LOCUS       40924_35728        6741 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pPyCAG_BstXI_IB, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6741)
  AUTHORS   Hemmrich G, Khalturin K, Boehm AM, Puchert M, Anton-Erxleben F, 
            Wittlieb J, Klostermeier UC, Rosenstiel P, Oberg HH, Domazet-Loso T,
            Sugimoto T, Niwa H, Bosch TC.
  TITLE     Molecular signatures of the three stem cell lineages in hydra and 
            the emergence of stem cell function at the base of multicellularity
  JOURNAL   Mol. Biol. Evol. 29 (11), 3267-3280 (2012)
  PUBMED    22595987
REFERENCE   2  (bases 1 to 6741)
  AUTHORS   Hemmrich G, Khalturin K, Boehm A-M., Puchert M, Anton-Erxleben F, 
            Wittlieb J, Klostermeier UC, Rosenstiel P, Oberg H-H., Domazet-Loso 
            T, Sugimoto T, Niwa H, Bosch TCG.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2012) Zoological Institute, Christian-Albrechts 
            University, Olshausenstrasse 40, Kiel 24098, Germany
REFERENCE   3  (bases 1 to 6741)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6741)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol. Biol. 
            Evol."; date: "2012"; volume: "29"; issue: "11"; pages: "3267-3280"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (27-APR-2012) Zoological Institute, Christian-Albrechts University, 
            Olshausenstrasse 40, Kiel 24098, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6741
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    576..710
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        724..1103
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1105..1382
                     /label=chicken beta-actin promoter
     intron          1383..2399
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
     CDS             2454..3617
                     /codon_start=1
                     /product="transcription factor HySox1/2/3"
                     /label=transcription factor HySox1/2/3
                     /protein_id="AFK74894.1"
                     /translation="MATAINVIQHPSDANNDTHFKQVVGYNTSQSVLVPQNLNLSNRVS
                     NSLVSDHETSKQEDFDKVKRPMNSFMVWSREKRRRLAHENPKMHNSEISKRLGAEWKVL
                     TEDEKAPFVFEAKRLRAEHMKSHPDYKYRPRRKAKTSSKKNEQKMPHVITTDGKQIFMP
                     TQYTSGYAITGGINYPFNGYLSELVNGHEAYASNTSPSAVYGFPHGATIAVTSTISQSQ
                     LPSSSSSSVTAATGLSAQYSVVHSGTPTYMYSPLGFPYLNGAAMGTYGGQPYSHALTLK
                     PDTSSITVSTEKATSHLSMHHDAVRSTIAPSVYYPIGIYPTAVDHNCNLTYDPKIPYAT
                     MTLLEQPHGNVKREQTREGTTRNISDDHQSRRVNTPTYSISSNTATQ"
     misc_feature    3633..4219
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             4223..4609
                     /codon_start=1
                     /label=BSD
                     /note="blasticidin S deaminase"
                     /translation="PLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGVNV
                     YHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIKAI
                     VKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    4732..4866
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(5105..5693)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5867..6724)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 gaccgctttg gaaaaacaaa gactgtattt cctggaaatt aatgtttatt caataaactg
       61 tgtattcagc tatattcaca tagtggtgag gctgaaatga ggcgggaaga ggcggttggg
      121 gcttaattat atcaatttgg gtggccccac agcgcctcca aggcgccagt cctgttttga
      181 caagttgcct ctggaagcct ctcttctttt tctccagagt aagcggaggc caggggcccc
      241 cggcctctgc ttaatactaa aaaaaacagc tgttgtcata gtaatgattg ggtggaaaca
      301 ttccaggcct gggtggagag gctttttgct tcctcttgca aaaccacact gacattccag
      361 gcctgggtgg agaggctttt tgcttcctct tgcaaaacca cactgccctc tggagggcag
      421 ttgcctagca actaattaaa agaggatgtc gcacggccag ctgcggtcag ttagtcactt
      481 cctgcttaac tgacttgaca ttttctattt taagagtcgg gaggaaaatt actgtgttgg
      541 aggccctccg ccatcttctg aagctgaatc gaattaactt gtttattgca gcttataatg
      601 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
      661 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc tgatatcatc
      721 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
      781 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      841 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      901 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      961 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
     1021 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
     1081 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca
     1141 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag
     1201 cgatgggggc gggggggggg ggggggcgcg cgccaggcgg ggcggggcgg ggcgaggggc
     1261 ggggcggggc gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt
     1321 ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg
     1381 cgggagtcgc tgcgcgctgc cttcgccccg tgccccgctc cgccgccgcc tcgcgccgcc
     1441 cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc
     1501 ctccgggctg taattagcgc ttggtttaat gacggcttgt ttcttttctg tggctgcgtg
     1561 aaagccttga ggggctccgg gagggccctt tgtgcggggg gagcggctcg gggggtgcgt
     1621 gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc gctgcccggc ggctgtgagc
     1681 gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt gcgcgagggg agcgcggccg
     1741 ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa caaaggctgc gtgcggggtg
     1801 tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt cgggctgcaa ccccccctgc
     1861 acccccctcc ccgagttgct gagcacggcc cggcttcggg tgcggggctc cgtacggggc
