LOCUS       40924_35059        5917 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Synthetic construct cloning vector pPLuc, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5917)
  AUTHORS   Chen A, Kao YF, Brown CM.
  TITLE     Translation of the first upstream ORF in the hepatitis B virus 
            pregenomic RNA modulates translation at the core and polymerase 
            initiation codons
  JOURNAL   Nucleic Acids Res. 33 (4), 1169-1181 (2005)
  PUBMED    15731337
REFERENCE   2  (bases 1 to 5917)
  AUTHORS   Chen AB, Kao AY-F., Brown CM.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2000) Chris M. Brown, University of Otago, 
            Department of Biochemistry; P.O.Box 56, Dunedin, Otago 9001, New 
            Zealand (E-mail:chris.brown@stonebow.otago.ac.nz, 
            Tel:+64-3-479-7875, Fax:+64-3-479-7866)
REFERENCE   3  (bases 1 to 5917)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5917)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2005"; volume: "33"; issue: "4"; pages: 
            "1169-1181"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (27-JAN-2000) Chris M. Brown, University of Otago, Department of 
            Biochemistry"; volume: " P.O.Box 56, Dunedin, Otago 9001, New 
            Zealand (E-mail:chris.brown@stonebow.otago.ac.nz, 
            Tel:+64-3-479-7875, Fax"; pages: "+64-3-479-7866"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5917
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..495
                     /label=HBV Adw 5' leader of pregenomic RNA
                     /note="HBV Adw 5' leader of pregenomic RNA"
     CDS             35..94
                     /codon_start=1
                     /gene="C0"
                     /label=C0
                     /note="unnamed protein product; ORF"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /protein_id="BAA93573.1"
                     /translation="MSHCSSLQAVPWVALGHGH"
     gene            35..94
                     /gene="C0"
                     /label=C0
     CDS             84..581
                     /codon_start=1
                     /product="modified C"
                     /label=modified C
                     /protein_id="BAA93574.1"
                     /translation="MDIDPYKEFGATVELLSFLPSDFFPSVRDLLDTASALYREALESP
                     EHCSPHHTALRQAILCWGELMTLATWVGNNLEDPATRDLVVNYVNTNMGLKIRQLLWFH
                     ISCLTFGRETVLEYLVSFGVWIRTPPAYRPPNARRRRRQKHKERPGAILSAGRWNRWRA
                     TA"
     CDS             499..2148
                     /codon_start=1
                     /label=luciferase
                     /note="firefly luciferase"
                     /translation="VEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    complement(2361..2482)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3147..3735)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3909..4766)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4767..4871)
                     /label=AmpR promoter
     rep_origin      4898..5353
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    5484..5532
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    5546..5637
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
     promoter        5696..5892
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        5899..5917
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 aactttttca cctctgccta atcatctctt gtacatgtcc cactgttcaa gcctccaagc
       61 tgtgccttgg gtggctttgg ggcatggaca ttgaccctta taaagaattt ggagctactg
      121 tggagttact ctcgtttttg ccttctgact tctttccttc cgtcagagat ctcctagaca
      181 ccgcctcagc tctgtatcgg gaagccttag agtctcctga gcattgctca cctcaccaca
      241 ccgcactcag gcaagccatt ctctgctggg gggaattgat gactctagct acctgggtgg
      301 gtaataattt ggaagatcca gcaactaggg atctagtagt caattatgtt aatactaaca
      361 tgggtttaaa aattaggcaa ctattgtggt ttcatatatc ttgccttact tttggaagag
      421 agactgtact tgaatatttg gtatctttcg gagtgtggat tcgcactcct ccagcctata
      481 gaccaccaaa tgcccgacgt cgaagacgcc aaaaacataa agaaaggccc ggcgccattc
      541 tatccgctgg aagatggaac cgctggagag caactgcata aggctatgaa gagatacgcc
      601 ctggttcctg gaacaattgc ttttacagat gcacatatcg aggtggacat cacttacgct
      661 gagtacttcg aaatgtccgt tcggttggca gaagctatga aacgatatgg gctgaataca
      721 aatcacagaa tcgtcgtatg cagtgaaaac tctcttcaat tctttatgcc ggtgttgggc
      781 gcgttattta tcggagttgc agttgcgccc gcgaacgaca tttataatga acgtgaattg
      841 ctcaacagta tgggcatttc gcagcctacc gtggtgttcg tttccaaaaa ggggttgcaa
      901 aaaattttga acgtgcaaaa aaagctccca atcatccaaa aaattattat catggattct
      961 aaaacggatt accagggatt tcagtcgatg tacacgttcg tcacatctca tctacctccc
     1021 ggttttaatg aatacgattt tgtgccagag tccttcgata gggacaagac aattgcactg
     1081 atcatgaact cctctggatc tactggtctg cctaaaggtg tcgctctgcc tcatagaact
     1141 gcctgcgtga gattctcgca tgccagagat cctatttttg gcaatcaaat cattccggat
     1201 actgcgattt taagtgttgt tccattccat cacggttttg gaatgtttac tacactcgga
     1261 tatttgatat gtggatttcg agtcgtctta atgtatagat ttgaagaaga gctgtttctg
     1321 aggagccttc aggattacaa gattcaaagt gcgctgctgg tgccaaccct attctccttc
     1381 ttcgccaaaa gcactctgat tgacaaatac gatttatcta atttacacga aattgcttct
