Basic Vector Information
praAqp4-Luc vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
praAqp4-Luc vector Sequence
LOCUS 40924_36303 6181 bp DNA circular SYN 18-DEC-2018 DEFINITION Reporter vector praAqp4-Luc, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6181) AUTHORS Holen T, Moe SE, Sorbo JG, Meza TJ, Ottersen OP, Klungland A. TITLE Tolerated wobble mutations in siRNAs decrease specificity, but can enhance activity in vivo JOURNAL Nucleic Acids Res. 33 (15), 4704-4710 (2005) PUBMED 16113241 REFERENCE 2 (bases 1 to 6181) AUTHORS Holen T, Ottersen OP, Klungland A. TITLE Direct Submission JOURNAL Submitted (15-OCT-2004) CMBN, University of Oslo, Sognsvannsveien 9, Oslo 0317, Norway REFERENCE 3 (bases 1 to 6181) TITLE Direct Submission REFERENCE 4 (bases 1 to 6181) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic Acids Res."; date: "2005"; volume: "33"; issue: "15"; pages: "4704-4710" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (15-OCT-2004) CMBN, University of Oslo, Sognsvannsveien 9, Oslo 0317, Norway" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..6181 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 48..244 /label=SV40 promoter /note="SV40 early promoter" CDS 248..1207 /gene="Aqp4" /label=Aqp4 /note="Aquaporin-4 from Rattus norvegicus. Accession#: P47863" CDS 1205..2854 /label=luciferase /note="firefly luciferase" polyA_signal complement(2898..3019) /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" rep_origin complement(3684..4272) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(4446..5303) /label=AmpR /note="beta-lactamase" promoter complement(5304..5408) /label=AmpR promoter rep_origin 5435..5890 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" polyA_signal 6021..6069 /label=poly(A) signal /note="synthetic polyadenylation signal" misc_feature 6083..6174 /label=pause site /note="RNA polymerase II transcriptional pause signal from the human alpha-2 globin gene"
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