     1921 gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg
     1981 cggggccgcc tcgggccggg gagggctcgg gggaggggcg cggcggcccc cggagcgccg
     2041 gcggctgtcg aggcgcggcg agccgcagcc attgcctttt atggtaatcg tgcgagaggg
     2101 cgcagggact tcctttgtcc caaatctgtg cggagccgaa atctgggagg cgccgccgca
     2161 ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa atgggcgggg
     2221 agggccttcg tgcgtcgccg cgccgccgtc cccttctccc tctccagcct cggggctgtc
     2281 cgcgggggga cggctgcctt cgggggggac ggggcagggc ggggttcggc ttctggcgtg
     2341 tgaccggcgg ctctagagcc tctgctaacc atgttcatgc cttcttcttt ttcctacagc
     2401 tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa ttcatggcca
     2461 ctgcaattaa tgttattcaa catccctctg acgccaataa tgatactcat tttaagcaag
     2521 ttgtaggtta caacacttcg caatccgtgc ttgttccaca aaatttaaac ctatcaaatc
     2581 gtgtatctaa ttctcttgtt tccgaccatg aaacttctaa acaagaagat tttgataagg
     2641 tcaaaagacc gatgaactct ttcatggtgt ggtctcgaga gaagcgacga agactcgcac
     2701 atgaaaatcc taaaatgcat aacagtgaaa taagtaaacg cttgggagcc gagtggaaag
     2761 ttttaactga agatgaaaaa gctccttttg tttttgaagc aaagcgttta agagctgaac
     2821 atatgaaatc acatcctgat tacaagtata gaccacgtcg taaagctaag acttcatcaa
     2881 aaaaaaacga acagaagatg cctcatgtaa taacaactga tgggaaacaa attttcatgc
     2941 ccacacagta cacttctgga tatgcaataa caggaggtat taattacccc tttaatggtt
     3001 atttatctga actagtaaat ggacatgaag catatgcaag taatacatca ccatcagctg
     3061 tctatggctt tccacatgga gcaaccattg cagtgaccag tactatatca caatcacaat
     3121 tgccttcttc atcaagttcc tcagtaacag ctgcaactgg tttatctgcg caatacagtg
     3181 ttgtacattc aggtactcca acttacatgt atagtccgtt aggatttcca tatcttaatg
     3241 gtgctgctat gggtacatat ggtgggcagc cttattcaca tgctctaaca cttaaaccag
     3301 acacatcgtc tatcactgta tcaactgaaa aagcaactag tcatttatca atgcatcatg
     3361 atgctgttcg ttcaacaatt gcaccaagtg tttattaccc gattggtatt tatccgactg
     3421 ctgttgatca caattgtaac ctaacgtatg atccaaaaat accatatgca acaatgactt
     3481 tattagaaca acctcatgga aatgtgaaac gtgaacaaac tcgtgagggt acaacacgga
     3541 atatttcaga tgatcaccaa agtagaagag ttaatacacc tacatattct atttcgtcca
     3601 acactgcaac gcagtaggaa ttccgccccc cccccctctc cctccccccc ccctaacgtt
     3661 actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc
     3721 atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc
     3781 attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag
     3841 gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg
     3901 cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat
     3961 acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga
     4021 gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc
     4081 cattgtatgg gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg
     4141 ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga
     4201 tgataatatg gccacaacca tgcctttgtc tcaagaagaa tccaccctca ttgaaagagc
     4261 aacggctaca atcaacagca tccccatctc tgaagactac agcgtcgcca gcgcagctct
     4321 ctctagcgac ggccgcatct tcactggtgt caatgtatat cattttactg ggggaccttg
     4381 tgcagaactc gtggtgctgg gcactgctgc tgctgcggca gctggcaacc tgacttgtat
     4441 cgtcgcgatc ggaaatgaga acaggggcat cttgagcccc tgcggacggt gccgacaggt
     4501 gcttctcgat ctgcatcctg ggatcaaagc catagtgaag gacagtgatg gacagccgac
     4561 ggcagttggg attcgtgaat tgctgccctc tggttatgtg tgggagggtt aactagataa
     4621 gtaatgatca taatcagcca tatcacatct gtagaggttt tacttgcttt aaaaaacctc
     4681 ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt
     4741 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     4801 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     4861 tggatccgct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     4921 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     4981 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     5041 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     5101 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     5161 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     5221 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     5281 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     5341 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     5401 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     5461 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     5521 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     5581 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     5641 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     5701 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     5761 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     5821 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     5881 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     5941 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     6001 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     6061 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     6121 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     6181 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     6241 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     6301 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     6361 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     6421 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     6481 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     6541 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     6601 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     6661 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     6721 tcatactctt cctttttcaa t
//
