LOCUS       V003712                 6400 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003712
VERSION     V003712
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6400)
  AUTHORS   Huang G, Fan Q, Lechno-Yossef S, Wojciuch E, Wolk CP, Kaneko T,
            Tabata S.
  TITLE     Clustered genes required for the synthesis of heterocyst envelope
            polysaccharide in Anabaena sp. strain PCC 7120
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6400)
  AUTHORS   Wolk CP.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JUN-2004) MSU-DOE Plant Research Laboratory, Michigan
            State University, Wilson Road, E. Lansing, MI 48824, USA
REFERENCE   3  (bases 1 to 6400)
  AUTHORS   Wolk CP.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2012) MSU-DOE Plant Research Laboratory, Michigan
            State University, Wilson Road, E. Lansing, MI 48824, USA
REFERENCE   4  (bases 1 to 6400)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 6400)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (19-JUN-2004) MSU-DOE Plant Research Laboratory, Michigan State
            University, Wilson Road, E. Lansing, MI 48824, USA"
            SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
            (01-MAR-2012) MSU-DOE Plant Research Laboratory, Michigan State
            University, Wilson Road, E. Lansing, MI 48824, USA"
            SGRef: number: 4; type: "Journal Article"
            On Mar 1, 2012 this sequence version replaced AY661563.1.
FEATURES             Location/Qualifiers
     source          1..6400
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        104..208
                     /label="AmpR promoter"
     misc_feature    560..700
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(886..1474)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(1761..2552)
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(3377..3481)
                     /label="AmpR promoter"
     promoter        3788..3890
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             3891..4547
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     CDS             4804..5535
                     /gene="ermC"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from
                     Staphylococcus aureus. Accession#: P02979"
     CDS             complement(5700..6068)
                     /label="traJ"
                     /note="oriT-recognizing protein"
     oriT            complement(6101..6210)
                     /direction=LEFT
                     /label="oriT"
                     /note="incP origin of transfer"
ORIGIN
        1 ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
       61 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
      121 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
      181 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
      241 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
      301 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
      361 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttct
      421 gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
      481 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
      541 gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata
      601 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga
      661 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct
      721 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
      781 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
      841 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
      901 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
      961 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     1021 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     1081 atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     1141 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     1201 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     1261 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     1321 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     1381 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     1441 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     1501 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     1561 aaggatcttc acctagatcc ttttggggtg ggcgaagaac tccagcatga gatccccgcg
     1621 ctggaggatc atccagccgg cgtcccggaa aacgattccg aagcccaacc tttcatagaa
     1681 ggcggcggtg gaatcgaaat ctcgtgatgg caggttgggc gtcgcttggt cggtcatttc
     1741 gaaccccaga gtcccgctca gaagaactcg tcaagaaggc gatagaaggc gatgcgctgc
     1801 gaatcgggag cggcgatacc gtaaagcacg aggaagcggt cagcccattc gccgccaagc
     1861 tcttcagcaa tatcacgggt agccaacgct atgtcctgat agcggtccgc cacacccagc
     1921 cggccacagt cgatgaatcc agaaaagcgg ccattttcca ccatgatatt cggcaagcag
     1981 gcatcgccat gggtcacgac gagatcctcg ccgtcgggca tccgcgcctt gagcctggcg
     2041 aacagttcgg ctggcgcgag cccctgatgc tcttcgtcca gatcatcctg atcgacaaga
     2101 ccggcttcca tccgagtacg tgctcgctcg atgcgatgtt tcgcttggtg gtcgaatggg
     2161 caggtagccg gatcaagcgt atgcagccgc cgcattgcat cagccatgat ggatactttc
     2221 tcggcaggag caaggtgaga tgacaggaga tcctgccccg gcacttcgcc caatagcagc
