LOCUS       V004465                 4871 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004465
VERSION     V004465
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4871)
  AUTHORS   Xie W, Yu H.
  TITLE     Developing a sequential repressing-inducing control strategy for
            improving carotenoids production in Saccharomyces cerevisiae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4871)
  AUTHORS   Xie W, Yu H.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-FEB-2014) Department of Chemical and Biological
            Engineering, Zhejiang University, Institute of Bioengineering, 34
            TianMuShan Road, Hangzhou, Zhejiang 310028, China
REFERENCE   3  (bases 1 to 4871)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4871)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (26-FEB-2014) Department of Chemical and Biological Engineering,
            Zhejiang University, Institute of Bioengineering, 34 TianMuShan
            Road, Hangzhou, Zhejiang 310028, China"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4871
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    22..55
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(202..771)
                     /gene="PTH1"
                     /label="Peptidyl-tRNA hydrolase"
                     /note="Peptidyl-tRNA hydrolase from Saccharomyces
                     cerevisiae (strain ATCC 204508 / S288c). Accession#:
                     P38876"
     misc_feature    complement(796..1499)
                     /label="ERG9 partial CDS region"
                     /note="ERG9 partial CDS region"
     regulatory      complement(1497..1829)
                     /label="BTS1 promoter derived from S. cerevisiae"
                     /note="BTS1 promoter derived from S. cerevisiae"
                     /regulatory_class="promoter"
     promoter        1897..2304
                     /label="TEF1 promoter"
                     /note="promoter for EF-1-alpha"
     promoter        2325..2343
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      2384..2573
                     /label="CYC1 terminator"
                     /note="transcription terminator for CYC1"
     promoter        complement(2586..2604)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    2627..2660
                     /label="Loxp site"
                     /note="Loxp site"
     protein_bind    complement(2627..2660)
                     /label="loxP"
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (GCATACAT)."
     gene            2706..4062
                     /label="kanMX"
                     /note="yeast selectable marker conferring kanamycin
                     resistance (Wach et al., 1994)"
     rep_origin      complement(4195..4783)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 ggatcttcga gaacccttaa tataacttcg tataatgtat gctatacgaa gttattaggt
       61 gatatcagat ccacggatca ctaaagggaa caaaagctgg agctggcctt gtaggccgcc
      121 ttccgatgca aagtgcagtg gaaaagaaaa agggcaaagc aaataggatg gtaagtcggt
      181 attgttgttg aagatgggct atgaaatgta ctgagtcaga gcacgccagg cagcaggttc
      241 actctgtgta agcaaggttt gtagttcctg cggagttaga gctcccagaa cccaccggga
      301 cacgctcgca gggtctctag aacgggaccc aggttctctg ccgattccaa tagccaattt
      361 ggcaaagggt acacggcctc cactgcattt tagcaggctt cgcagcccat tatgacctct
      421 aatactggtg ctgggggctc tgagctgcac ttttccacac gccacactta actcgtcatg
      481 aataactacg tgtcgggctt ggtacttggc gtaccgggcc cagaccttgc acacctgttc
      541 tccacttaga ttcatgtact ggccatccga tcttagtatc gtacaatgtt cgtcttctat
      601 ataatgcact ttgccgcccg tattaggcac aggggaataa gttctcccct gcagaccaag
      661 ccgctttcgt agcagctcca gcatatatag ccctacattg tgacgggtac cagcgtactg
      721 aggctctgga ttgcctatcc cggtcagcac tagtctccat ttaccggaca tcttccttat
      781 ctgcctagag tcgaccaaat cttcattgta atctctgatg atgttggttt tttgtaggaa
      841 aagacccatg ctttcataca attgctcatt agaatacaaa gattcgttgg caaacttggc
      901 aatgacaatc aaacgggtca aaccatcacc gaccaaacca gctacgtagt gacagtacac
      961 gtcgtagtcg tggacggttt gcaacccatt caagttgtaa ttttcatcta agatgtagtc
     1021 ggccatacca ttacccattt tctcggtgat ctccttgatg acttcttgat attctggttt
     1081 caatttgtgg aattcaataa gaatcgattc gaaatctgtc aaaacggctc tgtccttcac
     1141 atcgggggca tttccgtcga aactccattt agttaacaac aatttctcgt ggaagtgacg
     1201 caacaagtca attttcaaat cgtgttcgat ggacatatcg tcttcgatgg tatccaaagc
