LOCUS       40924_34904        3716 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Bacterial expression vector pPLa8, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3716)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3716)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 3716)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3716)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3716
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      complement(117..247)
                     /label=PL promoter
                     /note="PL promoter"
                     /regulatory_class="promoter"
     CDS             615..1427
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      complement(1889..2477)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2651..3508)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3509..3613)
                     /label=AmpR promoter
ORIGIN
        1 ttccaatgct tcgtttcgta tcacacaccc caaagccttc tgctttgaat gctgcccttc
       61 ttcagggctt aattttaaga gcgtcacctt catggtggtc agtgcgtcct gctgatgtgc
      121 tcagtatcac cgccagtggt atttatgtca acaccgccag agataattta tcaccgcaga
      181 tggttatctg tatgtttttt atatgaattt attttttgca ggggggcatt gtttggtagg
      241 tgagagatcc tctacgccgg acgcatcgtg gccggcatca ccggcgctga ggtctgcctc
      301 gtgaagaagg tgttgctgac tcataccagg cctgaatcgc cccatcatcc agccagaaag
      361 tgagggagcc acggttgatg agagctttgt tgtaggtgga ccagttggtg attttgaact
      421 tttgctttgc cacggaacgg tctgcgttgt cgggaagatg cgtgatctga tccttcaact
      481 cagcaaaagt tcgatttatt caacaaagcc acgttgtgtc tcaaaatctc tgatgttaca
      541 ttgcacaaga taaaaatata tcatcatgaa caataaaact gtctgcttac ataaacagta
      601 atacaagggg tgttatgagc catattcaac gggaaacgtc ttgctcgagg ccgcgattaa
      661 attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat gtcgggcaat
      721 caggtgcgac aatctatcga ttgtatggga agcccgatgc gccagagttg tttctgaaac
      781 atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta aactggctga
      841 cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat gatgcatggt
      901 tactcaccac tgcgatcccc gggaaaacag cattccaggt attagaagaa tatcctgatt
      961 caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat tcgattcctg
     1021 tttgtaattg tccttttaac agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa
     1081 tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg
     1141 aacaagtctg gaaagaaatg cataagcttt tgccattctc accggattca gtcgtcactc
     1201 atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata ggttgtattg
     1261 atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta tggaactgcc
     1321 tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt attgataatc
     1381 ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa tcagaattgg
     1441 ttaattggtt gtaacactgg cagagcatta cgctgacttg acgggacggc ggctttgttg
     1501 aataaatcga acttttgctg agttgaagga tcagatcacg catcttcccg acaacgcaga
     1561 ccgttccgtg gcaaagcaaa agttcaaaat caccaactgg tccacctaca acaaagctct
     1621 catcaaccgt ggctccctca ctttctggct ggatgatggg gcgattcagg cctggtatga
     1681 gtcagcaaca ccttcttcac gaggcagacc tcagcgcttt tccgcttcct cgctcactga
     1741 ctcgctacgc tcggtcgttc gactgcggcg agcggtactg actcacacaa aaacggtaac
     1801 acagttatcc acagaatcag gggataaggc cggaaagaac atgtgagcaa aagaccagga
     1861 acaggaagaa ggccacgtag caggcgtttt tccataggct ccgcccccct gacgagcatc
     1921 acaaaaatag acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     1981 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     2041 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     2101 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     2161 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     2221 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     2281 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     2341 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     2401 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     2461 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     2521 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     2581 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     2641 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     2701 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     2761 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     2821 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     2881 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     2941 tgcgcaacgt tgttgccatc cggatccgga tcgtggtgtc acgctcgtcg tttggtatgg
     3001 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     3061 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     3121 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     3181 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     3241 cgagttgctc ttgcccggcg tcaacacggg ataataccgc gccacatagc agaactttaa
     3301 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     3361 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     3421 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     3481 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     3541 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     3601 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta
     3661 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctt caagaa
//
