pQS-GFP vector (V003848)

LOCUS       V003848                 5736 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003848
VERSION     V003848
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5736)
  AUTHORS   Swofford CA, Van Dessel N, Forbes NS.
  TITLE     Quorum-sensing Salmonella selectively trigger protein expression
            within tumors
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 112 (11), 3457-3462 (2015)
   PUBMED   25737556
REFERENCE   2  (bases 1 to 5736)
  AUTHORS   Swofford CA, Van Dessel N, Forbes NS.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2014) Chemical Engineering, University of
            Massachusetts Amherst, 686 North Pleasant Street, Amherst, MA
            01003-9303, USA
REFERENCE   3  (bases 1 to 5736)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5736)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Proc. Natl.
            Acad. Sci. U.S.A."; date: "2015"; volume: "112"; issue: "11"; pages:
            "3457-3462"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (18-DEC-2014) Chemical Engineering, University of Massachusetts
            Amherst, 686 North Pleasant Street, Amherst, MA 01003-9303, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5736
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(28..616)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             961..2064
                     /gene="asd"
                     /label="Aspartate-semialdehyde dehydrogenase"
                     /note="Aspartate-semialdehyde dehydrogenase from Salmonella
                     typhimurium (strain LT2 / SGSC1412 / ATCC 700720).
                     Accession#: P0A1F8"
     terminator      complement(2225..2319)
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     CDS             complement(2343..2999)
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(3000..3102)
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     regulatory      3132..3276
                     /label="luxI"
                     /note="luxI"
                     /regulatory_class="promoter"
     primer_bind     3288..3304
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             3332..4045
                     /label="yeGFP"
                     /note="yeast-enhanced green fluorescent protein"
     RBS             4132..4143
                     /note="strong bacterial ribosome binding site (Elowitz and
                     Leibler, 2000)"
     CDS             4151..4900
                     /gene="luxR"
                     /label="Transcriptional activator protein LuxR"
                     /note="Transcriptional activator protein LuxR from
                     Aliivibrio fischeri. Accession#: P12746"
     CDS             4934..5512
                     /gene="luxI"
                     /label="Acyl-homoserine-lactone synthase"
                     /note="Acyl-homoserine-lactone synthase from Aliivibrio
                     fischeri. Accession#: P12747"