LOCUS       40924_36278        3655 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pR18GFP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3655)
  AUTHORS   Cheng M, Zhang H, Guo S, He J, Li S, Huang X.
  TITLE     Screening Transcription Factor Genes Involved in Bacterial 
            Xenobiotic Catabolism using Modified SIGEX Approach
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3655)
  AUTHORS   Cheng M, Zhang H, Guo S, He J, Li S, Huang X.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2015) Department of Microbiology, College of Life 
            Sciences, Nanjing Agricultural University, Tongwei Road #6, Nanjing,
            Jiangsu 210095, China
REFERENCE   3  (bases 1 to 3655)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3655)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (21-JAN-2015) Department of Microbiology, College of Life Sciences, 
            Nanjing Agricultural University, Tongwei Road #6, Nanjing, Jiangsu 
            210095, China"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3655
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      47..94
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     regulatory      306..311
                     /regulatory_class="ribosome_binding_site"
     CDS             319..1035
                     /label=EGFP
                     /note="enhanced GFP"
     primer_bind     1418..1434
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(1438..1494)
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(1504..1520)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1528..1544)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1552..1582)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1597..1618)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1906..2494)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2668..3525)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3526..3630)
                     /label=AmpR promoter
ORIGIN
        1 gacgtcaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct
       61 tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggatt
      121 ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
      181 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttccctcga
      241 gatctgatat cctaggttaa caattgctag ccatggatgc atacgtacta gttaattaat
      301 taagaaggag atatacatat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
      361 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
      421 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
      481 cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc
      541 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
      601 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
      661 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
      721 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg
      781 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac
      841 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
      901 ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag
      961 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
     1021 gacgagctgt acaagtaaac gcgtcgtttc ggtgatgacg gtgaaaacct ctgacacatg
     1081 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
     1141 cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag
     1201 cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga
     1261 aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg
     1321 gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta
     1381 agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgccaag
     1441 cttgcatgcc tgcaggtcga ctctagagga tccccgggta ccgagctcga attcgtaatc
     1501 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     1561 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     1621 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     1681 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     1741 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     1801 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     1861 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     1921 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     1981 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     2041 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     2101 aagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     2161 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     2221 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     2281 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     2341 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     2401 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     2461 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     2521 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     2581 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     2641 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     2701 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     2761 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     2821 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     2881 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     2941 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     3001 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     3061 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     3121 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     3181 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     3241 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     3301 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     3361 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     3421 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     3481 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     3541 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     3601 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacct
//
