LOCUS       V003860                11256 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003860
VERSION     V003860
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 11256)
  AUTHORS   Chen Q, Lee G, Craig C, Ng V, Carlson PE Jr., Hinton DM, Stibitz S.
  TITLE     A novel Bvg-repressed promoter causes vrg-like transcription of fim3
            but does not result in the production of serotype 3 Fimbriae in the
            Bvg(-) mode Bordetella pertussis
  JOURNAL   J. Bacteriol. (2018) In press
   PUBMED   30061354
REFERENCE   2  (bases 1 to 11256)
  AUTHORS   Chen Q, Lee G, Craig C, Ng V, Carlson P, Hinton D, Stibitz S.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-JUL-2018) Division of Bacterial, Parasitic, and
            Allergenic Products, Center for Biologics Evaluation and Research,
            FDA, 10903 New Hampshire Avenue, Silver Spring, MD 20933, USA
REFERENCE   3  (bases 1 to 11256)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11256)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J.
            Bacteriol. (2018) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (05-JUL-2018) Division of Bacterial, Parasitic, and Allergenic
            Products, Center for Biologics Evaluation and Research, FDA, 10903
            New Hampshire Avenue, Silver Spring, MD 20933, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11256
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(142..750)
                     /gene="lacA"
                     /label="Galactoside O-acetyltransferase"
                     /note="Galactoside O-acetyltransferase from Escherichia
                     coli (strain K12). Accession#: P07464"
     CDS             complement(817..2067)
                     /gene="lacY"
                     /label="Lactose permease"
                     /note="Lactose permease from Escherichia coli (strain K12).
                     Accession#: P02920"
     CDS             complement(2122..5190)
                     /label="lacZ"
                     /note="beta-galactosidase"
     terminator      5532..5575
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      complement(5675..5761)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     promoter        6529..6557
                     /label="Pc promoter"
                     /note="class 1 integron promoter"
     CDS             6746..7276
                     /label="GmR"
                     /note="gentamycin acetyltransferase"
     oriT            complement(7298..7406)
                     /direction=LEFT
                     /label="oriT"
                     /note="incP origin of transfer"
     CDS             8828..9016
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    9121..9261
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(9447..10035)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(10209..11066)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(11067..11171)
                     /label="AmpR promoter"
ORIGIN
        1 ccctttcgtc ttcaagaatt gcggccgcag gcatgatgcg acgcttgttc ctgcgctttg
       61 ttcatgccgg atgcggctaa tgtagatcgc tgaacttgta ggcctgataa gcgcagcgta
      121 tcaggcaatt tttataattt aaactgacga ttcaacttta taatctttga aataatagtg
      181 cttatcccgg tcgtttattt cgcgaataac ccgacaagga acgccagccg ccacgacgtt
      241 tggtggaatg tcttttgtga cgatactacc cgcgccaata acagaattat ccccgatggt
