LOCUS       40924_35903        7015 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pQC2319, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7015)
  AUTHORS   Chen Q, Lee G, Craig C, Ng V, Carlson PE Jr., Hinton DM, Stibitz S.
  TITLE     A novel Bvg-repressed promoter causes vrg-like transcription of fim3
            but does not result in the production of serotype 3 Fimbriae in the 
            Bvg(-) mode Bordetella pertussis
  JOURNAL   J. Bacteriol. (2018) In press
  PUBMED    30061354
REFERENCE   2  (bases 1 to 7015)
  AUTHORS   Chen Q, Lee G, Craig C, Ng V, Carlson P, Hinton D, Stibitz S.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-JUL-2018) Division of Bacterial, Parasitic, and 
            Allergenic Products, Center for Biologics Evaluation and Research, 
            FDA, 10903 New Hampshire Avenue, Silver Spring, MD 20933, USA
REFERENCE   3  (bases 1 to 7015)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7015)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. 
            Bacteriol. (2018) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (05-JUL-2018) Division of Bacterial, Parasitic, and Allergenic 
            Products, Center for Biologics Evaluation and Research, FDA, 10903 
            New Hampshire Avenue, Silver Spring, MD 20933, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7015
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      58..144
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             complement(224..916)
                     /codon_start=1
                     /label=TurboRFP
                     /note="red fluorescent protein from Entacmaea quadricolor"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV
                     VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA
                     TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK
                     LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC
                     DLPSKLGHR"
     terminator      1291..1334
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      complement(1434..1520)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     promoter        2288..2316
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             2505..3035
                     /codon_start=1
                     /label=GmR
                     /note="gentamycin acetyltransferase"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     oriT            complement(3057..3165)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             4587..4775
                     /codon_start=1
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
                     /translation="VTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     misc_feature    4880..5020
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(5206..5794)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5968..6825)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6826..6930)
                     /label=AmpR promoter
ORIGIN
        1 ccctttcgtc ttcaagaatt gcggccgccc atgcgagagt agggaactgc caggcatcaa
       61 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
      121 aacgctctcc tgagtaggac aaatccgccg ggagcggatt tgaacgttgc gaagcaacgg
      181 cccggagggt ggcgggcagg acgcccgccg cggatccagc ttatctatgt cctaattttg
      241 aaggtaaatc acaatatttt gctactgcca tttcatgttg ttcaacatat gtttctttat
      301 cagcttcttt aattctttct aaacgatgat ctacaaaatg aaatccaggc atttttaaat
      361 tttttgctgg ttttttagat ctatatgttg ttttaaaact acaatgtaaa tatcctccac
      421 ctactaattt taatgccatt tgagaatgtc ctcttaaacc tccatcagct ggatataaca
      481 tttctgtatt tgcttcccaa ccacgtgttt tcttttgcat aacaggtcca ttacttggaa
      541 aattaacacc attaattttt acattataaa taatacatcc attttgaaat gatgtatctt
      601 gtgtagctgt taatacacct ccatcttcat atgttgtaat acgttcccat gtaaaacctt
      661 caggaaatga ttgtttaaag aaatctggaa taccttgtgt atgattaata aatgcttttg
      721 aaccatacat aaaagatgta gctaaaatat caaatgcaaa aggtaatggt ccaccttcaa
      781 ctactttaat tttcattgtt tgtgtacctt catatggttt tccttcacct tcagatgtac
      841 atttaaaatg atgattattt actgtacctt ccatatataa tttcatatgc atattttctt
      901 taattaattc tgacatacta ccactacctc tcatgataag aactccttcg tcgacggatc
      961 cgaattctta attaacaatt ctctagacaa ttccaattcg taattggagt agaactgaaa
     1021 tcgtcttgtg catttaatgc gattggcgcc aagtttttag aaggataata attattaggt
     1081 gcaagtattt gacttaattg gttttcctta gctttaattg tcgcgagtca attcttaatt
     1141 aacaattctc tagacaattc cgatccccaa ttcctggcag tttatggcgg gcgtcctgcc
     1201 cgccaccctc cgggccgttg cttcgcaacg ttcaaatccg ctcccggcgg atttgtccta
     1261 ctcaggagag cgttcaccga caaacaacag ataaaacgaa aggcccagtc tttcgactga
     1321 gcctttcgtt ttatttgatg cctggggaat taattcctgg cagtttatgg cgggcgtcct
     1381 gcccgccacc ctccgggccg ttgcttcgca acgttcaaat ccgctcccgg cggatttgtc
     1441 ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtctttcgac
     1501 tgagcctttc gttttatttg atgcctgggg aattgggaat tgatcgctgt aatccgggca
     1561 gcgcaacgga acattcatca gtgtaaaaat ggaatcaata aagccctgcg cagcgcgcag
     1621 ggtcagcctg aatacgcgtt taatgaccag cacagtcgtg atggcaaggt cagaatagcg
     1681 ctgaggtctg cctcgtgaag aaggtgttgc tgactcatac caggcctgaa tcgccccatc
     1741 atccagccag aaagtgaggg agccacggtt gatgagagct ttgttgtagg tggaccagtt
     1801 ggtgattttg aacttttgct ttgccacgga acggtctgcg ttgtcgggaa gatgcgtgat
     1861 ctgatccttc aactcagcaa aagttcgatt tattcaacaa agccgccgtc ccgtcaagtc
     1921 agcgtaatgc tctgccagtg ttacaaccaa ttaaccaatt ctgattagaa aaactcatcg
     1981 agcatcaaat gaaactgcaa tttattcata tcaggattat caataccata tttttgaaaa
     2041 agccgtttct gtaatgaagg agaaaactca ccgaggcagt tccataggat ggcaagatcc
     2101 tggtatcggt ctgcgattcc gactcgtcca acatcaatac aacctattaa tttcccctcg
