pQC2319 vector (V003859)

LOCUS       40924_35903        7015 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pQC2319, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7015)
  AUTHORS   Chen Q, Lee G, Craig C, Ng V, Carlson PE Jr., Hinton DM, Stibitz S.
  TITLE     A novel Bvg-repressed promoter causes vrg-like transcription of fim3
            but does not result in the production of serotype 3 Fimbriae in the 
            Bvg(-) mode Bordetella pertussis
  JOURNAL   J. Bacteriol. (2018) In press
  PUBMED    30061354
REFERENCE   2  (bases 1 to 7015)
  AUTHORS   Chen Q, Lee G, Craig C, Ng V, Carlson P, Hinton D, Stibitz S.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-JUL-2018) Division of Bacterial, Parasitic, and 
            Allergenic Products, Center for Biologics Evaluation and Research, 
            FDA, 10903 New Hampshire Avenue, Silver Spring, MD 20933, USA
REFERENCE   3  (bases 1 to 7015)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7015)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. 
            Bacteriol. (2018) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (05-JUL-2018) Division of Bacterial, Parasitic, and Allergenic 
            Products, Center for Biologics Evaluation and Research, FDA, 10903 
            New Hampshire Avenue, Silver Spring, MD 20933, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7015
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      58..144
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             complement(224..916)
                     /codon_start=1
                     /label=TurboRFP
                     /note="red fluorescent protein from Entacmaea quadricolor"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV
                     VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA
                     TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK
                     LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC
                     DLPSKLGHR"
     terminator      1291..1334
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      complement(1434..1520)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     promoter        2288..2316
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             2505..3035
                     /codon_start=1
                     /label=GmR
                     /note="gentamycin acetyltransferase"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     oriT            complement(3057..3165)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             4587..4775
                     /codon_start=1
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
                     /translation="VTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     misc_feature    4880..5020
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(5206..5794)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5968..6825)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6826..6930)
                     /label=AmpR promoter