LOCUS       V004039                 6783 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004039
VERSION     V004039
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6783)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6783)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 6783)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6783)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6783
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      complement(117..247)
                     /label="PL promoter"
                     /note="PL promoter"
                     /regulatory_class="promoter"
     CDS             615..1427
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      complement(1889..2477)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(2965..3154)
                     /label="insertion element 1"
                     /note="insertion element 1"
     CDS             complement(3491..5128)
                     /codon_start=1
                     /note="unnamed protein product; POL"
                     /protein_id="SJL88457.1"
                     /translation="MSKTTKKFNSLCIDLPRDLSLEIYQSIASVATGSGDPHSDDFTAI
                     AYLRDELLTKHPTLGSGNDEATRRTLAIAKLREANGDRGQINREGFLHDKSLSWDPDVL
                     QTSIRSLIGNLLSGYRSSLFGQCTFSNGAPMGHKLQDAAPYKKFAEQATVTPRALRAAL
                     LVRDQCAPWIRHAVRYNESYEFRLVVGNGVFTVPKNNKIDRAACKEPDMNMYLQKGVGA
                     FIRRRLKSVGIDLNDQSINQRLAQQGSVDGSLATIDLSSASDSISDRLVWSFLPPELYS
                     YLDRIRSHYGIVDGETIRWELFSTMGNGFTFELESMIFWAIVKATQIHFGNAGTIGIYG
                     DDIICPSEIAPRVLEALAYYGFKPNLRKTFVSGLFRESCGAHFYRGVDVKPFYIKKPVD
                     NLFALMLILNRLRGWGVVGGMSDPRLYKVWVRLSSQVPSMFFGGTDLAADYYVVSPPTA
                     VSVYTKTPYGRLLADTRTSGFRLARIARERKFFSEKHDSGRYIAWFHTGGEITDSMKSA
                     GVRVIRTSEWLTPVPTFPQECGPASSPR"
     misc_feature    complement(5129..5161)
                     /label="5' UTR of POL"
                     /note="5' UTR of POL"
     CDS             complement(5165..5551)
                     /label="MS2"
                     /note="bacteriophage MS2 coat protein"
     misc_feature    complement(5555..5577)
                     /label="5' UTR of COAT"
                     /note="5' UTR of COAT"
     CDS             complement(5581..6759)
                     /gene="A"
                     /label="Maturation protein A"
                     /note="Maturation protein A from Escherichia phage MS2.
                     Accession#: P03610"
     misc_feature    complement(6760..6783)
                     /label="5' UTR of A"
                     /note="5' UTR of A"
ORIGIN
        1 ttccaatgct tcgtttcgta tcacacaccc caaagccttc tgctttgaat gctgcccttc
       61 ttcagggctt aattttaaga gcgtcacctt catggtggtc agtgcgtcct gctgatgtgc
      121 tcagtatcac cgccagtggt atttatgtca acaccgccag agataattta tcaccgcaga
      181 tggttatctg tatgtttttt atatgaattt attttttgca ggggggcatt gtttggtagg
      241 tgagagatcc tctacgccgg acgcatcgtg gccggcatca ccggcgctga ggtctgcctc
      301 gtgaagaagg tgttgctgac tcataccagg cctgaatcgc cccatcatcc agccagaaag
      361 tgagggagcc acggttgatg agagctttgt tgtaggtgga ccagttggtg attttgaact
      421 tttgctttgc cacggaacgg tctgcgttgt cgggaagatg cgtgatctga tccttcaact
      481 cagcaaaagt tcgatttatt caacaaagcc acgttgtgtc tcaaaatctc tgatgttaca
      541 ttgcacaaga taaaaatata tcatcatgaa caataaaact gtctgcttac ataaacagta
      601 atacaagggg tgttatgagc catattcaac gggaaacgtc ttgctcgagg ccgcgattaa
      661 attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat gtcgggcaat
      721 caggtgcgac aatctatcga ttgtatggga agcccgatgc gccagagttg tttctgaaac
      781 atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta aactggctga