     1261 ccttaaaatc aaataaaaga gagtaacaca gtttctcaat tctggatgca gctctctgat
     1321 cacagcagca aacgatctgg aggtcaagtt caacagttcg aaacagtgca agagatatgg
     1381 agacgtggac tgatcataga tggagaatag cggtgttctg caaaacttca gcttcaaagc
     1441 tgccttcatc tcgaccggat gcaatgccaa ttgtaatagc tttcccatgc ggccgctgat
     1501 tttccagact cgtaaacgct ttgttctctt tctattagta gcttctttga aaatgagcct
     1561 tacatggttt tgtaagcaca tctgtcttga cttttttttt gcttcaaaaa aacgtcaaag
     1621 aaggcaaggc gaagcaaaga aaaatggtag taagcataca atgatcagca taacatatgc
     1681 aaaaacacgt aatttctgct atgctggaag cggtgaacgt tggataccac tataataata
     1741 gaagtttacc tttcagtatg aaaatgcggg cttgtcctat atctatatgt aatattatgg
     1801 agtgagtaga gatggcggct atacatcgtc ctgcataccc ctcatttcca cgaccgcgaa
     1861 tccttacatc acacccaatc ccccacaagt gatcccccac acaccatagc ttcaaaatgt
     1921 ttctactcct tttttactct tccagatttt ctcggactcc gcgcatcgcc gtaccacttc
     1981 aaaacaccca agcacagcat actaaatttc ccctctttct tcctctaggg tgtcgttaat
     2041 tacccgtact aaaggtttgg aaaagaaaaa agagaccgcc tcgtttcttt ttcttcgtcg
     2101 aaaaaggcaa taaaaatttt tatcacgttt ctttttcttg aaaatttttt tttttgattt
     2161 ttttctcttt cgatgacctc ccattgatat ttaagttaat aaacggtctt caatttctca
     2221 agtttcagtt tcatttttct tgttctatta caactttttt tacttcttgc tcattagaaa
     2281 gaaagcatag caatctaatc taagttttaa ttacaaagga tccgtaatac gactcactat
     2341 agggcccggg cgtcgagtaa gcttggtacc gcggctagct aagatccgct ctaaccgaaa
     2401 aggaaggagt tagacaacct gaagtctagg tccctattta tttttttata gttatgttag
     2461 tattaagaac gttatttata tttcaaattt ttcttttttt tctgtacaga cgcgtgtacg
     2521 catgtaacat tatactgaaa accttgcttg agaaggtttt gggacgctcg aagatcccaa
     2581 ttcgccctat agtgagtcgt attacgcgcg ctcgacaacc cttaatataa cttcgtataa
     2641 tgtatgctat acgaagttat taggtctagt agcttgcctc gtccccgccg ggtcacccgg
     2701 ccagcgacat ggaggcccag aataccctcc ttgacagtct tgacgtgcgc agctcagggg
     2761 catgatgtga ctgtcgcccg tacatttagc ccatacatcc ccatgtataa tcatttgcat
     2821 ccatacattt tgatggccgc acggcgcgaa gcaaaaatta cggctcctcg ctgcagacct
     2881 gcgagcaggg aaacgctccc ctcacagacg cgttgaattg tccccacgcc gcgcccctgt
     2941 agagaaatat aaaaggttag gatttgccac tgaggttctt ctttcatata cttcctttta
     3001 aaatcttgct aggatacagt tctcacatca catccgaaca taaacaacca tgggtaagga
     3061 aaagactcac gtttcgaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta
     3121 taaatgggct cgcgataatg tcgggcaatc aggtgcgaca atctatcgat tgtatgggaa
     3181 gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac
     3241 agatgagatg gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca
     3301 ttttatccgt actcctgatg atgcatggtt actcaccact gcgatccccg gcaaaacagc
     3361 attccaggta ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt
     3421 gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt
     3481 atttcgtctc gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt
     3541 tgatgacgag cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataagctttt
     3601 gccattctca ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt
     3661 tgacgagggg aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata
     3721 ccaggatctt gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg
     3781 gctttttcaa aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat
     3841 gctcgatgag tttttctaat cagtactgac aataaaaaga ttcttgtttt caagaacttg
     3901 tcatttgtat agttttttta tattgtagtt gttctatttt aatcaaatgt tagcgtgatt
     3961 tatatttttt ttcgcctcga catcatctgc ccagatgcga agttaagtgc gcagaaagta
     4021 atatcatgcg tcaatcgtat gtgaatgctg gtcgctatac tgctgtcgat tcgatactaa
     4081 cgccgccatc cagtgtcgaa aacgaacaga atcaggggat aacgcaggaa agaacatgtg
     4141 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     4201 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     4261 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     4321 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     4381 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     4441 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     4501 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     4561 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     4621 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     4681 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     4741 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     4801 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     4861 attatcaaaa a
//
