pPLuc vector (V004010)

LOCUS       40924_35059        5917 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Synthetic construct cloning vector pPLuc, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5917)
  AUTHORS   Chen A, Kao YF, Brown CM.
  TITLE     Translation of the first upstream ORF in the hepatitis B virus 
            pregenomic RNA modulates translation at the core and polymerase 
            initiation codons
  JOURNAL   Nucleic Acids Res. 33 (4), 1169-1181 (2005)
  PUBMED    15731337
REFERENCE   2  (bases 1 to 5917)
  AUTHORS   Chen AB, Kao AY-F., Brown CM.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2000) Chris M. Brown, University of Otago, 
            Department of Biochemistry; P.O.Box 56, Dunedin, Otago 9001, New 
            Zealand (E-mail:chris.brown@stonebow.otago.ac.nz, 
            Tel:+64-3-479-7875, Fax:+64-3-479-7866)
REFERENCE   3  (bases 1 to 5917)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5917)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2005"; volume: "33"; issue: "4"; pages: 
            "1169-1181"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (27-JAN-2000) Chris M. Brown, University of Otago, Department of 
            Biochemistry"; volume: " P.O.Box 56, Dunedin, Otago 9001, New 
            Zealand (E-mail:chris.brown@stonebow.otago.ac.nz, 
            Tel:+64-3-479-7875, Fax"; pages: "+64-3-479-7866"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5917
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..495
                     /label=HBV Adw 5' leader of pregenomic RNA
                     /note="HBV Adw 5' leader of pregenomic RNA"
     CDS             35..94
                     /codon_start=1
                     /gene="C0"
                     /label=C0
                     /note="unnamed protein product; ORF"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /protein_id="BAA93573.1"
                     /translation="MSHCSSLQAVPWVALGHGH"
     gene            35..94
                     /gene="C0"
                     /label=C0
     CDS             84..581
                     /codon_start=1
                     /product="modified C"
                     /label=modified C
                     /protein_id="BAA93574.1"
                     /translation="MDIDPYKEFGATVELLSFLPSDFFPSVRDLLDTASALYREALESP
                     EHCSPHHTALRQAILCWGELMTLATWVGNNLEDPATRDLVVNYVNTNMGLKIRQLLWFH
                     ISCLTFGRETVLEYLVSFGVWIRTPPAYRPPNARRRRRQKHKERPGAILSAGRWNRWRA
                     TA"
     CDS             499..2148
                     /codon_start=1
                     /label=luciferase
                     /note="firefly luciferase"
                     /translation="VEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    complement(2361..2482)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3147..3735)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3909..4766)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4767..4871)
                     /label=AmpR promoter
     rep_origin      4898..5353
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    5484..5532
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    5546..5637
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
     promoter        5696..5892
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        5899..5917
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"