Basic Vector Information
- Vector Name:
- pMQ97
- Antibiotic Resistance:
- Gentamicin
- Length:
- 9606 bp
- Type:
- Shuttle vector
- Replication origin:
- oriV
- Source/Author:
- Shanks RM, Caiazza NC, Hinsa SM, Toutain CM, O'Toole GA.
- Promoter:
- araBAD
pMQ97 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pMQ97 vector Sequence
LOCUS 40924_32190 9606 bp DNA circular SYN 18-DEC-2018 DEFINITION Shuttle vector pMQ97, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9606) AUTHORS Shanks RM, Caiazza NC, Hinsa SM, Toutain CM, O'Toole GA. TITLE Saccharomyces cerevisiae-based molecular tool kit for manipulation of genes from gram-negative bacteria JOURNAL Appl. Environ. Microbiol. 72 (7), 5027-5036 (2006) PUBMED 16820502 REFERENCE 2 (bases 1 to 9606) AUTHORS Shanks RM, Caiazza NC, Hinsa SM, Toutain CM, O'Toole GA. TITLE Direct Submission JOURNAL Submitted (18-MAY-2006) Microbiology and Immunology, Dartmouth Medical School, Vail Building Room 505, Hanover, NH 03755, USA REFERENCE 3 (bases 1 to 9606) TITLE Direct Submission REFERENCE 4 (bases 1 to 9606) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Appl. Environ. Microbiol."; date: "2006"; volume: "72"; issue: "7"; pages: "5027-5036" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (18-MAY-2006) Microbiology and Immunology, Dartmouth Medical School, Vail Building Room 505, Hanover, NH 03755, USA" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..9606 /mol_type="other DNA" /organism="synthetic DNA construct" CDS 1..801 /label=URA3 /note="orotidine-5'-phosphate decarboxylase, required for uracil biosynthesis" oriT complement(893..1001) /direction=LEFT /label=oriT /note="incP origin of transfer" rep_origin 1198..1728 /label=oriV /note="incP origin of replication" CDS 2120..3265 /label=trfA /note="trans-acting replication protein that binds to and activates oriV" terminator complement(3525..3611) /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" CDS complement(3841..4554) /label=yeGFP /note="yeast-enhanced green fluorescent protein" regulatory 4562..4567 /label=artificial RBS /note="artificial RBS" /regulatory_class="ribosome_binding_site" promoter complement(4612..4896) /label=araBAD promoter /note="promoter of the L-arabinose operon of E. coli; the araC regulatory gene is transcribed in the opposite direction (Guzman et al., 1995)" CDS 4923..5798 /label=araC /note="L-arabinose regulatory protein" rep_origin complement(6156..6744) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(6956..7486) /label=GmR /note="gentamycin acetyltransferase" promoter complement(7675..7703) /label=Pc promoter /note="class 1 integron promoter" rep_origin complement(7780..9122) /direction=LEFT /label=2u ori /note="yeast 2u plasmid origin of replication" promoter 9386..9606 /label=URA3 promoter
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