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pPLc236 vector (V004035)

Basic Vector Information

Vector Name:
pPLc236
Antibiotic Resistance:
Ampicillin
Length:
4425 bp
Type:
Bacterial expression vector
Replication origin:
ori
Source/Author:
De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman Fonseca M, Vanhoucke M, Beyaert R.

pPLc236 vector Map

Copy Sequence View Map
pPLc2364425 bp600120018002400300036004200ropbomoriAmpRPL

pPLc236 vector Sequence

LOCUS       40924_34934        4425 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Bacterial expression vector pPLc236, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4425)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 4425)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4425)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4425
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1575..1763
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    1868..2008
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(2194..2782)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2956..3813)
                     /label=AmpR
                     /note="beta-lactamase"
     regulatory      4179..4309
                     /label=PL
                     /note="PL"
                     /regulatory_class="promoter"

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