pRPGSM vector (V003639)

LOCUS       40924_37608        4215 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pRPGSM, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4215)
  AUTHORS   Semsey S, Krishna S, Sneppen K, Adhya S.
  TITLE     Signal integration in the galactose network of Escherichia coli
  JOURNAL   Mol. Microbiol. 65 (2), 465-476 (2007)
  PUBMED    17630975
REFERENCE   2  (bases 1 to 4215)
  AUTHORS   Semsey S, Adhya S.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JAN-2007) Genetics, Eotvos Lorand University, Pazmany 
            P. S. 1/C, Budapest H-1117, Hungary
REFERENCE   3  (bases 1 to 4215)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4215)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol. 
            Microbiol."; date: "2007"; volume: "65"; issue: "2"; pages: 
            "465-476"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (22-JAN-2007) Genetics, Eotvos Lorand University, Pazmany P. S. 1/C,
            Budapest H-1117, Hungary"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4215
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..200
                     /label=AmpR promoter
     CDS             201..1058
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      1232..1820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     regulatory      complement(1917..1971)
                     /label=rpoC terminator
                     /note="rpoC terminator"
                     /regulatory_class="terminator"
     misc_feature    complement(1977..2257)
                     /label=mgl promoter and regulatory region
                     /note="mgl promoter and regulatory region"
     regulatory      complement(2290..2344)
                     /label=rpoC terminator
                     /note="rpoC terminator"
                     /regulatory_class="terminator"
     misc_feature    complement(2350..2649)
                     /label=galS promoter and regulatory region
                     /note="galS promoter and regulatory region"
     regulatory      complement(2682..2736)
                     /label=rpoC terminator
                     /note="rpoC terminator"
                     /regulatory_class="terminator"
     misc_feature    complement(2737..3042)
                     /label=galETKM promoter and regulatory region
                     /note="galETKM promoter and regulatory region"
     regulatory      complement(3074..3128)
                     /label=rpoC terminator
                     /note="rpoC terminator"
                     /regulatory_class="terminator"
     misc_feature    complement(3134..3463)
                     /label=galP promoter and regulatory region
                     /note="galP promoter and regulatory region"
     regulatory      complement(3486..3530)
                     /label=rpoC terminator
                     /note="rpoC terminator"
                     /regulatory_class="terminator"
     misc_feature    complement(3536..3767)
                     /label=galR promoter and regulatory region
                     /note="galR promoter and regulatory region"
     primer_bind     complement(3819..3835)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"