LOCUS       40924_32190        9606 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Shuttle vector pMQ97, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9606)
  AUTHORS   Shanks RM, Caiazza NC, Hinsa SM, Toutain CM, O'Toole GA.
  TITLE     Saccharomyces cerevisiae-based molecular tool kit for manipulation 
            of genes from gram-negative bacteria
  JOURNAL   Appl. Environ. Microbiol. 72 (7), 5027-5036 (2006)
  PUBMED    16820502
REFERENCE   2  (bases 1 to 9606)
  AUTHORS   Shanks RM, Caiazza NC, Hinsa SM, Toutain CM, O'Toole GA.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2006) Microbiology and Immunology, Dartmouth 
            Medical School, Vail Building Room 505, Hanover, NH 03755, USA
REFERENCE   3  (bases 1 to 9606)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9606)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Environ. Microbiol."; date: "2006"; volume: "72"; issue: "7"; pages:
            "5027-5036"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (18-MAY-2006) Microbiology and Immunology, Dartmouth Medical School,
            Vail Building Room 505, Hanover, NH 03755, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9606
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..801
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     oriT            complement(893..1001)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      1198..1728
                     /label=oriV
                     /note="incP origin of replication"
     CDS             2120..3265
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
     terminator      complement(3525..3611)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             complement(3841..4554)
                     /label=yeGFP
                     /note="yeast-enhanced green fluorescent protein"
     regulatory      4562..4567
                     /label=artificial RBS
                     /note="artificial RBS"
                     /regulatory_class="ribosome_binding_site"
     promoter        complement(4612..4896)
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     CDS             4923..5798
                     /label=araC
                     /note="L-arabinose regulatory protein"
     rep_origin      complement(6156..6744)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6956..7486)
                     /label=GmR
                     /note="gentamycin acetyltransferase"
     promoter        complement(7675..7703)
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     rep_origin      complement(7780..9122)
                     /direction=LEFT
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     promoter        9386..9606
                     /label=URA3 promoter
ORIGIN
        1 atgtcgaaag ctacatataa ggaacgtgct gctactcatc ctagtcctgt tgctgccaag
       61 ctatttaata tcatgcacga aaagcaaaca aacttgtgtg cttcattgga tgttcgtacc
      121 accaaggaat tactggagtt agttgaagca ttaggtccca aaatttgttt actaaaaaca
      181 catgtggata tcttgactga tttttccatg gagggcacag ttaagccgct aaaggcatta
      241 tccgccaagt acaatttttt actcttcgaa gacagaaaat ttgctgacat tggtaataca
      301 gtcaaattgc agtactctgc gggtgtatac agaatagcag aatgggcaga cattacgaat
      361 gcacacggtg tggtgggccc aggtattgtt agcggtttga agcaggcggc agaagaagta
      421 acaaaggaac ctagaggcct tttgatgtta gcagaattgt catgcaaggg ctccctatct
      481 actggagaat atactaaggg tactgttgac attgcgaaga gcgacaaaga ttttgttatc
      541 ggctttattg ctcaaagaga catgggtgga agagatgaag gttacgattg gttgattatg
      601 acacccggtg tgggtttaga tgacaaggga gacgcattgg gtcaacagta tagaaccgtg
      661 gatgatgtgg tctctacagg atctgacatt attattgttg gaagaggact atttgcaaag