     2281 cagtcccttc ccgcttcagt gacaacgtcg agcacagctg cgcaaggaac gcccgtcgtg
     2341 gccagccacg atagccgcgc tgcctcgtct tggagttcat tcagggcacc ggacaggtcg
     2401 gtcttgacaa aaagaaccgg gcgcccctgc gctgacagcc ggaacacggc ggcatcagag
     2461 cagccgattg tctgttgtgc ccagtcatag ccgaatagcc tctccaccca agcggccgga
     2521 gaacctgcgt gcaatccatc ttgttcaatc atgcgaaacg atcctcatcc tgtctcttga
     2581 tcagatcttg atcccctgcg ccatcagatc cttggcggca agaaagccat ccagtttact
     2641 ttgcagggct tcccaacctt accagagggc gccccagctg gcaattccgg ttcgcttgct
     2701 gtccataaaa ccgcccagtc tagctatcgc catgtaagcc cactgcaagc tacctgcttt
     2761 ctctttgcgc ttgcgttttc ccttgtccag atagcccagt agctgacatt catccggggt
     2821 cagcaccgtt tctgcggact ggctttctac gtgttccgct tcctttagca gcccttgcgc
     2881 cctgagtgct tgcggcagcg tgaagctagc ttatcgcgcc attcgccatt caggctgcgc
     2941 aactgttggg aagggcgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     3001 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     3061 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     3121 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     3181 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     3241 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     3301 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     3361 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     3421 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     3481 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
     3541 cctataaaaa taggcgtatc acgaggccct ttcgtcttca agaattcatc gatatctaga
     3601 tctcgagctc gcgaaagctt gcatgcctgc aggtccccgg gtaccgagct agagtctttg
     3661 ttttgacgcc attagcgtac atcgaataaa tacctgtgac ggaagatcac ttcgcagaat
     3721 aaataaatcc tggtgtccct gttgataccg ggaagccctg ggccaacttt tggcgaaaat
     3781 gagacgttga tcggcacgta agaggttcca actttcacca taatgaaata agatcactac
     3841 cgggcgtatt ttttgagtta tcgagatttt caggagctaa ggaagctaaa atggagaaaa
     3901 aaatcactgg atataccacc gttgatatat cccaatggca tcgtaaagaa cattttgagg
     3961 catttcagtc agttgctcaa tgtacctata accagaccgt tcagctggat attacggcct
     4021 ttttaaagac cgtaaagaaa aataagcaca agttttatcc ggcctttatt cacattcttg
     4081 cccgcctgat gaatgctcat ccggaattcc gtatggcaat gaaagacggt gagctggtga
     4141 tatgggatag tgttcaccct tgttacaccg ttttccatga gcaaactgaa acgttttcat
     4201 cgctctggag tgaataccac gacgatttcc ggcagtttct acacatatat tcgcaagatg
     4261 tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg gtttattgag aatatgtttt
     4321 tcgtctcagc caatccctgg gtgagtttca ccagttttga tttaaacgtg gccaatatgg
     4381 acaacttctt cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc
     4441 tgatgccgct ggcgattcag gttcatcatg ccgtttgtga tggcttccat gtcggcagaa
     4501 tgcttaatga attacaacag tactgcgatg agtggcaggg cggggcgtaa tttttttaag
     4561 gcagttattg gtgcccttaa acgcctggtg ctacgcctga ataagtgata ataagcggat
     4621 gaatggcaga aattcgatga attcgcgtgc tataattata ctaattttat aaggaggaaa
     4681 aaatatgggc atttttagta tttttgtaat cagcacagtt cattatcaac caaacaaaaa
     4741 ataagtggtt ataatgaatc gttaataagc aaaattcata taaccaaatt aaagagggtt
     4801 ataatgaacg agaaaaatat aaaacacagt caaaacttta ttacttcaaa acataatata
     4861 gataaaataa tgacaaatat aagattaaat gaacatgata atatctttga aatcggctca
     4921 ggaaaaggcc attttaccct tgaattagta aagaggtgta atttcgtaac tgccattgaa
     4981 atagaccata aattatgcaa aactacagaa aataaacttg ttgatcacga taatttccaa
     5041 gttttaaaca aggatatatt gcagtttaaa tttcctaaaa accaatccta taaaatatat
     5101 ggtaatatac cttataacat aagtacggat ataatacgca aaattgtttt tgatagtata
     5161 gctaatgaga tttatttaat cgtggaatac gggtttgcta aaagattatt aaatacaaaa
     5221 cgctcattgg cattactttt aatggcagaa gttgatattt ctatattaag tatggttcca
     5281 agagaatatt ttcatcctaa acctaaagtg aatagctcac ttatcagatt aagtagaaaa
     5341 aaatcaagaa tatcacacaa agataaacaa aagtataatt atttcgttat gaaatgggtt
     5401 aacaaagaat acaagaaaat atttacaaaa aatcaattta acaattcctt aaaacatgca
     5461 ggaattgacg atttaaacaa tattagcttt gaacaattct tatctctttt caatagctat
     5521 aaattattta ataagtaagt taagggatgc aggtcgactc ggtacccggg atcgtcttgc
     5581 cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg atggagcgca
     5641 tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta aaaaagtcat
     5701 ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc ctgcttctct
     5761 tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt gcgcgggtcg
     5821 tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt gatgcgggcc
     5881 agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg gccgacggcc
     5941 agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat cgctcttcgt
     6001 tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg cttggtttca
     6061 tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc tcgcagagca
     6121 ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga acacccgctc
     6181 gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca ccaaggaaag
     6241 tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg atataccgaa
     6301 aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aagatcgtcg acctgcaggc
     6361 atgcaagctt tcgcgagctc gagatctaga tatcgatgaa
//
