Basic Vector Information
- Vector Name:
- pPLV02_v2
- Antibiotic Resistance:
- Kanamycin
- Length:
- 4555 bp
- Type:
- Cloning vector
- Replication origin:
- pSa ori
- Source/Author:
- Wendrich JR, Liao CY, van den Berg WA, De Rybel B, Weijers D.
pPLV02_v2 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pPLV02_v2 vector Sequence
LOCUS 40924_35079 4555 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector pPLV02_v2, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4555)
AUTHORS Wendrich JR, Liao CY, van den Berg WA, De Rybel B, Weijers D.
TITLE Ligation-independent cloning for plant research
JOURNAL Methods Mol. Biol. 1284, 421-431 (2015)
PUBMED 25757785
REFERENCE 2 (bases 1 to 4555)
AUTHORS Wendrich JR, Liao C-Y., van den Berg WAM., De Rybel B, Weijers D.
TITLE Direct Submission
JOURNAL Submitted (25-JUN-2014) Laboratory of Biochemistry, Wageningen
University, Dreijenlaan 3, Wageningen 6703HA, The Netherlands
REFERENCE 3 (bases 1 to 4555)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 4555)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Methods
Mol. Biol. 1284, 421-431 (2015)"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(25-JUN-2014) Laboratory of Biochemistry, Wageningen University,
Dreijenlaan 3, Wageningen 6703HA, The Netherlands"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..4555
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 165..181
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 188..206
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind 232..248
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
misc_feature 269..298
/note="LIC; ligation-independent cloning (LIC) site"
terminator 312..560
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
misc_feature 582..606
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
rep_origin complement(697..1285)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(1459..2271)
/label=KanR
/note="aminoglycoside phosphotransferase"
rep_origin 2562..2997
/label=pSa ori
/note="origin of replication from bacterial plasmid pSa"
misc_feature 3124..3146
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA
(truncated)"
terminator complement(3170..3422)
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
CDS complement(3465..4256)
/label=NeoR/KanR
/note="aminoglycoside phosphotransferase"
promoter complement(4290..4469)
/label=NOS promoter
/note="nopaline synthase promoter"
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