LOCUS       40924_32420        7120 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pMSW105, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7120)
  AUTHORS   Wecker MS, Beaton S, Chado R, Ghirardi ML.
  TITLE     Development of a Rhodobacter capsulatus self-reporting model system 
            for optimizing light-dependent, [FeFe]-hydrogenase-driven H 
            production
  JOURNAL   Biotechnol. Bioeng. (2016) In press
  PUBMED    27531314
REFERENCE   2  (bases 1 to 7120)
  AUTHORS   Wecker MS.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAY-2016) Molecular Biology, GeneBiologics, 1047 
            Cherryvale Road, Boulder, CO 80303, USA
REFERENCE   3  (bases 1 to 7120)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7120)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol.
            Bioeng. (2016) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (26-MAY-2016) Molecular Biology, GeneBiologics, 1047 Cherryvale 
            Road, Boulder, CO 80303, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7120
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(264..794)
                     /label=GmR
                     /note="gentamycin acetyltransferase"
     promoter        complement(983..1011)
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    1281..1302
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1317..1347
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1355..1371
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1379..1395
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1416..1434
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      1460..2094
                     /note="fru promoter; derived from Rhodobacter capsulatus"
                     /regulatory_class="promoter"
     CDS             3844..3867
                     /label=Strep-Tag II
                     /note="peptide that binds Strep-Tactin(R), an engineered 
                     form
                     of streptavidin"
     promoter        complement(3915..3933)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3943..3959)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(4669..5328)
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
     rep_origin      complement(5329..6098)
                     /direction=LEFT
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
ORIGIN
        1 accttcggga gcgcctgaag cccgttctgg acgccctggg gccgttgaat cgggatatgc
       61 aggccaaggc cgccgcgatc atcaaggccg tgggcgaaaa gctgctgacg gaacagcggg
      121 aagtccagcg ccagaaacag gcccagcgcc agcaggaacg cgggcgcgca catttccccg
      181 aaaagtgcca cctggcggcg ttgtgacaat ttaccgaaca actccgcggc cgggaagccg
      241 atctcggctt gaacgaattg ttaggtggcg gtacttgggt cgatatcaaa gtgcatcact
      301 tcttcccgta tgcccaactt tgtatagaga gccactgcgg gatcgtcacc gtaatctgct
      361 tgcacgtaga tcacataagc accaagcgcg ttggcctcat gcttgaggag attgatgagc
      421 gcggtggcaa tgccctgcct ccggtgctcg ccggagactg cgagatcata gatatagatc
      481 tcactacgcg gctgctcaaa cctgggcaga acgtaagccg cgagagcgcc aacaaccgct
      541 tcttggtcga aggcagcaag cgcgatgaat gtcttactac ggagcaagtt cccgaggtaa
      601 tcggagtccg gctgatgttg ggagtaggtg gctacgtctc cgaactcacg accgaaaaga
      661 tcaagagcag cccgcatgga tttgacttgg tcagggccga gcctacatgt gcgaatgatg
      721 cccatacttg agccacctaa ctttgtttta gggcgactgc cctgctgcgt aacatcgttg
      781 ctgctgcgta acatcgttgc tgctccataa catcaaacat cgacccacgg cgtaacgcgc
      841 ttgctgcttg gatgcccgag gcatagactg tacaaaaaaa cagtcataac aagccatgaa
      901 aaccgccact gcgccgttac caccgctgcg ttcggtcaag gttctggacc agttgcgtga
      961 gcgcatacgc tacttgcatt acagtttacg aaccgaacag gcttatgtca actgggttcg
     1021 tgccttcatc cgtttccacg gtgtgcgtcc atgggcaaat attatacgca aggcgacaag
     1081 gtgctgatgc cgctggcgat tcaggttcat catgccgttt gtgatggctt ccatgtcggc
