pPyCAG_BstXI_IB vector (V003885)

LOCUS       40924_35728        6741 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pPyCAG_BstXI_IB, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6741)
  AUTHORS   Hemmrich G, Khalturin K, Boehm AM, Puchert M, Anton-Erxleben F, 
            Wittlieb J, Klostermeier UC, Rosenstiel P, Oberg HH, Domazet-Loso T,
            Sugimoto T, Niwa H, Bosch TC.
  TITLE     Molecular signatures of the three stem cell lineages in hydra and 
            the emergence of stem cell function at the base of multicellularity
  JOURNAL   Mol. Biol. Evol. 29 (11), 3267-3280 (2012)
  PUBMED    22595987
REFERENCE   2  (bases 1 to 6741)
  AUTHORS   Hemmrich G, Khalturin K, Boehm A-M., Puchert M, Anton-Erxleben F, 
            Wittlieb J, Klostermeier UC, Rosenstiel P, Oberg H-H., Domazet-Loso 
            T, Sugimoto T, Niwa H, Bosch TCG.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2012) Zoological Institute, Christian-Albrechts 
            University, Olshausenstrasse 40, Kiel 24098, Germany
REFERENCE   3  (bases 1 to 6741)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6741)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol. Biol. 
            Evol."; date: "2012"; volume: "29"; issue: "11"; pages: "3267-3280"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (27-APR-2012) Zoological Institute, Christian-Albrechts University, 
            Olshausenstrasse 40, Kiel 24098, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6741
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    576..710
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        724..1103
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1105..1382
                     /label=chicken beta-actin promoter
     intron          1383..2399
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
     CDS             2454..3617
                     /codon_start=1
                     /product="transcription factor HySox1/2/3"
                     /label=transcription factor HySox1/2/3
                     /protein_id="AFK74894.1"
                     /translation="MATAINVIQHPSDANNDTHFKQVVGYNTSQSVLVPQNLNLSNRVS
                     NSLVSDHETSKQEDFDKVKRPMNSFMVWSREKRRRLAHENPKMHNSEISKRLGAEWKVL
                     TEDEKAPFVFEAKRLRAEHMKSHPDYKYRPRRKAKTSSKKNEQKMPHVITTDGKQIFMP
                     TQYTSGYAITGGINYPFNGYLSELVNGHEAYASNTSPSAVYGFPHGATIAVTSTISQSQ
                     LPSSSSSSVTAATGLSAQYSVVHSGTPTYMYSPLGFPYLNGAAMGTYGGQPYSHALTLK
                     PDTSSITVSTEKATSHLSMHHDAVRSTIAPSVYYPIGIYPTAVDHNCNLTYDPKIPYAT
                     MTLLEQPHGNVKREQTREGTTRNISDDHQSRRVNTPTYSISSNTATQ"
     misc_feature    3633..4219
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             4223..4609
                     /codon_start=1
                     /label=BSD
                     /note="blasticidin S deaminase"
                     /translation="PLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGVNV
                     YHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIKAI
                     VKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    4732..4866
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(5105..5693)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5867..6724)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"