LOCUS       40924_37413        5180 bp DNA     circular SYN 18-DEC-2018
DEFINITION  YAC construction vector pRML2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5180)
  AUTHORS   Knight TF Jr.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-OCT-2006) CSAIL, MIT, 32 Vassar St, Cambridge, MA 
            02139, USA
REFERENCE   2  (bases 1 to 5180)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 5180)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (03-OCT-2006) CSAIL, MIT, 32 Vassar St, Cambridge, MA 02139, USA"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5180
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(68..86)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    104..485
                     /label=ARSH4 region
                     /note="ARSH4 region"
     CDS             complement(633..1433)
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     promoter        complement(1434..1649)
                     /label=URA3 promoter
     primer_bind     complement(1678..1694)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      1836..2291
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2317..2421
                     /label=AmpR promoter
     CDS             2422..3279
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      3453..4041
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    4329..4350
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4365..4395
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4403..4419
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4427..4443
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4469..4485
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    4529..5161
                     /note="telomere region from pYAC4 of GenBank Accession
                     Number U01086"
ORIGIN
        1 atcgatttcg aagctagctc gcgagtcgac cgcggccgca gatctgaatt cgatgtaccc
       61 aattcgccct atagtgagtc gtattacgcg cgtcgacggt atcgggggat cgccaacaaa
      121 tactaccttt tatcttgctc ttcctgctct caggtattaa tgccgaattg tttcatcttg
      181 tctgtgtaga agaccacaca cgaaaatcct gtgattttac attttactta tcgttaatcg
      241 aatgtatatc tatttaatct gcttttcttg tctaataaat atatatgtaa agtacgcttt
      301 ttgttgaaat tttttaaacc tttgtttatt tttttttctt cattccgtaa ctcttctacc
      361 ttctttattt actttctaaa atccaaatac aaaacataaa aataaataaa cacagagtaa
      421 attcccaaat tattccatca ttaaaagata cgaggcgcgt gtaagttaca ggcaagcgat
      481 ccgtcgataa gctttttctt tccaattttt tttttttcgt cattataaaa atcattacga
      541 ccgagattcc cgggtaataa ctgatataat taaattgaag ctctaatttg tgagtttagt
      601 atacatgcat ttacttataa tacagttttt tagttttgct ggccgcatct tctcaaatat
      661 gcttcccagc ctgcttttct gtaacgttca ccctctacct tagcatccct tccctttgca
      721 aatagtcctc ttccaacaat aataatgtca gatcctgtag agaccacatc atccacggtt
      781 ctatactgtt gacccaatgc gtctcccttg tcatctaaac ccacaccggg tgtcataatc
      841 aaccaatcgt aaccttcatc tcttccaccc atgtctcttt gagcaataaa gccgataaca
      901 aaatctttgt cgctcttcgc aatgtcaaca gtacccttag tatattctcc agtagatagg
      961 gagcccttgc atgacaattc tgctaacatc aaaaggcctc taggttcctt tgttacttct
     1021 tctgccgcct gcttcaaacc gctaacaata cctgggccca ccacaccgtg tgcattcgta
     1081 atgtctgccc attctgctat tctgtataca cccgcagagt actgcaattt gactgtatta
     1141 ccaatgtcag caaattttct gtcttcgaag agtaaaaaat tgtacttggc ggataatgcc
     1201 tttagcggct taactgtgcc ctccatggaa aaatcagtca agatatccac atgtgttttt
     1261 agtaaacaaa ttttgggacc taatgcttca actaactcca gtaattcctt ggtggtacga
     1321 acatccaatg aagcacacaa gtttgtttgc ttttcgtgca tgatattaaa tagcttggca
     1381 gcaacaggac taggatgagt agcagcacgt tccttatatg tagctttcga catgatttat
     1441 cttcgtttcc tgcaggtttt tgttctgtgc agttgggtta agaatactgg gcaatttcat
     1501 gtttcttcaa cactacatat gcgtatatat accaatctaa gtctgtgctc cttccttcgt
     1561 tcttccttct gttcggagat taccgaatca aaaaaatttc aaggaaaccg aaatcaaaaa
     1621 aaagaataaa aaaaaaatga tgaattgaaa agcttgatat cgaattaatt cgcgctcact
     1681 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
     1741 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
     1801 ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag
     1861 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     1921 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     1981 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     2041 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     2101 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     2161 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     2221 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
     2281 gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     2341 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     2401 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     2461 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     2521 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     2581 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     2641 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     2701 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     2761 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     2821 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     2881 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     2941 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     3001 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     3061 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     3121 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     3181 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     3241 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     3301 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     3361 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     3421 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     3481 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     3541 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc
     3601 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     3661 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     3721 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     3781 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     3841 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     3901 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     3961 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     4021 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     4081 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     4141 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     4201 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     4261 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     4321 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
     4381 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat
     4441 gaccatgatt acgccaagcg cgggccgctc tagaactagt ggatcctcta gagtcgatcg
     4501 acctgcaggc atgcctgcag gtcgaggcga taagcttcat ttttagataa aatttattaa
     4561 tcatcattaa tttcttgaaa aacattttat ttattgatct tttataacaa aaaacccttc
     4621 taaaagttta tttttgaatg aaaaacttat aaaaatttat gaaaactaca aaaaataaaa
     4681 tttttaatta aaataatttt gataagaact tcaatctttg actagcttag tcatttttga
     4741 gatttaatta atattttatg tttattcata tataaactat tcaaaatatt atagaattta
     4801 aacattttaa catcttaatc attcataaat aactaaaaat caaagtatta cattcaataa
     4861 ataactttta ctcaatgtca aagaaattat tggggttggg gttggggttg gggttggggt
     4921 tggggttggg gttggggttg gggttggggt tggggttggg gttggggttg gggttggggt
     4981 tggggttggg gttggggttg gggttggggt tggggttggg gttggggttg gggttggggt
     5041 tggggttggg gttggggttg gggttggggt tggggttggg gttggggttg ggggttgggg
     5101 ttggggttgg ggttggggtt ggggttgggg ttggggttgg ggttggggtt ggggttgggg
     5161 tctgcctcgc gcggaaattc
//