      721 ggaagggatg ctaaggtaga gggtgaacgt tacagaaaag caggctggga agcatatttg
      781 agaagatgcg gccagcaaaa ctaaaaaact gtattataag taaatgcatg tatactaaac
      841 tcacaaatta gagcttcaat ttaattatat cagttattca cgtggagctt atcggccagc
      901 ctcgcagagc aggattcccg ttgagcaccg ccaggtgcga ataagggaca gtgaagaagg
      961 aacacccgct cgcgggtggg cctacttcac ctatcctgcc cggctgacgc cgttggatac
     1021 accaaggaaa gtctacacga accctttggc aaaatcctgt atatcgtgcg aaaaaggatg
     1081 gatataccga aaaaatcgct ataatgaccc cgaagcaggg ttatgcagcg gaaagtatac
     1141 cttaaccgcc ggctgacccg gcggggacga ggcaagctaa acagatctct agacctaggc
     1201 cgccggcgtt gtggatacct cgcggaaaac ttggccctca ctgacagatg aggggcggac
     1261 gttgacactt gaggggccga ctcacccggc gcggcgttga cagatgaggg gcaggctcga
     1321 tttcggccgg cgacgtggag ctggccagcc tcgcaaatcg gcgaaaacgc ctgattttac
     1381 gcgagtttcc cacagatgac gtggacaagc ctggggataa gtgccctgcg gtattgacac
     1441 ttgaggggcg cgactactga cagatgaggg gcgcgatcct tgacacttga ggggcagagt
     1501 gatgacagat gaggggcgca cctattgaca tttgaggggc tgtccacagg cagaaaatcc
     1561 agcatttgca agggtttccg cccgtttttc ggccaccgct aacctgtctt ttaacctgct
     1621 tttaaaccaa tatttataaa ccttgttttt aaccagggct gcgccctggc gcgtgaccgc
     1681 gcacgccgaa ggggggtgcc cccccttctc gaaccctccc ggcccgctaa cgcggcaccc
     1741 catcccccca ggggctgcgc ccctcggccg cgaacgacct caccccaaaa atggcagcca
     1801 cgtagaaagc cagtccgcag aaacggtgct gaccccggat gaatgtcagc tactgggcta
     1861 tctggacaag ggaaaacgca agcgcaaaga gaaagcaggt agcttgcagt gggcttacat
     1921 ggcgatagct agactgggcg gttttatgga cagcaagcga accggaattg ccagctgggg
     1981 cgccctctgg taaggttggg aagccctgca aagtaaactg gatggctttc ttgccgccaa
     2041 ggatctgatg gcgcagggga tcaagatcga cggatcgatc cggggaatta attccggggc
     2101 aatcccgcaa ggagggtgaa tgaatcggac gtttgaccgg aaggcataca ggcaagaact
     2161 gatcgacgcg gggttttccg ccgaggatgc cgaaaccatc gcaagccgca ccgtcatgcg
     2221 tgcgccccgc gaaaccttcc agtccgtcgg ctcgatggtc cagcaagcta cggccaagat
     2281 cgagcgcgac agcgtgcaac tggctccccc tgccctgccc gcgccatcgg ccgccgtgga
     2341 gcgttcgcgt cgtctcgaac aggaggcggc aggtttggcg aagtcgatga ccatcgacac
     2401 gcgaggaact atgacgacca agaagcgaaa aaccgccggc gaggacctgg caaaacaggt
     2461 cagcgaggcc aagcaggccg cgttgctgaa acacacgaag cagcagatca aggaaatgca
     2521 gctttccttg ttcgatattg cgccgtggcc ggacacgatg cgagcgatgc caaacgacac
     2581 ggcccgctct gccctgttca ccacgcgcaa caagaaaatc ccgcgcgagg cgctgcaaaa
     2641 caaggtcatt ttccacgtca acaaggacgt gaagatcacc tacaccggcg tcgagctgcg
     2701 ggccgacgat gacgaactgg tgtggcagca ggtgttggag tacgcgaagc gcacccctat
     2761 cggcgagccg atcaccttca cgttctacga gctttgccag gacctgggct ggtcgatcaa
     2821 tggccggtat tacacgaagg ccgaggaatg cctgtcgcgc ctacaggcga cggcgatggg
     2881 cttcacgtcc gaccgcgttg ggcacctgga atcggtgtcg ctgctgcacc gcttccgcgt
     2941 cctggaccgt ggcaagaaaa cgtcccgttg ccaggtcctg atcgacgagg aaatcgtcgt
     3001 gctgtttgct ggcgaccact acacgaaatt catatgggag aagtaccgca agctgtcgcc
     3061 gacggcccga cggatgttcg actatttcag ctcgcaccgg gagccgtacc cgctcaagct
     3121 ggaaaccttc cgcctcatgt gcggatcgga ttccacccgc gtgaagaagt ggcgcgagca
     3181 ggtcggcgaa gcctgcgaag agttgcgagg cagcggcctg gtggaacacg cctgggtcaa
     3241 tgatgacctg gtgcattgca aacgctaggg ccttgtgggg tcagttccgg ctgggggttc
     3301 agcagccacc tgcattaatt aactagaggc cgtggccacc acggcccggc ctgcctttca
     3361 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg
     3421 cgaaaggggg atgtgctgca aggatgcctg gcagtttatg gcgggcgtcc tgcccgccac
     3481 cctccgggcc gttgcttcgc aacgttcaaa tccgctcccg gcggatttgt cctactcagg
     3541 agagcgttca ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt
     3601 cgttttattt gatgcctggc agttccctac tctcgcatgg ggagacccca cactaccatc
