pRL2665b vector (V003712)

LOCUS       V003712                 6400 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003712
VERSION     V003712
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6400)
  AUTHORS   Huang G, Fan Q, Lechno-Yossef S, Wojciuch E, Wolk CP, Kaneko T,
            Tabata S.
  TITLE     Clustered genes required for the synthesis of heterocyst envelope
            polysaccharide in Anabaena sp. strain PCC 7120
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6400)
  AUTHORS   Wolk CP.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JUN-2004) MSU-DOE Plant Research Laboratory, Michigan
            State University, Wilson Road, E. Lansing, MI 48824, USA
REFERENCE   3  (bases 1 to 6400)
  AUTHORS   Wolk CP.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2012) MSU-DOE Plant Research Laboratory, Michigan
            State University, Wilson Road, E. Lansing, MI 48824, USA
REFERENCE   4  (bases 1 to 6400)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 6400)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (19-JUN-2004) MSU-DOE Plant Research Laboratory, Michigan State
            University, Wilson Road, E. Lansing, MI 48824, USA"
            SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
            (01-MAR-2012) MSU-DOE Plant Research Laboratory, Michigan State
            University, Wilson Road, E. Lansing, MI 48824, USA"
            SGRef: number: 4; type: "Journal Article"
            On Mar 1, 2012 this sequence version replaced AY661563.1.
FEATURES             Location/Qualifiers
     source          1..6400
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        104..208
                     /label="AmpR promoter"
     misc_feature    560..700
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(886..1474)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(1761..2552)
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(3377..3481)
                     /label="AmpR promoter"
     promoter        3788..3890
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             3891..4547
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     CDS             4804..5535
                     /gene="ermC"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from
                     Staphylococcus aureus. Accession#: P02979"
     CDS             complement(5700..6068)
                     /label="traJ"
                     /note="oriT-recognizing protein"
     oriT            complement(6101..6210)
                     /direction=LEFT
                     /label="oriT"
                     /note="incP origin of transfer"