      301 gacgcctgga ttaataacca catgacttcc gatccagacg ttattgccaa tcgttatcgg
      361 aaaagagtac atctcgccgt tttttctcaa ttcatggtgt acagggtgtc ccgtaacgga
      421 aagagtaacg ttgggtgcaa tcagtacgtt atcaccgatt gttaccgtgt agtcatcgac
      481 aatggttaaa ttgaaatttg cataaaaatt gcggcctata tggatgttgg aaccgtaaga
      541 gaaatagaca ggcggttcta cccaggcgtt ttcccctacc gtggcaaaca tttctttaat
      601 caggctttct cttttttcaa cttctgatgg atgcgagtga ttaaactcat acattaacgt
      661 tttcccacga agtctttttt ccggtaagcc ttcgcacata tcggtaaata gcttgcctgc
      721 tcttattctt tcggtcattg gcatgttcat tgcgatcact ccgttatgat atgttggtcg
      781 gataagcgtc gcgccgcatc cgacattgat tgcttaagcg acttcattca cctgacgacg
      841 cagcagggaa agcgggccgg ggccgctaag cgtgaacacg gaaattaagg tgaagcccag
      901 cgccaccaga cccagcacca gataagcgcc ctggaaaccg atgctttcat acatattgcc
      961 cgccagtaca gacataaaaa tcatcgccag ttgcttaaag aagcagaaac agaccagata
     1021 aatcgtcgct gaaaaacgca cttcaaactg gctggtaata tatttaaagc agcccaccag
     1081 caggaacggt acttcaaaca tatgcagcgt tttcagaata accacttcca gcgctgaggt
     1141 ggcgaacgat gagccaataa tacgtacaga cataatagtg ccagccagca gcagggcgtt
     1201 tttcccaccg atgcgattaa tgatcagtgg cgcaaagaac ataatcgagg cgttaagtaa
     1261 ttcgcccatt gtcgttacgt agccaaatac ccgcgtaccc tgttcaccgg tagcaaagaa
     1321 cgaagtaaag aaattagcaa actgttggtc aaaaacatcg taggtgcagg aaacgccaat
     1381 aacatacagt gacaaaaacc acagttttgg ctgtctgaac agttccagtg ccagcttaag
     1441 gctaaatgcc gaatggttgg cacctaccgc attggcaacc gtggcagaag agggcgcatc
     1501 cgttttggcg aaaaagagta aaacggcgag gatgagtgca cagccagagc ccagccagaa
     1561 aacaaactga ttattgatgg tgaacatgat gccgacaatc gaggcacaca gcgcccagcc
     1621 aacacagcca aacatccgcg cgcgaccaaa ttcgaaatta ctgcgacggc tgactttctc
     1681 aataaatgcc tctactgctg gcgcaccggc gttaaaacaa aagcctagat aaataccacc
     1741 aacaatcgat cctactaaaa tgttgtattg taacagtggc ccgaagataa aaataaagaa
     1801 cggcgcaaac atcactaaca tgccggtaat aatccacagc aggtatttgc gcagcccgag
     1861 tttgtcagaa agcagaccaa acagcggttg gaataatagc gagaacagag aaatagcggc
     1921 aaaaataata cccgtatcac ttttgctgat atggttgatg tcatgtagcc aaatcgggaa
     1981 aaacgggaag taggctccca tgataaaaaa gtaaaagaaa aagaataaac cgaacatcca
     2041 aaagtttgtg ttttttaaat agtacataat ggatttcctt acgcgaaata cgggcagaca
     2101 tggcctgccc ggttattatt atttttgaca ccagaccaac tggtaatggt agcgaccggc
     2161 gctcagctga aattccgccg atactgacgg gctccaggag tcgtcgccac caatccccat
     2221 atggaaaccg tcgatattca gccatgtgcc ttcttccgcg tgcagcagat ggcgatggct
     2281 ggtttccatc agttgctgtt gactgtagcg gctgatgttg aactggaagt cgccgcgcca
     2341 ctggtgtggg ccataattca attcgcgcgt cccgcagcgc agaccgtttt cgctcgggaa
     2401 gacgtacggg gtatacatgt ctgacaatgg cagatcccag cggtcaaaac aggcggcagt
     2461 aaggcggtcg ggatagtttt cttgcggccc taatccgagc cagtttaccc gctctgctac
     2521 ctgcgccagc tggcagttca ggccaatccg cgccggatgc ggtgtatcgc tcgccacttc
     2581 aacatcaacg gtaatcgcca tttgaccact accatcaatc cggtaggttt tccggctgat
     2641 aaataaggtt ttcccctgat gctgccacgc gtgagcggtc gtaatcagca ccgcatcagc
     2701 aagtgtatct gccgtgcact gcaacaacgc tgcttcggcc tggtaatggc ccgccgcctt
     2761 ccagcgttcg acccaggcgt tagggtcaat gcgggtcgct tcacttacgc caatgtcgtt
     2821 atccagcggt gcacgggtga actgatcgcg cagcggcgtc agcagttgtt ttttatcgcc
     2881 aatccacatc tgtgaaagaa agcctgactg gcggttaaat tgccaacgct tattacccag