     3661 ggcgctacgg cgtttcactt ctgagttcgg catggggtca ggtgggacca ccgcgctact
     3721 gccgccaggc aaattctgtt ttatcagacc gcttctgcgt tctgatttaa tctgtatcag
     3781 gctgaaaatc ttctctcatc cgccaaaaca gccaagcttg catgcctgca gactagtcta
     3841 tttgtatagt tcatccatgc catgtgtaat cccagcagct gttacaaact caagaaggac
     3901 catgtggtct ctcttttcgt tgggatcttt cgaaagggca gattgtgtgg acaggtaatg
     3961 gttgtctggt aaaaggacag ggccatcgcc aattggagta ttttgttgat aatggtctgc
     4021 tagttgaacg cttccatctt caatgttgtg tctaattttg aagttaactt tgattccatt
     4081 cttttgtttg tctgccatga tgtatacatt gtgtgagtta tagttgtatt ccaatttgtg
     4141 tccaagaatg tttccatctt ctttaaaatc aatacctttt aactcgattc tattaacaag
     4201 ggtatcacct tcaaacttga cttcagcacg tgtcttgtag ttcccgtcat ctttgaaaaa
     4261 tatagttctt tcctgtacat aaccttcggg catggcactc ttgaaaaagt catgctgttt
     4321 catatgatct gggtatctcg caaagcattg aacaccataa ccgaaagtag tgacaagtgt
     4381 tggccatgga acaggtagtt ttccagtagt gcaaataaat ttaagggtaa gttttccgta
     4441 tgttgcatca ccttcaccct ctccactgac agaaaatttg tgcccattaa catcaccatc
     4501 taattcaaca agaattggga caactccagt gaaaagttct tctcctttac tcatatgtat
     4561 atctccttcc ccgggtaccg agctcgaatt cgctagccca aaaaaacggg tatggagaaa
     4621 cagtagagag ttgcgataaa aagcgtcagg taggatccgc taatcttatg gataaaaatg
     4681 ctatggcata gcaaagtgtg acgccgtgca aataatcaat gtggactttt ctgccgtgat
     4741 tatagacact tttgttacgc gtttttgtca tggctttggt cccgctttgt tacagaatgc
     4801 ttttaataag cggggttacc ggtttggtta gcgagaagag ccagtaaaag acgcagtgac
     4861 ggcaatgtct gatgcaatat ggacaattgg tttcttctct gaatggcggg agtatgaaaa
     4921 gtatggctga agcgcaaaat gatcccctgc tgccgggata ctcgtttaat gcccatctgg
     4981 tggcgggttt aacgccgatt gaggccaacg gttatctcga tttttttatc gaccgaccgc
     5041 tgggaatgaa aggttatatt ctcaatctca ccattcgcgg tcagggggtg gtgaaaaatc
     5101 agggacgaga atttgtttgc cgaccgggtg atattttgct gttcccgcca ggagagattc
     5161 atcactacgg tcgtcatccg gaggctcgcg aatggtatca ccagtgggtt tactttcgtc
     5221 cgcgcgccta ctggcatgaa tggcttaact ggccgtcaat atttgccaat acggggttct
     5281 ttcgcccgga tgaagcgcac cagccgcatt tcagcgacct gtttgggcaa atcattaacg
     5341 ccgggcaagg ggaagggcgc tattcggagc tgctggcgat aaatctgctt gagcaattgt
     5401 tactgcggcg catggaagcg attaacgagt cgctccatcc accgatggat aatcgggtac
     5461 gcgaggcttg tcagtacatc agcgatcacc tggcagacag caattttgat atcgccagcg
     5521 tcgcacagca tgtttgcttg tcgccgtcgc gtctgtcaca tcttttccgc cagcagttag
     5581 ggattagcgt cttaagctgg cgcgaggacc aacgtatcag ccaggcgaag ctgcttttga
     5641 gcaccacccg gatgcctatc gccaccgtcg gtcgcaatgt tggttttgac gatcaactct
     5701 atttctcgcg ggtatttaaa aaatgcaccg gggccagccc gagcgagttc cgtgccggtt
     5761 gtgaagaaaa agtgaatgat gtagccgtca agttgtcata attggtaacg aatcagacaa
     5821 ttgacggctt gacggagtag catagggttt gcagaatccc tgcttcgtcc atttgacagg
     5881 cacattatgc atcgattaag ctgtcaaaca tgagcccagc tgcattaatg aatcggccaa
     5941 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     6001 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     6061 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     6121 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     6181 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     6241 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     6301 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     6361 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     6421 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     6481 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     6541 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
