pPLa2311trpA1 vector (V004042)

LOCUS       V004042                 9058 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004042
VERSION     V004042
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9058)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9058)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 9058)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9058)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9058
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             615..1427
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      complement(1889..2477)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2651..3508)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3509..3613)
                     /label="AmpR promoter"
     CDS             complement(7095..7898)
                     /gene="trpA"
                     /label="Tryptophan synthase alpha chain"
                     /note="Tryptophan synthase alpha chain from Salmonella
                     typhimurium (strain LT2 / SGSC1412 / ATCC 700720).
                     Accession#: P00929"
     CDS             complement(join(7901..9058,1..3))
                     /gene="trpB"
                     /label="Tryptophan synthase beta chain"
                     /note="Tryptophan synthase beta chain from Salmonella
                     typhimurium (strain LT2 / SGSC1412 / ATCC 700720).
                     Accession#: P0A2K1"