     2941 ctcgatgcaa aaatccattt cgctggtggt cagatgcggg atggcgtggg acgcggcggg
     3001 gagcgtcaca ctgaggtttt ccgccagacg ccactgctgc caggcgctga tgtgcccggc
     3061 ttctgaccat gcggtcgcgt tcggttgcac tacgcgtact gtgagccaga gttgcccggc
     3121 gctctccggc tgcggtagtt caggcagttc aatcaactgt ttaccttgtg gagcgacatc
     3181 cagaggcact tcaccgcttg ccagcggctt accatccagc gccaccatcc agtgcaggag
     3241 ctcgttatcg ctatgacgga acaggtattc gctggtcact tcgatggttt gcccggataa
     3301 acggaactgg aaaaactgct gctggtgttt tgcttccgtc agcgctggat gcggcgtgcg
     3361 gtcggcaaag accagaccgt tcatacagaa ctggcgatcg ttcggcgtat cgccaaaatc
     3421 accgccgtaa gccgaccacg ggttgccgtt ttcatcatat ttaatcagcg actgatccac
     3481 ccagtcccag acgaagccgc cctgtaaacg gggatactga cgaaacgcct gccagtattt
     3541 agcgaaaccg ccaagactgt tacccatcgc gtgggcgtat tcgcaaagga tcagcgggcg
     3601 cgtctctcca ggtagcgaaa gccatttttt gatggaccat ttcggcacag ccgggaaggg
     3661 ctggtcttca tccacgcgcg cgtacatcgg gcaaataata tcggtggccg tggtgtcggc
     3721 tccgccgcct tcatactgca ccgggcggga aggatcgaca gatttgatcc agcgatacag
     3781 cgcgtcgtga ttagcgccgt ggcctgattc attccccagc gaccagatga tcacactcgg
     3841 gtgattacga tcgcgctgca ccattcgcgt tacgcgttcg ctcatcgccg gtagccagcg
     3901 cggatcatcg gtcagacgat tcattggcac catgccgtgg gtttcaatat tggcttcatc
     3961 caccacatac aggccgtagc ggtcgcacag cgtgtaccac agcggatggt tcggataatg
     4021 cgaacagcgc acggcgttaa agttgttctg cttcatcagc aggatatcct gcaccatcgt
     4081 ctgctcatcc atgacctgac catgcagagg atgatgctcg tgacggttaa cgcctcgaat
     4141 cagcaacggc ttgccgttca gcagcagcag accattttca atccgcacct cgcggaaacc
     4201 gacatcgcag gcttctgctt caatcagcgt gccgtcggcg gtgtgcagtt caaccaccgc
     4261 acgatagaga ttcgggattt cggcgctcca cagtttcggg ttttcgacgt tcagacgtag
     4321 tgtgacgcga tcggcataac caccacgctc atcgataatt tcaccgccga aaggcgcggt
     4381 gccgctggcg acctgcgttt caccctgcca taaagaaact gttacccgta ggtagtcacg
     4441 caactcgccg cacatctgaa cttcagcctc cagtacagcg cggctgaaat catcattaaa
     4501 gcgagtggca acatggaaat cgctgatttg tgtagtcggt ttatgcagca acgagacgtc
     4561 acggaaaatg ccgctcatcc gccacatatc ctgatcttcc agataactgc cgtcactcca
     4621 acgcagcacc atcaccgcga ggcggttttc tccggcgcgt aaaaatgcgc tcaggtcaaa
     4681 ttcagacggc aaacgactgt cctggccgta accgacccag cgcccgttgc accacagatg
     4741 aaacgccgag ttaacgccat caaaaataat tcgcgtctgg ccttcctgta gccagctttc
     4801 atcaacatta aatgtgagcg agtaacaacc cgtcggattc tccgtgggaa caaacggcgg
     4861 attgaccgta atgggatagg ttacgttggt gtagatgggc gcatcgtaac cgtgcatctg
     4921 ccagtttgag gggacgacga cagtatcggc ctcaggaaga tcgcactcca gccagctttc
     4981 cggcaccgct tctggtgccg gaaaccaggc aaagcgccat tcgccattca ggctgcgcaa
     5041 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg
     5101 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa
     5161 aacgacggcc agtgaatccg taatcatggt gtcgacggat ccgaattctt aattaacaat
     5221 tctctagaca attccaattc gtaattggag tagaactgaa atcgtcttgt gcatttaatg
     5281 cgattggcgc caagttttta gaaggataat aattattagg tgcaagtatt tgacttaatt
     5341 ggttttcctt agctttaatt gtcgcgagtc aattcttaat taacaattct ctagacaatt
     5401 ccgatcccca attcctggca gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt
     5461 gcttcgcaac gttcaaatcc gctcccggcg gatttgtcct actcaggaga gcgttcaccg
     5521 acaaacaaca gataaaacga aaggcccagt ctttcgactg agcctttcgt tttatttgat
     5581 gcctggggaa ttaattcctg gcagtttatg gcgggcgtcc tgcccgccac cctccgggcc
