pR18GFP vector (V003841)

LOCUS       40924_36278        3655 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pR18GFP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3655)
  AUTHORS   Cheng M, Zhang H, Guo S, He J, Li S, Huang X.
  TITLE     Screening Transcription Factor Genes Involved in Bacterial 
            Xenobiotic Catabolism using Modified SIGEX Approach
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3655)
  AUTHORS   Cheng M, Zhang H, Guo S, He J, Li S, Huang X.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2015) Department of Microbiology, College of Life 
            Sciences, Nanjing Agricultural University, Tongwei Road #6, Nanjing,
            Jiangsu 210095, China
REFERENCE   3  (bases 1 to 3655)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3655)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (21-JAN-2015) Department of Microbiology, College of Life Sciences, 
            Nanjing Agricultural University, Tongwei Road #6, Nanjing, Jiangsu 
            210095, China"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3655
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      47..94
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     regulatory      306..311
                     /regulatory_class="ribosome_binding_site"
     CDS             319..1035
                     /label=EGFP
                     /note="enhanced GFP"
     primer_bind     1418..1434
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(1438..1494)
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(1504..1520)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1528..1544)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1552..1582)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1597..1618)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1906..2494)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2668..3525)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3526..3630)
                     /label=AmpR promoter