for optimizing light-dependent, [FeFe]-hydrogenase-driven H
production
JOURNAL Biotechnol. Bioeng. (2016) In press
PUBMED 27531314
REFERENCE 2 (bases 1 to 7120)
AUTHORS Wecker MS.
TITLE Direct Submission
JOURNAL Submitted (26-MAY-2016) Molecular Biology, GeneBiologics, 1047
Cherryvale Road, Boulder, CO 80303, USA
REFERENCE 3 (bases 1 to 7120)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 7120)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol.
Bioeng. (2016) In press"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(26-MAY-2016) Molecular Biology, GeneBiologics, 1047 Cherryvale
Road, Boulder, CO 80303, USA"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..7120
/mol_type="other DNA"
/organism="synthetic DNA construct"
CDS complement(264..794)
/label=GmR
/note="gentamycin acetyltransferase"
promoter complement(983..1011)
/label=Pc promoter
/note="class 1 integron promoter"
protein_bind 1281..1302
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 1317..1347
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 1355..1371
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 1379..1395
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
promoter 1416..1434
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
regulatory 1460..2094
/note="fru promoter; derived from Rhodobacter capsulatus"
/regulatory_class="promoter"
CDS 3844..3867
/label=Strep-Tag II
/note="peptide that binds Strep-Tactin(R), an engineered
form
of streptavidin"
promoter complement(3915..3933)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(3943..3959)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
CDS complement(4669..5328)
/label=pBBR1 Rep
/note="replication protein for the broad-host-range plasmid
pBBR1 from Bordetella bronchiseptica"
rep_origin complement(5329..6098)
/direction=LEFT
/label=pBBR1 oriV
/note="replication origin of the broad-host-range plasmid
pBBR1 from Bordetella bronchiseptica; requires the pBBR1
Rep protein for replication"
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