LOCUS       V003663                 4100 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003663
VERSION     V003663
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4100)
  AUTHORS   Liu X.
  TITLE     A Highly Efficient and Simple Construction Strategy for Producing
            Recombinant Baculovirus Bombyx mori Nucleopolyhedrovirus
  JOURNAL   PLoS ONE 11 (3), E0152140 (2016)
   PUBMED   27008267
REFERENCE   2  (bases 1 to 4100)
  AUTHORS   Liu X.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2016) Biotechnology Research Institute, Chinese
            Academy of Agricultural Sciences, No. 12 Zhongguancun South Street,
            Beijing, Beijing 100081, China
REFERENCE   3  (bases 1 to 4100)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4100)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE";
            date: "2016"; volume: "11"; issue: "3"; pages: "E0152140"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (21-FEB-2016) Biotechnology Research Institute, Chinese Academy of
            Agricultural Sciences, No. 12 Zhongguancun South Street, Beijing,
            Beijing 100081, China"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4100
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    427..474
                     /label="FRT site"
                     /note="FRT site"
     protein_bind    complement(427..474)
                     /label="FRT"
                     /bound_moiety="FLP recombinase from the Saccharomyces
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             613..984
                     /gene="rpsL"
                     /label="Small ribosomal subunit protein uS12"
                     /note="Small ribosomal subunit protein uS12 from Salmonella
                     paratyphi A (strain ATCC 9150 / SARB42). Accession#:
                     Q5PIW1"
     CDS             999..1790
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     protein_bind    complement(1794..1827)
                     /label="FRT (minimal)"
                     /note="supports FLP-mediated excision but not integration
                     (Turan and Bode, 2011)"
     primer_bind     complement(1879..1895)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(1903..1919)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1927..1957)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1972..1993)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2281..2869)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3043..3900)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3901..4005)
                     /label="AmpR promoter"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttgcatgc ctgcaggtcg
      421 acgattgaag ttcatatact ttctagagaa taggaacttc ggaataggaa cttcggcctg
      481 gtgatgatgg cgggatcgtt gtatatttct tgacaccttt tcggcatcgc cctaaaattc
      541 ggcgtcctca tattgtgtga ggacgtttta ttacgtgttt acgaagcaaa agctaaaacc
      601 aggagctatt taatggcaac agttaaccag ctggtacgca aaccacgtgc tcgcaaagtt
      661 gcgaaaagca acgtgcctgc gctggaagca tgcccgcaaa aacgtggcgt atgtactcgt
      721 gtatatacta ccactcctaa aaaaccgaac tccgcgctgc gtaaagtatg ccgtgttcgt
      781 ctgactaacg gtttcgaagt gacttcctac atcggtggtg aaggtcacaa cctgcaggag
      841 cactccgtga tcctgatccg tggcggtcgt gttaaagacc tcccgggtgt tcgttaccac
      901 accgtacgtg gtgcgcttga ctgctccggc gttaaagacc gtaagcaggc tcgttccaag
      961 tatggcgtga agcgtcctaa ggcttaagga ggacaatcat gattgaacaa gatggattgc
     1021 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
     1081 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
     1141 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat
     1201 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
     1261 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
     1321 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
     1381 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
     1441 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
     1501 ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc
     1561 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
     1621 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
     1681 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
     1741 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagaagttc
     1801 ctatactttc tagagaatat gaacttcgga ataatctcta gaggatcccc gggtaccgag
     1861 ctcgaattcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     1921 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     1981 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     2041 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     2101 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     2161 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     2221 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     2281 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     2341 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     2401 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     2461 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     2521 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     2581 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     2641 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     2701 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     2761 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     2821 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     2881 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     2941 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     3001 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     3061 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     3121 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     3181 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     3241 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     3301 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     3361 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     3421 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     3481 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     3541 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     3601 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     3661 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     3721 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     3781 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     3841 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     3901 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     3961 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     4021 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     4081 tatcacgagg ccctttcgtc
//
