Basic Vector Information
- Vector Name:
- pPLa8
- Length:
- 3716 bp
- Type:
- Bacterial expression vector
- Replication origin:
- ori
- Source/Author:
- De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman Fonseca M, Vanhoucke M, Beyaert R.
pPLa8 vector Map
pPLa8 vector Sequence
LOCUS 40924_34904 3716 bp DNA circular SYN 18-DEC-2018
DEFINITION Bacterial expression vector pPLa8, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3716)
AUTHORS De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
Fonseca M, Vanhoucke M, Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 3716)
AUTHORS De Schamphelaire W.
TITLE Direct Submission
JOURNAL Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM
REFERENCE 3 (bases 1 to 3716)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 3716)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
9052, BELGIUM"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..3716
/mol_type="other DNA"
/organism="synthetic DNA construct"
regulatory complement(117..247)
/label=PL promoter
/note="PL promoter"
/regulatory_class="promoter"
CDS 615..1427
/label=KanR
/note="aminoglycoside phosphotransferase"
rep_origin complement(1889..2477)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(2651..3508)
/label=AmpR
/note="beta-lactamase"
promoter complement(3509..3613)
/label=AmpR promoter
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