LOCUS       40924_35079        4555 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pPLV02_v2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4555)
  AUTHORS   Wendrich JR, Liao CY, van den Berg WA, De Rybel B, Weijers D.
  TITLE     Ligation-independent cloning for plant research
  JOURNAL   Methods Mol. Biol. 1284, 421-431 (2015)
  PUBMED    25757785
REFERENCE   2  (bases 1 to 4555)
  AUTHORS   Wendrich JR, Liao C-Y., van den Berg WAM., De Rybel B, Weijers D.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2014) Laboratory of Biochemistry, Wageningen 
            University, Dreijenlaan 3, Wageningen 6703HA, The Netherlands
REFERENCE   3  (bases 1 to 4555)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4555)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Mol. Biol. 1284, 421-431 (2015)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (25-JUN-2014) Laboratory of Biochemistry, Wageningen University, 
            Dreijenlaan 3, Wageningen 6703HA, The Netherlands"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4555
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     165..181
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        188..206
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     232..248
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    269..298
                     /note="LIC; ligation-independent cloning (LIC) site"
     terminator      312..560
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    582..606
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(697..1285)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1459..2271)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      2562..2997
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     misc_feature    3124..3146
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA
                     (truncated)"
     terminator      complement(3170..3422)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(3465..4256)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(4290..4469)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
ORIGIN
        1 aacattaacg tttacaattt cgcgccattc gccattcagg ctgcgcaact gttgggaagg
       61 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag
      121 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag
      181 tgaattgtaa tacgactcac tatagggcga attgggtacc gggccccccc tcgaggtcga
      241 cggtatcgat aagcttgata tcgaattcta gttggaatgg gttaacccaa ctccataagg
      301 atccgatccc cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg
      361 ccggtcttgc gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta
      421 acatgtaatg catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat
      481 acatttaata cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg
      541 cggtgtcatc tatgtctaga gcggccgcca cgcgtaggcc ttgacaggat atattggcgg
      601 gtaaactaag tcgctgtatg tgtttgtttg agatctcatg tgagcaaaag gccagcaaaa
      661 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
      721 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
      781 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
      841 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
      901 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
      961 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     1021 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     1081 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     1141 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaagaagag ttggtagctc
     1201 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     1261 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     1321 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     1381 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgtgta
     1441 acattggtct agtgattaga aaaactcatc gagcatcaaa tgaaactgca atttattcat