     5641 gttgcttcgc aacgttcaaa tccgctcccg gcggatttgt cctactcagg agagcgttca
     5701 ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt
     5761 gatgcctggg gaattgggaa ttgatcgctg taatccgggc agcgcaacgg aacattcatc
     5821 agtgtaaaaa tggaatcaat aaagccctgc gcagcgcgca gggtcagcct gaatacgcgt
     5881 ttaatgacca gcacagtcgt gatggcaagg tcagaatagc gctgaggtct gcctcgtgaa
     5941 gaaggtgttg ctgactcata ccaggcctga atcgccccat catccagcca gaaagtgagg
     6001 gagccacggt tgatgagagc tttgttgtag gtggaccagt tggtgatttt gaacttttgc
     6061 tttgccacgg aacggtctgc gttgtcggga agatgcgtga tctgatcctt caactcagca
     6121 aaagttcgat ttattcaaca aagccgccgt cccgtcaagt cagcgtaatg ctctgccagt
     6181 gttacaacca attaaccaat tctgattaga aaaactcatc gagcatcaaa tgaaactgca
     6241 atttattcat atcaggatta tcaataccat atttttgaaa aagccgtttc tgtaatgaag
     6301 gagaaaactc accgaggcag ttccatagga tggcaagatc ctggtatcgg tctgcgattc
     6361 cgactcgtcc aacatcaata caacctatta atttcccctc gtcaaaaata aggttatcaa
     6421 gtgagaaatc accatgagtg acgactgaat ccggtgagaa tggcaaaagc tgcggccgtt
     6481 taaacggccg caattccacc gtggaaacgg atgaaggcac gaacccagtt gacataagcc
     6541 tgttcggttc gtaaactgta atgcaagtag cgtatgcgct cacgcaactg gtccagaacc
     6601 ttgaccgaac gcagcggtgg taacggcgca gtggcggttt tcatggcttg ttatgactgt
     6661 ttttttgtac agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga
     6721 tgtttgatgt tatggagcag caacgatgtt acgcagcagc aacgatgtta cgcagcaggg
     6781 cagtcgccct aaaacaaagt taggtggctc aagtatgggc atcattcgca catgtaggct
     6841 cggccctgac caagtcaaat ccatgcgggc tgctcttgat cttttcggtc gtgagttcgg
     6901 agacgtagcc acctactccc aacatcagcc ggactccgat tacctcggga acttgctccg
     6961 tagtaagaca ttcatcgcgc ttgctgcctt cgaccaagaa gcggttgttg gcgctctcgc
     7021 ggcttacgtt ctgcccaggt ttgagcagcc gcgtagtgag atctatatct atgatctcgc
     7081 agtctccggc gagcaccgga ggcagggcat tgccaccgcg ctcatcaatc tcctcaagca
     7141 tgaggccaac gcgcttggtg cttatgtgat ctacgtgcaa gcagattacg gtgacgatcc
     7201 cgcagtggct ctctatacaa agttgggcat acgggaagaa gtgatgcact ttgatatcga
     7261 cccaagtacc gccacctaaa agctgtttaa acagcttcgg ccagcctcgc agagcaggat
     7321 tcccgttgag caccgccagg tgcgaataag ggacagtgaa gaaggaacac ccgctcgcgg
     7381 gtgggcctac ttcacctatc ctgcccggct gacgccgttg gatacaccaa ggaaagtcta
     7441 cacgaaccct ttggcaaaat cctgtatatc gtgcgaaaaa ggatggatat accgaaaaaa
     7501 tcgctataat gaccccgaag cagggttatg cagcggaaaa gcgcgatccg tcgagattta
     7561 aatctcgacc gatgcccttg agagccttca acccagtcag ctccttccgg tgggcgcggg
     7621 gcatgactat cgtcgccgca cttatgactg tcttctttat catgcaactc gtaggacagg
     7681 tgccggcagc gctctgggtc attttcggcg aggaccgctt tcgctggagc gcgacgatga
     7741 tcggcctgtc gcttgcggta ttcggaatct tgcacgccct cgctcaagcc ttcgtcactg
     7801 gtcccgccac caaacgtttc ggcgagaagc aggccattat cgccggcatg gcggccgacg
     7861 cgctgggcta cgtcttgctg gcgttcgcga cgcgaggctg gatggccttc cccattatga
     7921 ttcttctcgc ttccggcggc atcgggatgc ccgcgttgca ggccatgctg tccaggcagg
     7981 tagatgacga ccatcaggga cagcttcaag gatcgctcgc ggctcttacc agcctaactt
     8041 cgatcactgg accgctgatc gtcacggcga tttatgccgc ctcggcgagc acatggaacg
     8101 ggttggcatg gattgtaggc gccgccctat accttgtctg cctccccgcg ttgcgtcgcg
     8161 gtgcatggag ccgggccacc tcgacctgaa tggaagccgg cggcacctcg ctaacggatt
     8221 caccactcca agaattggag ccaatcaatt cttgcggaga actgtgaatg cgcaaaccaa
