Basic Vector Information
- Vector Name:
- pMRI-37
- Antibiotic Resistance:
- Kanamycin
- Length:
- 4871 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Host:
- Yeast
- Source/Author:
- Xie W, Yu H.
- Promoter:
- TEF1
pMRI-37 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pMRI-37 vector Sequence
LOCUS 40924_32290 4871 bp DNA circular SYN 18-DEC-2018 DEFINITION Cloning vector pMRI-37, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4871) AUTHORS Xie W, Yu H. TITLE Developing a sequential repressing-inducing control strategy for improving carotenoids production in Saccharomyces cerevisiae JOURNAL Unpublished REFERENCE 2 (bases 1 to 4871) AUTHORS Xie W, Yu H. TITLE Direct Submission JOURNAL Submitted (26-FEB-2014) Department of Chemical and Biological Engineering, Zhejiang University, Institute of Bioengineering, 34 TianMuShan Road, Hangzhou, Zhejiang 310028, China REFERENCE 3 (bases 1 to 4871) TITLE Direct Submission REFERENCE 4 (bases 1 to 4871) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Unpublished" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (26-FEB-2014) Department of Chemical and Biological Engineering, Zhejiang University, Institute of Bioengineering, 34 TianMuShan Road, Hangzhou, Zhejiang 310028, China" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..4871 /mol_type="other DNA" /organism="synthetic DNA construct" protein_bind 22..55 /label=loxP /note="Cre-mediated recombination occurs in the 8-bp core sequence (ATGTATGC) (Shaw et al., 2021)." CDS complement(202..771) /gene="PTH1" /label=PTH1 /note="Peptidyl-tRNA hydrolase from Saccharomyces cerevisiae (strain ATCC 204508 / S288c). Accession#: P38876" misc_feature complement(796..1499) /label=ERG9 partial CDS region /note="ERG9 partial CDS region" regulatory complement(1497..1829) /label=BTS1 promoter derived from S. cerevisiae /note="BTS1 promoter derived from S. cerevisiae" /regulatory_class="promoter" promoter 1897..2304 /label=TEF1 promoter /note="promoter for EF-1-alpha" promoter 2325..2343 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" terminator 2384..2573 /label=CYC1 terminator /note="transcription terminator for CYC1" promoter complement(2586..2604) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" misc_feature 2627..2660 /label=Loxp site /note="Loxp site" protein_bind complement(2627..2660) /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." gene 2706..4062 /label=kanMX /note="yeast selectable marker conferring kanamycin resistance (Wach et al., 1994)" rep_origin complement(4195..4783) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
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