     1141 agaatgctta atgaattaca acagttttta tgcatgcgcc caatacgcaa accgcctctc
     1201 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     1261 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
     1321 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
     1381 ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa
     1441 aagctgggta ccgggtacca agcttctcga gccccgccac gccccgggcg cggaaaaagg
     1501 cggcgatgcg ggcgtaatcc tgccatgtcc ggggcggttc cagcgcccgg ccggtctgcg
     1561 cccgataggc ggcctgcgcg gcccggtcct cgaacagatc gcggcgatag gcgcccatgt
     1621 gcagatcgcc gtccagcgtc accgccagcc actggccccg ccagcgcatc tgccggtcgc
     1681 gataggcggg gtggatgccc tgcaccagcg ggctttgcac aaggtcgtcc ggcaccggca
     1741 tcagataggg cgcgacatcg ggcagccagt cggcgggcag gatcagcaca tcggcggggg
     1801 ggctgccggt gacgaagccc atcatcaccg ccccgtaaag cgcgtcaaag gggcggggcg
     1861 tcacatgcac cgccacgccg gtttcgcgcg cgaactctgc cccatgggcg atggccgggc
     1921 ccgcaatcgg gccgccgtcg cgggtcagca cctcgagcgc ctcggcgcgg gtcaggctca
     1981 gcgccaatat gacaattgtt acaagccggg ccgtcacggg cgacatactc cattcatctg
     2041 ccgggcgctt cgtcgcctgc aagagcatcg cacatccgat gccgaggagg acatatgaaa
     2101 acgattatcc tgaacgggaa cgaagtgcac accgacaagg atatcaccat ccttgagctt
     2161 gcgcgggaaa acaatgtgga catcccgacc ctgtgcttcc tgaaggattg cgggaatttc
     2221 ggcaagtgcg gcgtgtgcat ggtggaggtg gaggggaagg gctttcgggc cgcctgcgtc
     2281 gcgaaagtgg aagatggcat ggtgatcaac accgagtcgg atgaagtgaa agaacgcatc
     2341 aagaagcgcg tgtccatgct cctggacaag catgagttca agtgcgggca atgctcccgg
     2401 cgcgagaact gcgagtttct gaagctggtg atcaagacga aggcgaaggc gagcaagccg
     2461 tttctgcccg aggataaaga cgcgctggtg gacaatcgct cgaaggcgat cgtcatcgat
     2521 cggtcgaaat gcgtgctgtg tggccggtgc gtcgccgcct gcaagcaaca tacctcgacg
     2581 tgctccatcc agttcatcaa gaaggacggg caacgcgccg tgggcaccgt ggacgatgtg
     2641 tgcctggatg attcgacctg cctgctgtgc ggccagtgcg tgatcgcgtg cccggtggcc
     2701 gcgctcaaag aaaagagcca tatcgaaaag gtccaggaag cgctcaacga cccgaagaag
     2761 catgtcatcg tcgcgatggc gcccagcgtc cggaccgcga tgggcgaact gtttaagatg
     2821 ggctatggca aagacgtcac cggcaagctg tacaccgccc tgcggatgct tggcttcgac
     2881 aaggtgttcg atatcaactt tggggccgat atgaccatca tggaagaagc caccgaactg
     2941 cttgggcgcg tcaagaacaa cgggcccttt ccgatgttca cgtcgtgctg tcccgcgtgg
     3001 gtccgcctgg cccagaacta tcacccggag cttctcgaca acctgagcag cgccaagtcg
     3061 ccgcagcaga tcttcggcac ggcctcgaaa acctattacc cctccatcag cggcattgcg
     3121 cccgaggatg tctatacggt cacgatcatg ccgtgcaacg acaagaagta tgaggcggat
     3181 atcccgttca tggaaaccaa ttcgctgcgg gatatcgacg cgtcgctgac cacgcgcgag
     3241 ctggccaaga tgatcaagga tgccaagatc aaattcgccg acctggagga cggggaagtg
     3301 gacccggcga tgggcacgta ctcgggcgcg ggggcgatct tcggcgcgac gggcggggtc
     3361 atggaggcgg ccatccgctc cgccaaagat tttgcggaga ataaagaact ggagaacgtg
     3421 gactacacgg aggtccgcgg cttcaagggc atcaaagagg ccgaggtgga gatcgcgggg
     3481 aacaagctca atgtggcggt gatcaatggg gccagcaact tcttcgagtt catgaagtcc
     3541 gggaagatga atgagaagca gtaccatttc attgaagtga tggcgtgtcc gggcggctgc
     3601 atcaatggcg gcggccagcc gcacgtgaac gcccttgacc gcgaaaacgt cgactatcgc
     3661 aagctccggg cgtcggtcct gtataaccag gacaaaaacg tcctgagcaa gcgcaagtcg
     3721 catgataacc cggcgatcat taagatgtac gacagctatt tcggcaaacc cggcgagggc
     3781 ctggcccaca agctgcttca cgtcaagtac accaaggaca agaacgtgtc gaagcatgag
     3841 aactggtcgc atccccagtt cgagaagtga actagttcta gagcggccgc caccgcggtg
     3901 gagctccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca
     3961 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
     4021 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
     4081 cagcctgaat ggcgaatgga aattgtaagc gttaatattt tgttaaaatt cgcgttaaat
