LOCUS       V003848                 5736 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003848
VERSION     V003848
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5736)
  AUTHORS   Swofford CA, Van Dessel N, Forbes NS.
  TITLE     Quorum-sensing Salmonella selectively trigger protein expression
            within tumors
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 112 (11), 3457-3462 (2015)
   PUBMED   25737556
REFERENCE   2  (bases 1 to 5736)
  AUTHORS   Swofford CA, Van Dessel N, Forbes NS.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2014) Chemical Engineering, University of
            Massachusetts Amherst, 686 North Pleasant Street, Amherst, MA
            01003-9303, USA
REFERENCE   3  (bases 1 to 5736)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5736)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Proc. Natl.
            Acad. Sci. U.S.A."; date: "2015"; volume: "112"; issue: "11"; pages:
            "3457-3462"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (18-DEC-2014) Chemical Engineering, University of Massachusetts
            Amherst, 686 North Pleasant Street, Amherst, MA 01003-9303, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5736
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(28..616)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             961..2064
                     /gene="asd"
                     /label="Aspartate-semialdehyde dehydrogenase"
                     /note="Aspartate-semialdehyde dehydrogenase from Salmonella
                     typhimurium (strain LT2 / SGSC1412 / ATCC 700720).
                     Accession#: P0A1F8"
     terminator      complement(2225..2319)
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     CDS             complement(2343..2999)
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(3000..3102)
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     regulatory      3132..3276
                     /label="luxI"
                     /note="luxI"
                     /regulatory_class="promoter"
     primer_bind     3288..3304
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             3332..4045
                     /label="yeGFP"
                     /note="yeast-enhanced green fluorescent protein"
     RBS             4132..4143
                     /note="strong bacterial ribosome binding site (Elowitz and
                     Leibler, 2000)"
     CDS             4151..4900
                     /gene="luxR"
                     /label="Transcriptional activator protein LuxR"
                     /note="Transcriptional activator protein LuxR from
                     Aliivibrio fischeri. Accession#: P12746"
     CDS             4934..5512
                     /gene="luxI"
                     /label="Acyl-homoserine-lactone synthase"
                     /note="Acyl-homoserine-lactone synthase from Aliivibrio
                     fischeri. Accession#: P12747"
ORIGIN
        1 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
       61 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
      121 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
      181 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct caatgctcac gctgtaggta
      241 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
      301 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
      361 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
      421 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
      481 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
      541 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
      601 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
      661 cgaaaactca cgttaaggga ttttggtcat gacgtacgtt ttcgttccat tggccctcaa
      721 acccctaatt aggatcaata aaacagcgac ggaaatgatt cccttcctaa cgcaaattcc
      781 ctgataatcg ccactggact ttctgcttgc gcggtaaggc aggataagtc gcattactga
      841 tggcttcgct atcattgatt aatttcactt gcgactttgg ctgctttttg tatggtgaag
      901 gatgcgccac aggatactgg cgcgcataca cagcacatct ctttgcagga aaaaaacgct
      961 atgaaaaatg ttggttttat cggctggcgc ggaatggtcg gctctgttct catgcaacgc
     1021 atggtagagg agcgcgattt cgacgctatt cgccctgttt tcttttctac ctcccagttt
     1081 ggacaggcgg cgcccacctt cggcgacacc tccaccggca cgctacagga cgcttttgat
     1141 ctggatgcgc taaaagcgct cgatatcatc gtgacctgcc agggcggcga ttataccaac
     1201 gaaatttatc caaagctgcg cgaaagcgga tggcagggtt actggattga tgcggcttct
     1261 acgctgcgca tgaaagatga tgccattatt attctcgacc cggtcaacca ggacgtgatt
     1321 accgacggcc tgaacaatgg cgtgaagacc tttgtgggcg gtaactgtac cgttagcctg
     1381 atgttgatgt cgctgggcgg tctctttgcc cataatctcg ttgactgggt atccgtcgcg
     1441 acctatcagg ccgcctccgg cggcggcgcg cgccatatgc gcgagctgtt aacccagatg
     1501 ggtcagttgt atggccatgt cgccgatgaa ctggcgacgc cgtcttccgc aattcttgat
     1561 attgaacgca aagttacggc attgacccgc agcggcgagc tgccggttga taactttggc
     1621 gtaccgctgg cgggaagcct gatcccctgg atcgacaaac agctcgataa cggccagagc
     1681 cgcgaagagt ggaaaggcca ggcggaaacc aacaagattc tcaatactgc ctctgtgatt
