pPLaACR26 vector (V004039)

LOCUS       V004039                 6783 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004039
VERSION     V004039
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6783)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6783)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 6783)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6783)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6783
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      complement(117..247)
                     /label="PL promoter"
                     /note="PL promoter"
                     /regulatory_class="promoter"
     CDS             615..1427
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      complement(1889..2477)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(2965..3154)
                     /label="insertion element 1"
                     /note="insertion element 1"
     CDS             complement(3491..5128)
                     /codon_start=1
                     /note="unnamed protein product; POL"
                     /protein_id="SJL88457.1"
                     /translation="MSKTTKKFNSLCIDLPRDLSLEIYQSIASVATGSGDPHSDDFTAI
                     AYLRDELLTKHPTLGSGNDEATRRTLAIAKLREANGDRGQINREGFLHDKSLSWDPDVL
                     QTSIRSLIGNLLSGYRSSLFGQCTFSNGAPMGHKLQDAAPYKKFAEQATVTPRALRAAL
                     LVRDQCAPWIRHAVRYNESYEFRLVVGNGVFTVPKNNKIDRAACKEPDMNMYLQKGVGA
                     FIRRRLKSVGIDLNDQSINQRLAQQGSVDGSLATIDLSSASDSISDRLVWSFLPPELYS
                     YLDRIRSHYGIVDGETIRWELFSTMGNGFTFELESMIFWAIVKATQIHFGNAGTIGIYG
                     DDIICPSEIAPRVLEALAYYGFKPNLRKTFVSGLFRESCGAHFYRGVDVKPFYIKKPVD
                     NLFALMLILNRLRGWGVVGGMSDPRLYKVWVRLSSQVPSMFFGGTDLAADYYVVSPPTA
                     VSVYTKTPYGRLLADTRTSGFRLARIARERKFFSEKHDSGRYIAWFHTGGEITDSMKSA
                     GVRVIRTSEWLTPVPTFPQECGPASSPR"
     misc_feature    complement(5129..5161)
                     /label="5' UTR of POL"
                     /note="5' UTR of POL"
     CDS             complement(5165..5551)
                     /label="MS2"
                     /note="bacteriophage MS2 coat protein"
     misc_feature    complement(5555..5577)
                     /label="5' UTR of COAT"
                     /note="5' UTR of COAT"
     CDS             complement(5581..6759)
                     /gene="A"
                     /label="Maturation protein A"
                     /note="Maturation protein A from Escherichia phage MS2.
                     Accession#: P03610"
     misc_feature    complement(6760..6783)
                     /label="5' UTR of A"
                     /note="5' UTR of A"