     4141 ttttgttaaa tcagctcatt ttttaaccaa taggccgact gcgatgagtg gcagggcggg
     4201 gcgtaatttt tttaaggcag ttattggtgc ccttaaacgc ctggtgctac gcctgaataa
     4261 gtgataataa gcggatgaat ggcagaaatt cgaaagcaaa ttcgacccgg tcgtcggttc
     4321 agggcagggt cgttaaatag ccgcttatgt ctattgctgg tttaccggtt tattgactac
     4381 cggaagcagt gtgaccgtgt gcttctcaaa tgcctgaggc cagtttgctc aggctctccc
     4441 cgtggaggta ataattgacg atatgatcat ttattctgcc tcccagagcc tgataaaaac
     4501 ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat
     4561 gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgaggcg gctacagccg
     4621 atagtctgga acagcgcact tacgggttgc tgcgcaaccc aagtgctacc ggcgcggcag
     4681 cgtgacccgt gtcggcggct ccaacggctc gccatcgtcc agaaaacacg gctcatcggg
     4741 catcggcagg cgctgctgcc cgcgccgttc ccattcctcc gtttcggtca aggctggcag
     4801 gtctggttcc atgcccggaa tgccgggctg gctgggcggc tcctcgccgg ggccggtcgg
     4861 tagttgctgc tcgcccggat acagggtcgg gatgcggcgc aggtcgccat gccccaacag
     4921 cgattcgtcc tggtcgtcgt gatcaaccac cacggcggca ctgaacaccg acaggcgcaa
     4981 ctggtcgcgg ggctggcccc acgccacgcg gtcattgacc acgtaggccg acacggtgcc
     5041 ggggccgttg agcttcacga cggagatcca gcgctcggcc accaagtcct tgactgcgta
     5101 ttggaccgtc cgcaaagaac gtccgatgag cttggaaagt gtcttctggc tgaccaccac
     5161 ggcgttctgg tggcccatct gcgccacgag gtgatgcagc agcattgccg ccgtgggttt
     5221 cctcgcaata agcccggccc acgcctcatg cgctttgcgt tccgtttgca cccagtgacc
     5281 gggcttgttc ttggcttgaa tgccgatttc tctggactgc gtggccatgc ttatctccat
     5341 gcggtagggt gccgcacggt tgcggcacca tgcgcaatca gctgcaactt ttcggcagcg
     5401 cgacaacaat tatgcgttgc gtaaaagtgg cagtcaatta cagattttct ttaacctacg
     5461 caatgagcta ttgcgggggg tgccgcaatg agctgttgcg tacccccctt ttttaagttg
     5521 ttgattttta agtctttcgc atttcgccct atatctagtt ctttggtgcc caaagaaggg
     5581 cacccctgcg gggttccccc acgccttcgg cgcggctccc cctccggcaa aaagtggccc
     5641 ctccggggct tgttgatcga ctgcgcggcc ttcggccttg cccaaggtgg cgctgccccc
     5701 ttggaacccc cgcactcgcc gccgtgaggc tcggggggca ggcgggcggg cttcgccttc
     5761 gactgccccc actcgcatag gcttgggtcg ttccaggcgc gtcaaggcca agccgctgcg
     5821 cggtcgctgc gcgagccttg acccgccttc cacttggtgt ccaaccggca agcgaagcgc
     5881 gcaggccgca ggccggaggc ttttccccag agaaaattaa aaaaattgat ggggcaaggc
     5941 cgcaggccgc gcagttggag ccggtgggta tgtggtcgaa ggctgggtag ccggtgggca
     6001 atccctgtgg tcaagctcgt gggcaggcgc agcctgtcca tcagcttgtc cagcagggtt
     6061 gtccacgggc cgagcgaagc gagccagccg gtggccgctc gcggccatcg tccacatatc
     6121 cacgggctgg caagggagcg cagcgaccgc gcagggcgaa gcccggagag caagcccgta
     6181 gggcgccgca gccgccgtag gcggtcacga ctttgcgaag caaagtctag tgagtatact
     6241 caagcattga gtggcccgcc ggaggcaccg ccttgcgctg cccccgtcga gccggttgga
     6301 caccaaaagg gaggggcagg catggcggca tacgcgatca tgcgatgcaa gaagctggcg
     6361 aaaatgggca acgtggcggc cagtctcaag cacgcctacc gcgagcgcga gacgcccaac
     6421 gctgacgcca gcaggacgcc agagaacgag cactgggcgg ccagcagcac cgatgaagcg
     6481 atgggccgac tgcgcgagtt gctgccagag aagcggcgca aggacgctgt gttggcggtc
     6541 gagtacgtca tgacggccag cccggaatgg tggaagtcgg ccagccaaga acagcaggcg
     6601 gcgttcttcg agaaggcgca caagtggctg gcggacaagt acggggcgga tcgcatcgtg
     6661 acggccagca tccaccgtga cgaaaccagc ccgcacatga ccgcgttcgt ggtgccgctg
     6721 acgcaggacg gcaggctgtc ggccaaggag ttcatcggca acaaagcgca gatgacccgc
     6781 gaccagacca cgtttgcggc cgctgtggcc gatctagggc tgcaacgggg catcgagggc
     6841 agcaaggcac gtcacacgcg cattcaggcg ttctacgagg ccctggagcg gccaccagtg
     6901 ggccacgtca ccatcagccc gcaagcggtc gagccacgcg cctatgcacc gcagggattg
     6961 gccgaaaagc tgggaatctc aaagcgcgtt gagacgccgg aagccgtggc cgaccggctg
     7021 acaaaagcgg ttcggcaggg gtatgagcct gccctacagg ccgccgcagg agcgcgtgag
     7081 atgcgcaaga aggccgatca agcccaagag acggcccgag
//
