LOCUS       40924_37608        4215 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pRPGSM, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4215)
  AUTHORS   Semsey S, Krishna S, Sneppen K, Adhya S.
  TITLE     Signal integration in the galactose network of Escherichia coli
  JOURNAL   Mol. Microbiol. 65 (2), 465-476 (2007)
  PUBMED    17630975
REFERENCE   2  (bases 1 to 4215)
  AUTHORS   Semsey S, Adhya S.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JAN-2007) Genetics, Eotvos Lorand University, Pazmany 
            P. S. 1/C, Budapest H-1117, Hungary
REFERENCE   3  (bases 1 to 4215)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4215)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol. 
            Microbiol."; date: "2007"; volume: "65"; issue: "2"; pages: 
            "465-476"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (22-JAN-2007) Genetics, Eotvos Lorand University, Pazmany P. S. 1/C,
            Budapest H-1117, Hungary"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4215
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..200
                     /label=AmpR promoter
     CDS             201..1058
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      1232..1820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     regulatory      complement(1917..1971)
                     /label=rpoC terminator
                     /note="rpoC terminator"
                     /regulatory_class="terminator"
     misc_feature    complement(1977..2257)
                     /label=mgl promoter and regulatory region
                     /note="mgl promoter and regulatory region"
     regulatory      complement(2290..2344)
                     /label=rpoC terminator
                     /note="rpoC terminator"
                     /regulatory_class="terminator"
     misc_feature    complement(2350..2649)
                     /label=galS promoter and regulatory region
                     /note="galS promoter and regulatory region"
     regulatory      complement(2682..2736)
                     /label=rpoC terminator
                     /note="rpoC terminator"
                     /regulatory_class="terminator"
     misc_feature    complement(2737..3042)
                     /label=galETKM promoter and regulatory region
                     /note="galETKM promoter and regulatory region"
     regulatory      complement(3074..3128)
                     /label=rpoC terminator
                     /note="rpoC terminator"
                     /regulatory_class="terminator"
     misc_feature    complement(3134..3463)
                     /label=galP promoter and regulatory region
                     /note="galP promoter and regulatory region"
     regulatory      complement(3486..3530)
                     /label=rpoC terminator
                     /note="rpoC terminator"
                     /regulatory_class="terminator"
     misc_feature    complement(3536..3767)
                     /label=galR promoter and regulatory region
                     /note="galR promoter and regulatory region"
     primer_bind     complement(3819..3835)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg tgtgctgggc ccagccggcc agatctaaac tcgagatccc
     1921 taaactcccc ccataaaaaa acccgccgaa gcgggttttt acgttatttg ctgcagaagt
     1981 gttactgaac gccgaagctc actttttaaa attcgttctt ccatgcccgg taacgctcca
     2041 gaaaacggct ttaattgttg ttatgacgct gttacctcgg caaaagtgat tattcactgt
     2101 tacatacggg ttacaacgtt aaaacggtgc aatcatagct atcacattgt taagatactg
     2161 tgaaatcact cacagattga aagcggttac atcgcctgat ttgttgagtt agtgatcgcc
     2221 gccgcattct ggcgtgttgc gacagaatgg cgacgcagaa ttcatgcatg ctcggtaccc
     2281 gggtcgagat ccctaaactc cccccataaa aaaacccgcc gaagcgggtt tttacgttat
     2341 ttgctgcagt attgagcacc cgggaaaccg ttgccacaga gacgccagcc tgacgcgcta
     2401 catcacgaat ggtgatcata ttcactacct gttatgagat tgcagtaaat gactgcttgc
     2461 tggcggctat tttgtcagca ctgaatacag gacttcgtga atcgagtcac agcaatggaa
     2521 acggttacag ccgttttgtt aacgaatgtg atccagatcg ttatcttgat gttttagata
     2581 ttgccggtcc ggcagcacgc aaagttaact gacgccagag ccaatccacc ggcccctggc
     2641 ggaagtaacg aattcatgca tgctcggtac ccgggtcgag atccctaaac tccccccata
     2701 aaaaaacccg ccgaagcggg tttttacgtt atttgctgca gtaattgcac acaggtatga
     2761 cttccaatgt aaccgctacc accggtaacc agaactctca taattcgctc cattaggctt
     2821 atggtatgaa ataaccatag cataacaaag atgcgaaaag tgtgacatgg aataaattag
     2881 tggaatcgtt tacacaagaa tttagccgtt ttttatgcgc gattaagtga ttataaaaca
     2941 gagggtttat gaatgattgc gctttttatc tgaaaaaaga cgcggtttca tgcctgcatg
     3001 cgtcgaaccg ttggccggag agggtgctaa ggccgcctcc ggaattcatg catgctcggt
     3061 acccgggtcg agatccctaa actcccccca taaaaaaacc cgccgaagcg ggtttttacg
     3121 ttatttgctg caggcaagga agcagacgaa aaacgtcatt gccttgtttg accgcccctg
     3181 ttttttagcg tcaggcatga tgccctccaa tatggttatt ttttattgtg aattaagata
     3241 ggtgagtacg acgtaaaaag atgtgaagca aatcacatca gtgtaatcgc ttacactaac
     3301 atttatctta gttatgattt aagtgcatga aaataattga gttatatcat catagattaa
     3361 acgctgttat ctgcaattaa gactttactg aaaagaaatg taacaactgt gaaaaccgaa
     3421 taataatatg aaaatattac aaatctttgt ctgttgtggt tggaattccc ggggatccct
     3481 aaactccccc cataaaaaaa cccgccgaag cgggttttta cgttatttgc tgcagcggtg
     3541 gcgactgaaa cgcctgccag tcgggctaca tcctttatgg tcgccatgaa aataccttag
     3601 tgggtaaacg cttacattct ttcagtgtta cggaaaacgc ccgttggttc aaggggtggc
     3661 gtgttttcat cgtgggaatg tgagccaaac gcaaataagt aagacaaaaa aagagattgc
     3721 aaacctttgg ttacactttg cgaaacgctg ttgcgattga ccgctggaat tcatgcatgc
     3781 tcggtacccg ggtcgacctg caggcatgca agcttggcac tggccgtcgt tttacaacgt
     3841 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     3901 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     3961 ctgaatggcg aatggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
     4021 caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc
     4081 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
     4141 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
     4201 caccgaaacg cgcga
//