     1741 ccggttgatg gtttgtgtgt gcgcgtcggc gcgctgcgct gtcacagcca ggcgttcacc
     1801 atcaagctga aaaaagaggt atccattccg acggtggaag aactgctggc ggcacataat
     1861 ccgtgggcga aagtggtgcc gaacgatcgt gatatcacta tgcgcgaatt aaccccggcg
     1921 gcggtgaccg gcacgttgac tacgccggtt ggtcgtctgc gtaagctgaa catggggcca
     1981 gagttcttgt cggcgtttac cgtaggcgac cagttgttat ggggcgccgc cgagccgctg
     2041 cgtcgaatgc tgcgccagtt ggcgtagtgg ctattgcagc gcttatcggg cctgcgtgtg
     2101 gttctgtagg ccggataagg cgcgtcagcg ccgccatccg gcggggaaat ttgtgttaaa
     2161 ccaggggtgc atcgtcaccc tttttttgcg taatacagga gtaaacgcag atactagtgc
     2221 ttggattctc accaataaaa aacgcccggc ggcaaccgag cgttctgaac aaatccagat
     2281 ggagttctga ggtcattact ggatctatca acaggagtcc aagcgagctc gatatcaaat
     2341 tacgccccgc cctgccactc atcgcagtac tgttgtaatt cattaagcat tctgccgaca
     2401 tggaagccat cacagacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg
     2461 ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc
     2521 acgtttaaat caaaactggt gaaactcacc cagggattgg ctgagacgaa aaacatattc
     2581 tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa
     2641 tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt
     2701 tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca
     2761 ccgtctttca ttgccatacg aaattccgga tgagcattca tcaggcgggc aagaatgtga
     2821 ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata
     2881 tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt
     2941 tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt
     3001 ttagcttcct tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt
     3061 atttcattat ggtgaaagtt ggaacctctt acgtgccgat caacgtctca ttttcgccag
     3121 atatcgacgt cagtcctttg attctaataa attggatttt tgtcacacta ttgtatcgct
     3181 gggaatacaa ttacttaaca taagcacctg taggatcgta caggtttacg caagaaaatg
     3241 gtttgttata gtcgaataaa cgcaagggag gttggtgcta gctcacacag gaaacagcta
     3301 tgacctctag atttaagaag gagatataca tatgagtaaa ggagaagaac ttttcactgg
     3361 agttgtccca attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtcag
     3421 tggagagggt gaaggtgatg caacatacgg aaaacttacc cttaaattta tttgcactac
     3481 tggaaaacta cctgttccat ggccaacact tgtcactact ttcgggtatg gtgttcaatg
     3541 ctttgcgaga tacccagatc atatgaaaca gcatgacttt ttcaagagtg ccatgcccga
     3601 aggttatgta caggaaagaa ctatattttt caaagatgac gggaactaca agacacgtgc
     3661 tgaagtcaag tttgaaggtg atacccttgt taatagaatc gagttaaaag gtattgattt
     3721 taaagaagat ggaaacattc ttggacacaa attggaatac aactataact cacacaatgt
     3781 atacatcatg gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa
     3841 cattgaagat ggaagcgttc aactagcaga ccattatcaa caaaatactc caattggcga
     3901 tggccctgtc cttttaccag acaaccatta cctgtccaca caatctgccc tttcgaaaga
     3961 tcccaacgaa aagagagacc acatggtcct tcttgagttt gtaacagctg ctgggattac
     4021 acatggcatg gatgaactat acaaataaat ctcctgcagg catgcaagct taatgcggta
     4081 gtttatcaca gttaaattgc taacgcagtc aggcaccgtg tatcgatcgt taaagaggag
     4141 aaaggtaccc atgaaaaaca taaatgccga cgacacatac agaataatta ataaaattaa
     4201 agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta aaatggtaca
     4261 ttgtgaatat tatttactcg cgatcattta tcctcattct atggttaaat ctgatatttc
     4321 aattctagat aattacccta aaaaatggag gcaatattat gatgacgcta atttaataaa
     4381 atatgatcct atagtagatt attctaactc caatcattca ccaattaatt ggaatatatt
     4441 tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga aaacatcagg
     4501 tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg gaatgcttag
     4561 ttttgcacat tcagaaaaag acaactatat agatagttta tttttacatg cgtgtatgaa
     4621 cataccatta attgttcctt ctctagttga taattatcga aaaataaata tagcaaataa
     4681 taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg catgcgaagg
     4741 aaaaagctct tgggatattt caaaaatatt aggctgcagt gagcgtactg tcactttcca
     4801 tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta tttctaaagc
     4861 aattttaaca ggagcaattg attgcccata ctttaaaaat taagcggccg gaataaacgc
     4921 aagggaggtt ggtatgacta taatgataaa aaaatcggat tttttggcaa ttccatcgga
     4981 ggagtataaa ggtattctaa gtcttcgtta tcaagtgttt aagcaaagac ttgagtggga
     5041 cttagttgta gaaaataacc ttgaatcaga tgagtatgat aactcaaatg cagaatatat
     5101 ttatgcttgt gatgatactg aaaatgtaag tggatgctgg cgtttattac ctacaacagg
     5161 tgattatatg ctgaaaagtg tttttcctga attgcttggt caacagagtg ctcccaaaga
     5221 tcctaatata gtcgaattaa gtcgttttgc tgtaggtaaa aatagctcaa agataaataa
     5281 ctctgctagt gaaattacaa tgaaactatt tgaagctata tataaacacg ctgttagtca
     5341 aggtattaca gaatatgtaa cagtaacatc aacagcaata gagcgatttt taaagcgtat
     5401 taaagttcct tgtcatcgta ttggagacaa agaaattcat gtattaggtg atactaaatc
     5461 ggttgtattg tctatgccta ttaatgaaca gtttaaaaaa gcagtcttaa attaatgttg
     5521 ttaaatcatt aatttatttt aaatactaag tatattatag ggaaaataat gaataaatgt
     5581 attccaatga taattaatgg aatgattcaa gatcctaggc gttcggctgc ggcgagcggt
     5641 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     5701 gaacatgtga gcaaaaggcc agcaaaaggc caggaa
//
