praAqp4-Luc vector (V003836)

Basic Vector Information

      • Vector Name:
      • praAqp4-Luc
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 6181 bp
      • Type:
      • Reporter vector
      • Replication origin:
      • ori
      • Source/Author:
      • Holen T, Moe SE, Sorbo JG, Meza TJ, Ottersen OP, Klungland A.

praAqp4-Luc vector Vector Map

praAqp4-Luc6181 bp30060090012001500180021002400270030003300360039004200450048005100540057006000SV40 promoterAqp4SV40 poly(A) signaloriAmpRAmpR promoterf1 oripoly(A) signalpause site

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

praAqp4-Luc vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_36303        6181 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Reporter vector praAqp4-Luc, complete sequence.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6181)
  AUTHORS   Holen T, Moe SE, Sorbo JG, Meza TJ, Ottersen OP, Klungland A.
  TITLE     Tolerated wobble mutations in siRNAs decrease specificity, but can 
            enhance activity in vivo
  JOURNAL   Nucleic Acids Res. 33 (15), 4704-4710 (2005)
  PUBMED    16113241
REFERENCE   2  (bases 1 to 6181)
  AUTHORS   Holen T, Ottersen OP, Klungland A.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2004) CMBN, University of Oslo, Sognsvannsveien 9,
            Oslo 0317, Norway
REFERENCE   3  (bases 1 to 6181)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6181)
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2005"; volume: "33"; issue: "15"; pages: 
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (15-OCT-2004) CMBN, University of Oslo, Sognsvannsveien 9, Oslo 
            0317, Norway"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6181
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        48..244
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     CDS             248..1207
                     /note="Aquaporin-4 from Rattus norvegicus. Accession#:
     CDS             1205..2854
                     /note="firefly luciferase"
     polyA_signal    complement(2898..3019)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3684..4272)
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
     CDS             complement(4446..5303)
     promoter        complement(5304..5408)
                     /label=AmpR promoter
     rep_origin      5435..5890
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    6021..6069
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    6083..6174
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"

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