     6601 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     6661 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     6721 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     6781 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     6841 acctagatat gagtaaactt ggtctgacaa tcgatgcgaa ttggccgcgg cgttgtgaca
     6901 atttaccgaa caactccgcg gccgggaagc cgatctcggc ttgaacgaat tgttaggtgg
     6961 cggtacttgg gtcgatatca aagtgcatca cttcttcccg tatgcccaac tttgtataga
     7021 gagccactgc gggatcgtca ccgtaatctg cttgcacgta gatcacataa gcaccaagcg
     7081 cgttggcctc atgcttgagg agattgatga gcgcggtggc aatgccctgc ctccggtgct
     7141 cgccggagac tgcgagatca tagatataga tctcactacg cggctgctca aacctgggca
     7201 gaacgtaagc cgcgagagcg ccaacaaccg cttcttggtc gaaggcagca agcgcgatga
     7261 atgtcttact acggagcaag ttcccgaggt aatcggagtc cggctgatgt tgggagtagg
     7321 tggctacgtc tccgaactca cgaccgaaaa gatcaagagc agcccgcatg gatttgactt
     7381 ggtcagggcc gagcctacat gtgcgaatga tgcccatact tgagccacct aactttgttt
     7441 tagggcgact gccctgctgc gtaacatcgt tgctgctgcg taacatcgtt gctgctccat
     7501 aacatcaaac atcgacccac ggcgtaacgc gcttgctgct tggatgcccg aggcatagac
     7561 tgtacaaaaa aacagtcata acaagccatg aaaaccgcca ctgcgccgtt accaccgctg
     7621 cgttcggtca aggttctgga ccagttgcgt gagcgcatac gctacttgca ttacagttta
     7681 cgaaccgaac aggcttatgt caattcggtt gaatactcat actcttcaaa aataaacaaa
     7741 taggggttcc gcgcacattt ccccgaaaag tgccacctga acgaagcatc tgtgcttcat
     7801 tttgtagaac aaaaatgcaa cgcgagagcg ctaatttttc aaacaaagaa tctgagctgc
     7861 atttttacag aacagaaatg caacgcgaaa gcgctatttt accaacgaag aatctgtgct
     7921 tcatttttgt aaaacaaaaa tgcaacgcga gagcgctaat ttttcaaaca aagaatctga
     7981 gctgcatttt tacagaacag aaatgcaacg cgagagcgct attttaccaa caaagaatct
     8041 atacttcttt tttgttctac aaaaatgcat cccgagagcg ctatttttct aacaaagcat
     8101 cttagattac tttttttctc ctttgtgcgc tctataatgc agtctcttga taactttttg
     8161 cactgtaggt ccgttaaggt tagaagaagg ctactttggt gtctattttc tcttccataa
     8221 aaaaagcctg actccacttc ccgcgtttac tgattactag cgaagctgcg ggtgcatttt
     8281 ttcaagataa aggcatcccc gattatattc tataccgatg tggattgcgc atactttgtg
     8341 aacagaaagt gatagcgttg atgattcttc attggtcaga aaattatgaa cggtttcttc
     8401 tattttgtct ctatatacta cgtataggaa atgtttacat tttcgtattg ttttcgattc
     8461 actctatgaa tagttcttac tacaattttt ttgtctaaag agtaatacta gagataaaca
     8521 taaaaaatgt agaggtcgag tttagatgca agttcaagga gcgaaaggtg gatgggtagg
     8581 ttatataggg atatagcaca gagatatata gcaaagagat acttttgagc aatgtttgtg
     8641 gaagcggtat tcgcaatatt ttagtagctc gttacagtcc ggtgcgtttt tggttttttg
     8701 aaagtgcgtc ttcagagcgc ttttggtttt caaaagcgct ctgaagttcc tatactttct
     8761 agagaatagg aacttcggaa taggaacttc aaagcgtttc cgaaaacgag cgcttccgaa
     8821 aatgcaacgc gagctgcgca catacagctc actgttcacg tcgcacctat atctgcgtgt
     8881 tgcctgtata tatatataca tgagaagaac ggcatagtgc gtgtttatgc ttaaatgcgt
     8941 acttatatgc gtctatttat gtaggatgaa aggtagtcta gtacctcctg tgatattatc
     9001 ccattccatg cggggtatcg tatgcttcct tcagcactac cctttagctg ttctatatgc
     9061 tgccactcct caattggatt agtctcatcc ttcaatgcta tcatttcctt tgatattgga
     9121 tcatactaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     9181 ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
     9241 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
     9301 tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta
     9361 ctgagagtgc accataccac agcttttcaa ttcaattcat catttttttt ttattctttt
     9421 ttttgatttc ggtttctttg aaattttttt gattcggtaa tctccgaaca gaaggaagaa
     9481 cgaaggaagg agcacagact tagattggta tatatacgca tatgtagtgt tgaagaaaca
     9541 tgaaattgcc cagtattctt aacccaactg cacagaacaa aaacctgcag gaaacgaaga
     9601 taaatc
//