      841 cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat gatgcatggt
      901 tactcaccac tgcgatcccc gggaaaacag cattccaggt attagaagaa tatcctgatt
      961 caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat tcgattcctg
     1021 tttgtaattg tccttttaac agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa
     1081 tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg
     1141 aacaagtctg gaaagaaatg cataagcttt tgccattctc accggattca gtcgtcactc
     1201 atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata ggttgtattg
     1261 atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta tggaactgcc
     1321 tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt attgataatc
     1381 ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa tcagaattgg
     1441 ttaattggtt gtaacactgg cagagcatta cgctgacttg acgggacggc ggctttgttg
     1501 aataaatcga acttttgctg agttgaagga tcagatcacg catcttcccg acaacgcaga
     1561 ccgttccgtg gcaaagcaaa agttcaaaat caccaactgg tccacctaca acaaagctct
     1621 catcaaccgt ggctccctca ctttctggct ggatgatggg gcgattcagg cctggtatga
     1681 gtcagcaaca ccttcttcac gaggcagacc tcagcgcttt tccgcttcct cgctcactga
     1741 ctcgctacgc tcggtcgttc gactgcggcg agcggtactg actcacacaa aaacggtaac
     1801 acagttatcc acagaatcag gggataaggc cggaaagaac atgtgagcaa aagaccagga
     1861 acaggaagaa ggccacgtag caggcgtttt tccataggct ccgcccccct gacgagcatc
     1921 acaaaaatag acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     1981 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     2041 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     2101 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     2161 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     2221 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     2281 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     2341 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     2401 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     2461 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     2521 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     2581 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     2641 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     2701 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     2761 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     2821 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     2881 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     2941 tgcgcaacgt tgttgccatt gctgcagttg ccatgtttta cggcagtgag agcagagata
     3001 gcgctgatgt ccggcggtgc ttttgccgtt acgcaccacc ccgtcagtag ctgaacagga
     3061 gggacagctg atagaaacag aagccactgg agcacctcaa aaacaccatc atacactaaa
     3121 tcagtaagtt ggcagcatca cccacgatgc ctgcaaaaaa aaaaaaaaaa aaaaaaaaaa
     3181 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     3241 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     3301 aaaaattttt tttttttttt gggtggtaac tagccaagca gctagttacc aaatcgggag
     3361 aatcccgggt cctctcttta gggggaggtc cctgggccga agcccgccca cctttcggtg
     3421 gagccggacc gctttcgcac ccgtgctctt tcgagcacac ccaccccgtt tacgggggtc
     3481 cctcggtcag ctaccgagga gagctcgctg gcccacactc ctgagggaat gtgggaaccg
     3541 gcgttagcca ctccgaagtg cgtataacgc gcacgccggc ggacttcatg ctgtcggtga
     3601 tttcacctcc agtatggaac cacgctatgt agcgaccact gtcgtgcttt tcgctgaaga
     3661 acttgcgttc tcgagcgata cgagcaagac ggaaacccga ggtacgggta tccgcgagca
     3721 gccgcccgta cggagtcttg gtgtataccg agactgccgt aggcgggctg actacgtagt
     3781 agtcggcagc gaggtccgtc ccaccgaaga acatcgaagg cacctgggag gagagccgta
     3841 cccacacctt atagaggcgt ggatctgaca tacctccgac aactccccaa ccccgtagcc
