LOCUS       40924_36018        3575 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pQE-30NAST-attB, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3575)
  AUTHORS   Kersten B, Feilner T, Kramer A, Wehrmeyer S, Possling A, Witt I, 
            Zanor MI, Stracke R, Lueking A, Kreutzberger J, Lehrach H, Cahilll 
            DJ.
  TITLE     Generation of Arabidopsis protein chips for antibody and serum 
            screening
  JOURNAL   Plant Mol. Biol. 52 (5), 999-1010 (2003)
  PUBMED    14558660
REFERENCE   2  (bases 1 to 3575)
  AUTHORS   Kersten B, Feilner T, Kramer A, Wehrmeyer S, Possling A, Witt I, 
            Zanor MI, Stracke R, Lueking A, Kreutzberger J, Lehrach H, Cahill 
            DJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2003) Max-Planck Institute for Molecular Genetics,
            Ihnestrasse 73, Berlin 14195, Germany
REFERENCE   3  (bases 1 to 3575)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3575)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Mol. 
            Biol."; date: "2003"; volume: "52"; issue: "5"; pages: "999-1010"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (09-SEP-2003) Max-Planck Institute for Molecular Genetics, 
            Ihnestrasse 73, Berlin 14195, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3575
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..54
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA
                     polymerase, with embedded lac operator"
     protein_bind    62..78
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             97..108
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     regulatory      101..107
                     /regulatory_class="ribosome_binding_site"
     misc_feature    115
                     /label=translation start site for inserts
                     /note="translation start site for inserts"
     CDS             127..144
                     /label=6xHis
                     /note="6xHis affinity tag"
     promoter        152..170
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     protein_bind    181..205
                     /label=attB1
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    207..240
                     /note="multiple cloning site for SalI, HincII, BssHII,
                     AscI, and NotI"
     protein_bind    complement(242..266)
                     /label=attB2
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(282..300)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      321..415
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             459..1115
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     terminator      1183..1269
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     misc_feature    1425..1565
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(1751..2339)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2513..3370)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3371..3475)
                     /label=AmpR promoter
ORIGIN
        1 ctcgagaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat aatagattca
       61 attgtgagcg gataacaatt tcacacagaa ttcattaaag aggagaaatt aactatgaga
      121 ggatcgcatc accatcacca tcacggatcc tatttaggtg acactataga atcgtcgagc
      181 acaagtttgt acaaaaaagc aggcttgtcg accaaggcgc gcctgctggg tggcggccgc
      241 aacccagctt tcttgtacaa agtggtttaa accggccgct ccctatagtg agtcgtatta
      301 agcttaatta gctgagcttg gactcctgtt gatagatcca gtaatgacct cagaactcca
      361 tctggatttg ttcagaacgc tcggttgccg ccgggcgttt tttattggtg agaatccaag
      421 ctagcttggc gagattttca ggagctaagg aagctaaaat ggagaaaaaa atcactggat
      481 ataccaccgt tgatatatcc caatggcatc gtaaagaaca ttttgaggca tttcagtcag
      541 ttgctcaatg tacctataac cagaccgttc agctggatat tacggccttt ttaaagaccg
      601 taaagaaaaa taagcacaag ttttatccgg cctttattca cattcttgcc cgcctgatga
      661 atgctcatcc ggaatttcgt atggcaatga aagacggtga gctggtgata tgggatagtg
      721 ttcacccttg ttacaccgtt ttccatgagc aaactgaaac gttttcatcg ctctggagtg
      781 aataccacga cgatttccgg cagtttctac acatatattc gcaagatgtg gcgtgttacg
      841 gtgaaaacct ggcctatttc cctaaagggt ttattgagaa tatgtttttc gtctcagcca
      901 atccctgggt gagtttcacc agttttgatt taaacgtggc caatatggac aacttcttcg
      961 cccccgtttt caccatgggc aaatattata cgcaaggcga caaggtgctg atgccgctgg
     1021 cgattcaggt tcatcatgcc gtctgtgatg gcttccatgt cggcagaatg cttaatgaat
     1081 tacaacagta ctgcgatgag tggcagggcg gggcgtaatt tttttaaggc agttattggt
     1141 gcccttaaac gcctggggta atgactctct agcttgaggc atcaaataaa acgaaaggct
     1201 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt
     1261 aggacaaatc cgccgctcta gagctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc
     1321 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
     1381 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag
     1441 tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag cagattgtac
     1501 tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
     1561 tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtctgt cggctgcggc
     1621 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     1681 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     1741 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     1801 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     1861 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     1921 cttcgggaag cgtggcgctt tctcaatgct cacgctgtag gtatctcagt tcggtgtagg
     1981 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     2041 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     2101 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     2161 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
     2221 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     2281 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     2341 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     2401 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     2461 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     2521 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gctgcctgac
     2581 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     2641 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     2701 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     2761 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     2821 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     2881 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     2941 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     3001 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     3061 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     3121 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     3181 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     3241 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     3301 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     3361 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     3421 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     3481 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
     3541 aaaataggcg tatcacgagg ccctttcgtc ttcac
//
