LOCUS       40924_34934        4425 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Bacterial expression vector pPLc236, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4425)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 4425)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4425)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4425
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1575..1763
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    1868..2008
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(2194..2782)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2956..3813)
                     /label=AmpR
                     /note="beta-lactamase"
     regulatory      4179..4309
                     /label=PL
                     /note="PL"
                     /regulatory_class="promoter"
ORIGIN
        1 ttccggatcc ggccaagctt ggccaagctt ggccggatcc tctacgccgg acgcatcgtg
       61 gccggcatca ccggcgccac aggtgcggtt gctggcgcct atatcgccga catcaccgat
      121 ggggaagatc gggctcgcca cttcgggctc atgagcgctt gtttcggcgt gggtatggtg
      181 gcaggccccg tggccggggg actgttgggc gccatctcct tgcatgcacc attccttgcg
      241 gcggcggtgc tcaacggcct caacctacta ctgggctgct tcctaatgca ggagtcgcat
      301 aagggagagc gtcgaccgat gcccttgaga gccttcaacc cagtcagctc cttccggtgg
      361 gcgcggggca tgactatcgt cgccgcactt atgactgtct tctttatcat gcaactcgta
      421 ggacaggtgc cggcagcgct ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg
      481 acgatgatcg gcctgtcgct tgcggtattc ggaatcttgc acgccctcgc tcaagccttc
      541 gtcactggtc ccgccaccaa acgtttcggc gagaagcagg ccattatcgc cggcatggcg
      601 gccgacgcgc tgggctacgt cttgctggcg ttcgcgacgc gaggctggat ggccttcccc
      661 attatgattc ttctcgcttc cggcggcatc gggatgcccg cgttgcaggc catgctgtcc
      721 aggcaggtag atgacgacca tcagggacag cttcaaggat cgctcgcggc tcttaccagc
      781 ctaacttcga tcactggacc gctgatcgtc acggcgattt atgccgcctc ggcgagcaca
      841 tggaacgggt tggcatggat tgtaggcgcc gccctatacc ttgtctgcct ccccgcgttg
      901 cgtcgcggtg catggagccg ggccacctcg acctgaatgg aagccggcgg cacctcgcta
      961 acggattcac cactccaaga attggagcca atcaattctt gcggagaact gtgaatgcgc
     1021 aaaccaaccc ttggcagaac atatccatcg cgtccgccat ctccagcagc cgcacgcggc
     1081 gcatctcggg cagcgttggg tcctggccac gggtgcgcat gatcgtgctc ctgtcgttga
     1141 ggacccggct aggctggcgg ggttgcctta ctggttagca gaatgaatca ccgatacgcg
     1201 agcgaacgtg aagcgactgc tgctgcaaaa cgtctgcgac ctgagcaaca acatgaatgg
     1261 tcttcggttt ccgtgtttcg taaagtctgg aaacgcggaa gtcagcgccc tgcaccatta
     1321 tgttccggat ctgcatcgca ggatgctgct ggctaccctg tggaacacct acatctgtat
     1381 taacgaagcg ctggcattga ccctgagtga tttttctctg gtcccgccgc atccataccg
     1441 ccagttgttt accctcacaa cgttccagta accgggcatg ttcatcatca gtaacccgta
     1501 tcgtgagcat cctctctcgt ttcatcggta tcattacccc catgaacaga aatccccctt
     1561 acacggaggc atcagtgacc aaacaggaaa aaaccgccct taacatggcc cgctttatca
     1621 gaagccagac attaacgctt ctggagaaac tcaacgagct ggacgcggat gaacaggcag
     1681 acatctgtga atcgcttcac gaccacgctg atgagcttta ccgcagctgc ctcgcgcgtt
     1741 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
     1801 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
     1861 gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta
     1921 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag
     1981 atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct
     2041 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     2101 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     2161 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     2221 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     2281 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     2341 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     2401 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     2461 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     2521 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     2581 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
     2641 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     2701 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     2761 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     2821 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     2881 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     2941 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     3001 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     3061 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     3121 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     3181 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     3241 tagtttgcgc aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg
     3301 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     3361 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     3421 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     3481 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     3541 gcgaccgagt tgctcttgcc cggcgtcaac acgggataat accgcgccac atagcagaac
     3601 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     3661 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     3721 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     3781 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attatgtaag
     3841 cagacagttt tattgttcat gatgatatat ttttatcttg tgcaatgtaa catcagagat
     3901 tttgagacac aacgtggctt tgttgaataa atcgaacttt tgctgagttg aaggatcaga
     3961 tcacgcatct tcccgacaac gcagaccgtt ccgtggcaaa gcaaaagttc aaaatcacca
     4021 actggtccac ctacaacaaa gctctcatca accgtggctc cctcactttc tggctggatg
     4081 atggggcgat tcaggcctgg tatgagtcag caacaccttc ttcacgaggc agacctcagc
     4141 gccggtgatg ccggccacga tgcgtccggc gtagaggatc tctcacctac caaacaatgc
     4201 ccccctgcaa aaaataaatt catataaaaa acatacagat aaccatctgc ggtgataaat
     4261 tatctctggc ggtgttgaca taaataccac tggcggtgat actgagcaca tcagcaggac
     4321 gcactgacca ccatgaaggt gacgctctta aaattaagcc ctgaagaagg gcagcattca
     4381 aagcagaagg ctttggggtg tgtgatacga aacgaagcat tggaa
//
