pQE-30NAST-attB vector (V003858)

LOCUS       40924_36018        3575 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pQE-30NAST-attB, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3575)
  AUTHORS   Kersten B, Feilner T, Kramer A, Wehrmeyer S, Possling A, Witt I, 
            Zanor MI, Stracke R, Lueking A, Kreutzberger J, Lehrach H, Cahilll 
            DJ.
  TITLE     Generation of Arabidopsis protein chips for antibody and serum 
            screening
  JOURNAL   Plant Mol. Biol. 52 (5), 999-1010 (2003)
  PUBMED    14558660
REFERENCE   2  (bases 1 to 3575)
  AUTHORS   Kersten B, Feilner T, Kramer A, Wehrmeyer S, Possling A, Witt I, 
            Zanor MI, Stracke R, Lueking A, Kreutzberger J, Lehrach H, Cahill 
            DJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2003) Max-Planck Institute for Molecular Genetics,
            Ihnestrasse 73, Berlin 14195, Germany
REFERENCE   3  (bases 1 to 3575)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3575)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Mol. 
            Biol."; date: "2003"; volume: "52"; issue: "5"; pages: "999-1010"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (09-SEP-2003) Max-Planck Institute for Molecular Genetics, 
            Ihnestrasse 73, Berlin 14195, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3575
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..54
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA
                     polymerase, with embedded lac operator"
     protein_bind    62..78
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             97..108
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     regulatory      101..107
                     /regulatory_class="ribosome_binding_site"
     misc_feature    115
                     /label=translation start site for inserts
                     /note="translation start site for inserts"
     CDS             127..144
                     /label=6xHis
                     /note="6xHis affinity tag"
     promoter        152..170
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     protein_bind    181..205
                     /label=attB1
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    207..240
                     /note="multiple cloning site for SalI, HincII, BssHII,
                     AscI, and NotI"
     protein_bind    complement(242..266)
                     /label=attB2
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(282..300)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      321..415
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             459..1115
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     terminator      1183..1269
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     misc_feature    1425..1565
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(1751..2339)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2513..3370)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3371..3475)
                     /label=AmpR promoter