pQC2123 vector (V003860)

LOCUS       V003860                11256 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003860
VERSION     V003860
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 11256)
  AUTHORS   Chen Q, Lee G, Craig C, Ng V, Carlson PE Jr., Hinton DM, Stibitz S.
  TITLE     A novel Bvg-repressed promoter causes vrg-like transcription of fim3
            but does not result in the production of serotype 3 Fimbriae in the
            Bvg(-) mode Bordetella pertussis
  JOURNAL   J. Bacteriol. (2018) In press
   PUBMED   30061354
REFERENCE   2  (bases 1 to 11256)
  AUTHORS   Chen Q, Lee G, Craig C, Ng V, Carlson P, Hinton D, Stibitz S.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-JUL-2018) Division of Bacterial, Parasitic, and
            Allergenic Products, Center for Biologics Evaluation and Research,
            FDA, 10903 New Hampshire Avenue, Silver Spring, MD 20933, USA
REFERENCE   3  (bases 1 to 11256)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11256)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J.
            Bacteriol. (2018) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (05-JUL-2018) Division of Bacterial, Parasitic, and Allergenic
            Products, Center for Biologics Evaluation and Research, FDA, 10903
            New Hampshire Avenue, Silver Spring, MD 20933, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11256
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(142..750)
                     /gene="lacA"
                     /label="Galactoside O-acetyltransferase"
                     /note="Galactoside O-acetyltransferase from Escherichia
                     coli (strain K12). Accession#: P07464"
     CDS             complement(817..2067)
                     /gene="lacY"
                     /label="Lactose permease"
                     /note="Lactose permease from Escherichia coli (strain K12).
                     Accession#: P02920"
     CDS             complement(2122..5190)
                     /label="lacZ"
                     /note="beta-galactosidase"
     terminator      5532..5575
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      complement(5675..5761)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     promoter        6529..6557
                     /label="Pc promoter"
                     /note="class 1 integron promoter"
     CDS             6746..7276
                     /label="GmR"
                     /note="gentamycin acetyltransferase"
     oriT            complement(7298..7406)
                     /direction=LEFT
                     /label="oriT"
                     /note="incP origin of transfer"
     CDS             8828..9016
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    9121..9261
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(9447..10035)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(10209..11066)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(11067..11171)
                     /label="AmpR promoter"