     1501 atcaggatta tcaataccat atttttgaaa aagccgtttc tgtaatgaag gagaaaactc
     1561 accgaggcag ttccatagga tggcaagatc ctggtatcgg tctgcgattc cgactcgtcc
     1621 aacatcaata caacctatta atttcccctc gtcaaaaata aggttatcaa gtgagaaatc
     1681 accatgagtg acgactgaat ccggtgagaa tggcaaaagt ttatgcattt ctttccagac
     1741 ttgttcaaca ggccagccat tacgctcgtc atcaaaatca ctcgcatcaa ccaaaccgtt
     1801 attcattcgt gattgcgcct gagcgagacg aaatacgcga tcgctgttaa aaggacaatt
     1861 acaaacagga atcgaatgca accggcgcag gaacactgcc agcgcatcaa caatattttc
     1921 acctgaatca ggatattctt ctaatacctg gaatgctgtt ttccctggga tcgcagtggt
     1981 gagtaaccat gcatcatcag gagtacggat aaaatgcttg atggtcggaa gaggcataaa
     2041 ttccgtcagc cagtttagtc tgaccatctc atctgtaaca acattggcaa cgctaccttt
     2101 gccatgtttc agaaacaact ctggcgcatc gggcttccca tacaatcggt agattgtcgc
     2161 acctgattgc ccgacattat cgcgagccca tttataccca tataaatcag catccatgtt
     2221 ggaatttaat cgcggccttg agcaagacgt ttcccgttga atatggctca taacacccct
     2281 tgtattactg tttatgtaag cagacagttt tattgttcat gatgatatat ttttatcttg
     2341 tgcaatgtaa catcagagat tttgagacac aacgtggctt tgttgaataa atcgaacttt
     2401 tgctgagttg aaggatcaga tcacgcatct tcccgacaac gcagaccgtt ccgtggcaaa
     2461 gcaaaagttc aaaatcacca actggtccac ctacaacaaa gctctcatca accgtggctc
     2521 cctcactttc tggctggatg atggggcgat tcaggcgatc cccatccaac agcccgccgt
     2581 cgagcgggct tttttatccc cggaagcctg tggatagagg gtagttatcc acgtgaaacc
     2641 gctaatgccc cgcaaagcct tgattcacgg ggctttccgg cccgctccaa aaactatcca
     2701 cgtgaaatcg ctaatcaggg tacgtgaaat cgctaatcgg agtacgtgaa atcgctaata
     2761 aggtcacgtg aaatcgctaa tcaaaaaggc acgtgagaac gctaatagcc ctttcagatc
     2821 aacagcttgc aaacacccct cgctccggca agtagttaca gcaagtagta tgttcaatta
     2881 gcttttcaat tatgaatata tatatcaatt attggtcgcc cttggcttgt ggacaatgcg
     2941 ctacgcgcac cggctccgcc cgtggacaac cgcaagcggt tgcccaccgt cgagcgcctt
     3001 tgcccacaac ccggcggccg gccgcaacag atcgttttat aaattttttt ttttgaaaaa
     3061 gaaaaagccc gaaaggcggc aacctctcgg gcttctggat ttccgatccc cggaattaga
     3121 tcttggcagg atatattgtg gtgtaacgtt atcagcttgc atgccggtcg atctagtaac
     3181 atagatgaca ccgcgcgcga taatttatcc tagtttgcgc gctatatttt gttttctatc
     3241 gcgtattaaa tgtataattg cgggactcta atcataaaaa cccatctcat aaataacgtc
     3301 atgcattaca tgttaattat tacatgctta acgtaattca acagaaatta tatgataatc
     3361 atcgcaagac cggcaacagg attcaatctt aagaaacttt attgccaaat gtttgaacga
     3421 tctgcttgac tctagctaga gtccgaaccc cagagtcccg ctcagaagaa ctcgtcaaga
     3481 aggcgataga aggcgatgcg ctgcgaatcg ggagcggcga taccgtaaag cacgaggaag
     3541 cggtcagccc attcgccgcc aagctcttca gcaatatcac gggtagccaa cgctatgtcc
     3601 tgatagcggt ccgccacacc cagccggcca cagtcgatga atccagaaaa gcggccattt
     3661 tccaccatga tattcggcaa gcaggcatcg ccgtgggtca cgacgagatc ctcgccgtcg
     3721 ggcatccgcg ccttgagcct ggcgaacagt tcggctggcg cgagcccctg atgctcttcg
     3781 tccagatcat cctgatcgac aagaccggct tccatccgag tacgtgctcg ctcgatgcga
     3841 tgtttcgctt ggtggtcgaa tgggcaggta gccggatcaa gcgtatgcag ccgccgcatt
     3901 gcatcagcca tgatggatac tttctcggca ggagcaaggt gagatgacag gagatcctgc
     3961 cccggcactt cgcccaatag cagccagtcc cttcccgctt cagtgacaac gtcgagcaca
     4021 gctgcgcaag gaacgcccgt cgtggccagc cacgatagcc gcgctgcctc gtcctgcagt
     4081 tcattcaggg caccggacag gtcggtcttg acaaaaagaa ccgggcgccc ctgcgctgac
     4141 agccggaaca cggcggcatc agagcagccg attgtctgtt gtgcccagtc atagccgaat
     4201 agcctctcca cccaagcggc cggagaacct gcgtgcaatc catcttgttc aatcatgcct
     4261 cgatcgagtt gagagtgaat atgagactct aattggatac cgaggggaat ttatggaacg
     4321 tcagtggagc atttttgaca agaaatattt gctagctgat agtgacctta ggcgactttt
     4381 gaacgcgcaa taatggtttc tgacgtatgt gcttagctca ttaaactcca gaaacccgcg
     4441 gctcagtggc tccttcaacg ttgcggttct gtcagttcca aacgtaaaac ggcttgtccc
     4501 gcgtcatcgg cgggggtcat aacgtgactc ccttaattct ccgctcatga tcgat
//
