pRML2 vector (V003673)

LOCUS       40924_37413        5180 bp DNA     circular SYN 18-DEC-2018
DEFINITION  YAC construction vector pRML2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5180)
  AUTHORS   Knight TF Jr.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-OCT-2006) CSAIL, MIT, 32 Vassar St, Cambridge, MA 
            02139, USA
REFERENCE   2  (bases 1 to 5180)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 5180)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (03-OCT-2006) CSAIL, MIT, 32 Vassar St, Cambridge, MA 02139, USA"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5180
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(68..86)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    104..485
                     /label=ARSH4 region
                     /note="ARSH4 region"
     CDS             complement(633..1433)
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     promoter        complement(1434..1649)
                     /label=URA3 promoter
     primer_bind     complement(1678..1694)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      1836..2291
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2317..2421
                     /label=AmpR promoter
     CDS             2422..3279
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      3453..4041
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    4329..4350
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4365..4395
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4403..4419
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4427..4443
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4469..4485
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    4529..5161
                     /note="telomere region from pYAC4 of GenBank Accession
                     Number U01086"