pRN-FRT vector (V003663)

LOCUS       V003663                 4100 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003663
VERSION     V003663
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4100)
  AUTHORS   Liu X.
  TITLE     A Highly Efficient and Simple Construction Strategy for Producing
            Recombinant Baculovirus Bombyx mori Nucleopolyhedrovirus
  JOURNAL   PLoS ONE 11 (3), E0152140 (2016)
   PUBMED   27008267
REFERENCE   2  (bases 1 to 4100)
  AUTHORS   Liu X.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2016) Biotechnology Research Institute, Chinese
            Academy of Agricultural Sciences, No. 12 Zhongguancun South Street,
            Beijing, Beijing 100081, China
REFERENCE   3  (bases 1 to 4100)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4100)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE";
            date: "2016"; volume: "11"; issue: "3"; pages: "E0152140"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (21-FEB-2016) Biotechnology Research Institute, Chinese Academy of
            Agricultural Sciences, No. 12 Zhongguancun South Street, Beijing,
            Beijing 100081, China"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4100
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    427..474
                     /label="FRT site"
                     /note="FRT site"
     protein_bind    complement(427..474)
                     /label="FRT"
                     /bound_moiety="FLP recombinase from the Saccharomyces
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             613..984
                     /gene="rpsL"
                     /label="Small ribosomal subunit protein uS12"
                     /note="Small ribosomal subunit protein uS12 from Salmonella
                     paratyphi A (strain ATCC 9150 / SARB42). Accession#:
                     Q5PIW1"
     CDS             999..1790
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     protein_bind    complement(1794..1827)
                     /label="FRT (minimal)"
                     /note="supports FLP-mediated excision but not integration
                     (Turan and Bode, 2011)"
     primer_bind     complement(1879..1895)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(1903..1919)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1927..1957)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1972..1993)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2281..2869)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3043..3900)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3901..4005)
                     /label="AmpR promoter"