     1441 ggtggcgctc ccctctctaa ggaagtcggg gaagcggttg ccaagaggtt ccatctgcca
     1501 ggtatcaggc aaggatatgg gctcactgag actacatcag ctattctgat tacacccgag
     1561 ggggatgata aaccgggcgc ggtcggtaaa gttgttccat tttttgaagc gaaggttgtg
     1621 gatctggata ccgggaaaac gctgggcgtt aatcaaagag gcgaactgtg tgtgagaggt
     1681 cctatgatta tgtccggtta tgtaaacaat ccggaagcga ccaacgcctt gattgacaag
     1741 gatggatggc tacattctgg agacatagct tactgggacg aagacgaaca cttcttcatc
     1801 gttgaccgcc tgaagtctct gattaagtac aaaggctatc aggtggctcc cgctgaattg
     1861 gaatccatct tgctccaaca ccccaacatc ttcgacgcag gtgtcgcagg tcttcccgac
     1921 gatgacgccg gtgaacttcc cgccgccgtt gttgttttgg agcacggaaa gacgatgacg
     1981 gaaaaagaga tcgtggatta cgtcgccagt caagtaacaa ccgcgaaaaa gttgcgcgga
     2041 ggagttgtgt ttgtggacga agtaccgaaa ggtcttaccg gaaaactcga cgcaagaaaa
     2101 atcagagaga tcctcataaa ggccaagaag ggcggaaaga tcgccgtgta attctagaaa
     2161 ctttttcacc tctgcctaat catctcttgt acatgtccca ctgttcaagc ctccaagctg
     2221 tgccttgggt ggctttgggg catggacatt gacccttaaa agaatttgga gctacaaaaa
     2281 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaagaa ttctagagtc ggggcggccg
     2341 gccgcttcga gcagacatga taagatacat tgatgagttt ggacaaacca caactagaat
     2401 gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat
     2461 tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca
     2521 gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtaaaatcga
     2581 taaggatctg aacgatggag cggagaatgg gcggaactgg gcggagttag gggcgggatg
     2641 ggcggagtta ggggcgggac tatggttgct gactaattga gatgcatgct ttgcatactt
     2701 ctgcctgctg gggagcctgg ggactttcca cacctggttg ctgactaatt gagatgcatg
     2761 ctttgcatac ttctgcctgc tggggagcct ggggactttc cacaccctaa ctgacacaca
     2821 ttccacagcg gatccgtcga ccgatgccct tgagagcctt caacccagtc agctccttcc
     2881 ggtgggcgcg gggcatgact atcgtcgccg cacttatgac tgtcttcttt atcatgcaac
     2941 tcgtaggaca ggtgccggca gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     3001 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     3061 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     3121 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     3181 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     3241 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     3301 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat
     3361 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     3421 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     3481 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     3541 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     3601 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     3661 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     3721 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     3781 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     3841 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     3901 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     3961 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     4021 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     4081 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     4141 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     4201 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     4261 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     4321 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     4381 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     4441 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     4501 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     4561 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     4621 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     4681 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     4741 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     4801 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     4861 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg cgccctgtag cggcgcatta
     4921 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     4981 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     5041 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     5101 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     5161 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     5221 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     5281 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     5341 acgtttacaa tttcccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
     5401 cgggcctctt cgctattacg ccagcccaag ctaccatgat aagtaagtaa tattaaggta
     5461 cgggaggtac ttggagcggc cgcaataaaa tatctttatt ttcattacat ctgtgtgttg
     5521 gttttttgtg tgaatcgata gtactaacat acgctctcca tcaaaacaaa acgaaacaaa
     5581 acaaactagc aaaataggct gtccccagtg caagtgcagg tgccagaaca tttctctatc
     5641 gataggtacc gagctcttac gcgtgctagc ccgggctcga gatcgatctg cgatctgcat
     5701 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     5761 cccagttccg cccattctcc gccccatcgc tgactaattt tttttattta tgcagaggcc
     5821 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     5881 ggcttttgca aaaagcttta atacgactca ctatagg
//