     3901 gatttaatat cagcatcagg gcgaagagat tgtcaacagg tttcttgatg taaaacggtt
     3961 tgacatcgac accacggtaa aagtgcgcgc cgcagctctc gcgaaagagc ccggacacga
     4021 acgttttacg aagattcggt ttaaaaccgt agtaggcaag tgcctctagc acacggggtg
     4081 caatctcact gggacatata atatcgtccc cgtagatgcc tatggttccg gcgttaccaa
     4141 aatggatttg ggtcgctttg actattgccc agaatatcat ggactctagc tcaaatgtga
     4201 acccatttcc cattgtggaa aatagttccc atcgtatcgt ctcgccatct acgattccgt
     4261 agtgtgagcg gatacgatcg agatatgaat atagctcagg tgggagaaaa ctccacacca
     4321 ggcgatcgga gatggaatcg gatgcagacg ataagtctat cgtcgcaagc gaaccatcta
     4381 cgctgccctg ctgagccaga cgctggttga tcgattgatc attcaggtct ataccaacgg
     4441 atttgagccg gcgtctgatg aaagcaccga cccctttctg gaggtacata ttcatatcag
     4501 gctccttaca ggcagcccga tctattttat tattcttcgg aactgtaaac actccgttcc
     4561 ctacaacgag cctaaattca tatgactcgt tatagcggac cgcgtgtctg atccacggcg
     4621 cacattggtc tcggaccaat agagccgctc tcagagcgcg gggggtaacg gttgcttgtt
     4681 cagcgaactt cttgtaaggc gctgcatcct gcaacttgtg ccccatagga gcaccgttgg
     4741 agaacgtgca ttgcccaaac aacgacgatc ggtagccaga gaggaggttg ccaataaggc
     4801 tacggatgct ggtttgtaaa acatccggat cccatgacaa ggatttgtca tgtaagaaac
     4861 cttctctatt tatctgaccg cgatcaccat tcgcctcccg tagcttagcg atagctaagg
     4921 tacgacgggt cgcctcgtca ttaccagaac ctaaggtcgg atgctttgtg agcaattcgt
     4981 cccttaagta agcaattgct gtaaagtcgt cactgtgcgg atcaccgctt ccagtagcga
     5041 cagaagcaat tgattggtaa atttcgagag aaagatcgcg aggaagatca atacataaag
     5101 agttgaactt ctttgttgtc ttcgacatgg gtaatcctca tgtttgaatg gccggcgtct
     5161 attagtagat gccggagttt gctgcgattg ctgagggaat cgggtttcca tcttttagga
     5221 gaccttgcat tgccttaaca ataagctcgc agtcggaatt cgtagcgaaa attggaatgg
     5281 ttagttccat atttaagtac gaacgccatg cggctacagg aagctctaca ccaccaacag
     5341 tctgggttgc cactttaggc acctcgactt tgatggtgta tttgcgattc tgcgcagagc
     5401 tctgacgaac gctacaggtt actttgtaag cctgtgaacg cgagttagag ctgatccatt
     5461 cagcgacccc gttagcgaag ttgcttgggg cgacagtcac gtcgccagtt ccgccattgt
     5521 cgacgagaac gaactgagta aagttagaag ccatgcttca aactccggtt gagggctcta
     5581 tctagagagc cgttgcctga ttaatgctaa cgcatctaag gtatggacca tcgagaaagg
     5641 agactttacg tacgcgccag ttgttggcca tacggattgt acccctcgat gcatggctga
     5701 gatttgggcc ttagcagtgc cctgtctctc cacagtccac ccgtagggag cgtcaacgct
     5761 tatgatggac tcacccgtta ttacgtcagt aactgttcct gacatgtagg agcatcccac
     5821 gggggccgta aggccctcga gcatgttacc tacaggtagg agccagtcga caacgaatga
     5881 gaaaggcacc ttttcccaca ctatacctag tgggttcaag atacctagag acgacaacca
     5941 tgccaaacgt gcatcgttta tgtaaaacca tatcacgata cgtcgcgata tgttgcacgt
     6001 tgtctggaag tttgcagctg gatacgacag acggccatct aacttgatgt tagtaccgac
     6061 ctgacgtacg gctctcatag gaagaaactc ttgaaggtga accttcgtaa gcatctcata
     6121 tgcaccctgg atatcactca ttagtggtaa ccaaccgaac tgcaactcca accacctgcc
     6181 ggccacgtgt tttgatcgaa actttcgatc ttcgtttagg gcaaggtagc ggagcgcctg
     6241 gcgccaatta ccgcgacgag cggcagtgta cgccttcacg agcgcaatgg tttgcgtcgc
     6301 gagttgtgag gctgtcgacc tggcctctgc taaagcaaca ccaaggttaa aattaccctg
     6361 ggtgaccttt tgcaggactt cggtcgacgc ccggttcgca acgttctgcg gcacttcgat
     6421 gtaagtcaag ttttggctta cagggaagag gctgtagcag gagcgtgcgt cgagggagaa
     6481 gccgaaaccg gctttctcct cgtacgggcg accccacgat gacccacttc gcttgtaggc
     6541 accttgatct atcgatgtga cacttaacgc cccccgtgaa tacggagagg ggtagtgcca
     6601 ctgtttcgtt ttggccccag tcgagttaaa acgaccggga gtccagttcg aacgatattt
     6661 taaagagaat gagttatctt cagtctcacc gtccgcgtaa acgcgaacgg aggggacgaa
     6721 ggtctcgttc tccctatcaa gggtactaaa agctcgcaca ggtcaaacct cctaggaatg
     6781 gaa
//