     8281 cccttggcag aacatatcca tcgcgtccgc catctccagc agccgcacgc ggcgcatctc
     8341 gggcagcgtt gggtcctggc cacgggtgcg catgatcgtg ctcctgtcgt tgaggacccg
     8401 gctaggctgg cggggttgcc ttactggtta gcagaatgaa tcaccgatac gcgagcgaac
     8461 gtgaagcgac tgctgctgca aaacgtctgc gacctgagca acaacatgaa tggtcttcgg
     8521 tttccgtgtt tcgtaaagtc tggaaacgcg gaagtcagcg ccctgcacca ttatgttccg
     8581 gatctgcatc gcaggatgct gctggctacc ctgtggaaca cctacatctg tattaacgaa
     8641 gcgctggcat tgaccctgag tgatttttct ctggtcccgc cgcatccata ccgccagttg
     8701 tttaccctca caacgttcca gtaaccgggc atgttcatca tcagtaaccc gtatcgtgag
     8761 catcctctct cgtttcatcg gtatcattac ccccatgaac agaaatcccc cttacacgga
     8821 ggcatcagtg accaaacagg aaaaaaccgc ccttaacatg gcccgcttta tcagaagcca
     8881 gacattaacg cttctggaga aactcaacga gctggacgcg gatgaacagg cagacatctg
     8941 tgaatcgctt cacgaccacg ctgatgagct ttaccgcagc tgcctcgcgc gtttcggtga
     9001 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc
     9061 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
     9121 cgcagccatg acccagtcac gtagcgatag cggagtgtat actggcttaa ctatgcggca
     9181 tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta
     9241 aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     9301 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     9361 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     9421 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     9481 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     9541 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     9601 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     9661 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     9721 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     9781 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     9841 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     9901 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     9961 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
    10021 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
    10081 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
    10141 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
    10201 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
    10261 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
    10321 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
    10381 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
    10441 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
    10501 cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct
    10561 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
    10621 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
    10681 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
    10741 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
    10801 agttgctctt gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa
    10861 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
    10921 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
    10981 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
    11041 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
    11101 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
    11161 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
    11221 atgacattaa cctataaaaa taggcgtatc acgagg
//
