LOCUS       V003836                 6181 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003836
VERSION     V003836
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6181)
  AUTHORS   Holen T, Moe SE, Sorbo JG, Meza TJ, Ottersen OP, Klungland A.
  TITLE     Tolerated wobble mutations in siRNAs decrease specificity, but can
            enhance activity in vivo
  JOURNAL   Nucleic Acids Res. 33 (15), 4704-4710 (2005)
   PUBMED   16113241
REFERENCE   2  (bases 1 to 6181)
  AUTHORS   Holen T, Ottersen OP, Klungland A.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2004) CMBN, University of Oslo, Sognsvannsveien 9,
            Oslo 0317, Norway
REFERENCE   3  (bases 1 to 6181)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6181)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic
            Acids Res."; date: "2005"; volume: "33"; issue: "15"; pages:
            "4704-4710"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (15-OCT-2004) CMBN, University of Oslo, Sognsvannsveien 9, Oslo
            0317, Norway"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6181
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        48..244
                     /label="SV40 promoter"
                     /note="SV40 early promoter"
     CDS             248..1207
                     /gene="Aqp4"
                     /label="Aquaporin-4"
                     /note="Aquaporin-4 from Rattus norvegicus. Accession#:
                     P47863"
     CDS             1205..2854
                     /label="luciferase"
                     /note="firefly luciferase"
     polyA_signal    complement(2898..3019)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3684..4272)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4446..5303)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(5304..5408)
                     /label="AmpR promoter"
     rep_origin      5435..5890
                     /direction=RIGHT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    6021..6069
                     /label="poly(A) signal"
                     /note="synthetic polyadenylation signal"
     misc_feature    6083..6174
                     /label="pause site"
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggtaccgagc tcttacgcgt gctagcccgg gctcgagatc tgcgatctgc atctcaatta
       61 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
      121 cgcccattct ccgccccatc gctgactaat tttttttatt tatgcagagg ccgaggccgc
      181 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
      241 caaaaagctt agtgacggag ctgcagcgag gcggtggggt aagtgtggac ctccctgcag
      301 cagagagagc atcatggtgg ctttcaaagg cgtctggact caagccttct ggaaggcggt
      361 cacagcagag ttcctggcca tgctcatctt tgttctgctc agcgtgggat ccaccattaa
      421 ctggggtggc tcagagaacc ccctacctgt ggacatggtc ctcatctccc tctgctttgg
      481 actcagcatt gccaccatgg ttcagtgctt cggccacatc agcggtggcc acatcaaccc
      541 agcggtgaca gtggccatgg tgtgcacacg aaagatcagc atcgccaagt ctgtcttcta
      601 catcactgcg cagtgcctgg gggccatcat cggagctggg atcctctacc tggtcacacc
      661 ccccagcgtg gtgggaggat tgggagtcac cacggttcat ggaaacctca ctgctggcca
      721 tgggctcctg gtggagctaa taatcacttt ccagctggta ttcaccattt ttgccagctg
      781 tgattccaaa cggactgatg ttactggttc cgttgcttta gcaattgggt tttccgttgc
      841 aattggacat ttgtttgcaa tcaattatac cggagccagc atgaatccag ctcgatcctt
      901 tggccctgca gttatcatgg gaaactggga aaaccactgg atatattggg ttggaccaat
      961 cataggcgct gtgctggcag gtgcacttta cgagtatgtc ttctgtcctg acgtggagct
     1021 caaacgtcgc ctaaaggaag ccttcagcaa agctgcacag cagacgaaag ggagctacat
     1081 ggaggtggag gacaaccgga gccaagtgga gacagaagac ttgatcctga agcccggggt
     1141 ggtgcatgtg atcgacattg accgtggaga cgagaagaag gggaaggact cgtctggaga
     1201 ggtcatggaa gacgccaaaa acataaagaa aggcccggcg ccattctatc cgctggaaga
     1261 tggaaccgct ggagagcaac tgcataaggc tatgaagaga tacgccctgg ttcctggaac
     1321 aattgctttt acagatgcac atatcgaggt ggacatcact tacgctgagt acttcgaaat
     1381 gtccgttcgg ttggcagaag ctatgaaacg atatgggctg aatacaaatc acagaatcgt
     1441 cgtatgcagt gaaaactctc ttcaattctt tatgccggtg ttgggcgcgt tatttatcgg
     1501 agttgcagtt gcgcccgcga acgacattta taatgaacgt gaattgctca acagtatggg
     1561 catttcgcag cctaccgtgg tgttcgtttc caaaaagggg ttgcaaaaaa ttttgaacgt
     1621 gcaaaaaaag ctcccaatca tccaaaaaat tattatcatg gattctaaaa cggattacca
     1681 gggatttcag tcgatgtaca cgttcgtcac atctcatcta cctcccggtt ttaatgaata
     1741 cgattttgtg ccagagtcct tcgataggga caagacaatt gcactgatca tgaactcctc
     1801 tggatctact ggtctgccta aaggtgtcgc tctgcctcat agaactgcct gcgtgagatt
     1861 ctcgcatgcc agagatccta tttttggcaa tcaaatcatt ccggatactg cgattttaag
     1921 tgttgttcca ttccatcacg gttttggaat gtttactaca ctcggatatt tgatatgtgg
     1981 atttcgagtc gtcttaatgt atagatttga agaagagctg tttctgagga gccttcagga