     2161 tcaaaaataa ggttatcaag tgagaaatca ccatgagtga cgactgaatc cggtgagaat
     2221 ggcaaaagct gcggccgttt aaacggccgc aattccaccg tggaaacgga tgaaggcacg
     2281 aacccagttg acataagcct gttcggttcg taaactgtaa tgcaagtagc gtatgcgctc
     2341 acgcaactgg tccagaacct tgaccgaacg cagcggtggt aacggcgcag tggcggtttt
     2401 catggcttgt tatgactgtt tttttgtaca gtctatgcct cgggcatcca agcagcaagc
     2461 gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc aacgatgtta cgcagcagca
     2521 acgatgttac gcagcagggc agtcgcccta aaacaaagtt aggtggctca agtatgggca
     2581 tcattcgcac atgtaggctc ggccctgacc aagtcaaatc catgcgggct gctcttgatc
     2641 ttttcggtcg tgagttcgga gacgtagcca cctactccca acatcagccg gactccgatt
     2701 acctcgggaa cttgctccgt agtaagacat tcatcgcgct tgctgccttc gaccaagaag
     2761 cggttgttgg cgctctcgcg gcttacgttc tgcccaggtt tgagcagccg cgtagtgaga
     2821 tctatatcta tgatctcgca gtctccggcg agcaccggag gcagggcatt gccaccgcgc
     2881 tcatcaatct cctcaagcat gaggccaacg cgcttggtgc ttatgtgatc tacgtgcaag
     2941 cagattacgg tgacgatccc gcagtggctc tctatacaaa gttgggcata cgggaagaag
     3001 tgatgcactt tgatatcgac ccaagtaccg ccacctaaaa gctgtttaaa cagcttcggc
     3061 cagcctcgca gagcaggatt cccgttgagc accgccaggt gcgaataagg gacagtgaag
     3121 aaggaacacc cgctcgcggg tgggcctact tcacctatcc tgcccggctg acgccgttgg
     3181 atacaccaag gaaagtctac acgaaccctt tggcaaaatc ctgtatatcg tgcgaaaaag
     3241 gatggatata ccgaaaaaat cgctataatg accccgaagc agggttatgc agcggaaaag
     3301 cgcgatccgt cgagatttaa atctcgaccg atgcccttga gagccttcaa cccagtcagc
     3361 tccttccggt gggcgcgggg catgactatc gtcgccgcac ttatgactgt cttctttatc
     3421 atgcaactcg taggacaggt gccggcagcg ctctgggtca ttttcggcga ggaccgcttt
     3481 cgctggagcg cgacgatgat cggcctgtcg cttgcggtat tcggaatctt gcacgccctc
     3541 gctcaagcct tcgtcactgg tcccgccacc aaacgtttcg gcgagaagca ggccattatc
     3601 gccggcatgg cggccgacgc gctgggctac gtcttgctgg cgttcgcgac gcgaggctgg
     3661 atggccttcc ccattatgat tcttctcgct tccggcggca tcgggatgcc cgcgttgcag
     3721 gccatgctgt ccaggcaggt agatgacgac catcagggac agcttcaagg atcgctcgcg
     3781 gctcttacca gcctaacttc gatcactgga ccgctgatcg tcacggcgat ttatgccgcc
     3841 tcggcgagca catggaacgg gttggcatgg attgtaggcg ccgccctata ccttgtctgc
     3901 ctccccgcgt tgcgtcgcgg tgcatggagc cgggccacct cgacctgaat ggaagccggc
     3961 ggcacctcgc taacggattc accactccaa gaattggagc caatcaattc ttgcggagaa
     4021 ctgtgaatgc gcaaaccaac ccttggcaga acatatccat cgcgtccgcc atctccagca
     4081 gccgcacgcg gcgcatctcg ggcagcgttg ggtcctggcc acgggtgcgc atgatcgtgc
     4141 tcctgtcgtt gaggacccgg ctaggctggc ggggttgcct tactggttag cagaatgaat
     4201 caccgatacg cgagcgaacg tgaagcgact gctgctgcaa aacgtctgcg acctgagcaa
     4261 caacatgaat ggtcttcggt ttccgtgttt cgtaaagtct ggaaacgcgg aagtcagcgc
     4321 cctgcaccat tatgttccgg atctgcatcg caggatgctg ctggctaccc tgtggaacac
     4381 ctacatctgt attaacgaag cgctggcatt gaccctgagt gatttttctc tggtcccgcc
     4441 gcatccatac cgccagttgt ttaccctcac aacgttccag taaccgggca tgttcatcat
     4501 cagtaacccg tatcgtgagc atcctctctc gtttcatcgg tatcattacc cccatgaaca
     4561 gaaatccccc ttacacggag gcatcagtga ccaaacagga aaaaaccgcc cttaacatgg
     4621 cccgctttat cagaagccag acattaacgc ttctggagaa actcaacgag ctggacgcgg
     4681 atgaacaggc agacatctgt gaatcgcttc acgaccacgc tgatgagctt taccgcagct
     4741 gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
     4801 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
     4861 gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata
     4921 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga
     4981 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct
     5041 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     5101 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     5161 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     5221 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     5281 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     5341 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     5401 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     5461 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     5521 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     5581 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     5641 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     5701 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     5761 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     5821 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     5881 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     5941 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     6001 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     6061 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     6121 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     6181 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     6241 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg
     6301 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     6361 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     6421 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     6481 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     6541 atagtgtatg cggcgaccga gttgctcttg cccggcgtca acacgggata ataccgcgcc
     6601 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     6661 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     6721 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     6781 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     6841 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     6901 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     6961 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgagg
//