     2041 ttacaagatt caaagtgcgc tgctggtgcc aaccctattc tccttcttcg ccaaaagcac
     2101 tctgattgac aaatacgatt tatctaattt acacgaaatt gcttctggtg gcgctcccct
     2161 ctctaaggaa gtcggggaag cggttgccaa gaggttccat ctgccaggta tcaggcaagg
     2221 atatgggctc actgagacta catcagctat tctgattaca cccgaggggg atgataaacc
     2281 gggcgcggtc ggtaaagttg ttccattttt tgaagcgaag gttgtggatc tggataccgg
     2341 gaaaacgctg ggcgttaatc aaagaggcga actgtgtgtg agaggtccta tgattatgtc
     2401 cggttatgta aacaatccgg aagcgaccaa cgccttgatt gacaaggatg gatggctaca
     2461 ttctggagac atagcttact gggacgaaga cgaacacttc ttcatcgttg accgcctgaa
     2521 gtctctgatt aagtacaaag gctatcaggt ggctcccgct gaattggaat ccatcttgct
     2581 ccaacacccc aacatcttcg acgcaggtgt cgcaggtctt cccgacgatg acgccggtga
     2641 acttcccgcc gccgttgttg ttttggagca cggaaagacg atgacggaaa aagagatcgt
     2701 ggattacgtc gccagtcaag taacaaccgc gaaaaagttg cgcggaggag ttgtgtttgt
     2761 ggacgaagta ccgaaaggtc ttaccggaaa actcgacgca agaaaaatca gagagatcct
     2821 cataaaggcc aagaagggcg gaaagatcgc cgtgtaattc tagagtcggg gcggccggcc
     2881 gcttcgagca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca
     2941 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
     3001 aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg
     3061 ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta aaatcgataa
     3121 ggatctgaac gatggagcgg agaatgggcg gaactgggcg gagttagggg cgggatgggc
     3181 ggagttaggg gcgggactat ggttgctgac taattgagat gcatgctttg catacttctg
     3241 cctgctgggg agcctgggga ctttccacac ctggttgctg actaattgag atgcatgctt
     3301 tgcatacttc tgcctgctgg ggagcctggg gactttccac accctaactg acacacattc
     3361 cacagcggat ccgtcgaccg atgcccttga gagccttcaa cccagtcagc tccttccggt
     3421 gggcgcgggg catgactatc gtcgccgcac ttatgactgt cttctttatc atgcaactcg
     3481 taggacaggt gccggcagcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     3541 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     3601 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     3661 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     3721 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     3781 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     3841 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     3901 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     3961 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     4021 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     4081 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     4141 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     4201 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     4261 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     4321 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     4381 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     4441 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     4501 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     4561 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     4621 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     4681 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     4741 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     4801 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     4861 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     4921 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     4981 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     5041 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     5101 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     5161 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     5221 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     5281 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     5341 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     5401 gttccgcgca catttccccg aaaagtgcca cctgacgcgc cctgtagcgg cgcattaagc
     5461 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     5521 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     5581 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     5641 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     5701 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     5761 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     5821 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     5881 cttacaattt gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg
     5941 gcctcttcgc tattacgcca gcccaagcta ccatgataag taagtaatat taaggtacgg
     6001 gaggtacttg gagcggccgc aataaaatat ctttattttc attacatctg tgtgttggtt
     6061 ttttgtgtga atcgatagta ctaacatacg ctctccatca aaacaaaacg aaacaaaaca
     6121 aactagcaaa ataggctgtc cccagtgcaa gtgcaggtgc cagaacattt ctctatcgat
     6181 a
//
