LOCUS       V004042                 9058 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004042
VERSION     V004042
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9058)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9058)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 9058)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9058)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9058
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             615..1427
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      complement(1889..2477)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2651..3508)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3509..3613)
                     /label="AmpR promoter"
     CDS             complement(7095..7898)
                     /gene="trpA"
                     /label="Tryptophan synthase alpha chain"
                     /note="Tryptophan synthase alpha chain from Salmonella
                     typhimurium (strain LT2 / SGSC1412 / ATCC 700720).
                     Accession#: P00929"
     CDS             complement(join(7901..9058,1..3))
                     /gene="trpB"
                     /label="Tryptophan synthase beta chain"
                     /note="Tryptophan synthase beta chain from Salmonella
                     typhimurium (strain LT2 / SGSC1412 / ATCC 700720).
                     Accession#: P0A2K1"
ORIGIN
        1 ttccaatgct tcgtttcgta tcacacaccc caaagccttc tgctttgaat gctgcccttc
       61 ttcagggctt aattttaaga gcgtcacctt catggtggtc agtgcgtcct gctgatgtgc
      121 tcagtatcac cgccagtggt atttatgtca acaccgccag agataattta tcaccgcaga
      181 tggttatctg tatgtttttt atatgaattt attttttgca ggggggcatt gtttggtagg
      241 tgagagatcc tctacgccgg acgcatcgtg gccggcatca ccggcgctga ggtctgcctc
      301 gtgaagaagg tgttgctgac tcataccagg cctgaatcgc cccatcatcc agccagaaag
      361 tgagggagcc acggttgatg agagctttgt tgtaggtgga ccagttggtg attttgaact
      421 tttgctttgc cacggaacgg tctgcgttgt cgggaagatg cgtgatctga tccttcaact
      481 cagcaaaagt tcgatttatt caacaaagcc acgttgtgtc tcaaaatctc tgatgttaca
      541 ttgcacaaga taaaaatata tcatcatgaa caataaaact gtctgcttac ataaacagta
      601 atacaagggg tgttatgagc catattcaac gggaaacgtc ttgctcgagg ccgcgattaa
      661 attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat gtcgggcaat
      721 caggtgcgac aatctatcga ttgtatggga agcccgatgc gccagagttg tttctgaaac
      781 atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta aactggctga
      841 cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat gatgcatggt
      901 tactcaccac tgcgatcccc gggaaaacag cattccaggt attagaagaa tatcctgatt
      961 caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat tcgattcctg
     1021 tttgtaattg tccttttaac agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa
     1081 tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg
     1141 aacaagtctg gaaagaaatg cataagcttt tgccattctc accggattca gtcgtcactc
     1201 atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata ggttgtattg
     1261 atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta tggaactgcc
     1321 tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt attgataatc
     1381 ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa tcagaattgg
     1441 ttaattggtt gtaacactgg cagagcatta cgctgacttg acgggacggc ggctttgttg
     1501 aataaatcga acttttgctg agttgaagga tcagatcacg catcttcccg acaacgcaga
     1561 ccgttccgtg gcaaagcaaa agttcaaaat caccaactgg tccacctaca acaaagctct
     1621 catcaaccgt ggctccctca ctttctggct ggatgatggg gcgattcagg cctggtatga
     1681 gtcagcaaca ccttcttcac gaggcagacc tcagcgcttt tccgcttcct cgctcactga
     1741 ctcgctacgc tcggtcgttc gactgcggcg agcggtactg actcacacaa aaacggtaac
     1801 acagttatcc acagaatcag gggataaggc cggaaagaac atgtgagcaa aagaccagga
     1861 acaggaagaa ggccacgtag caggcgtttt tccataggct ccgcccccct gacgagcatc
     1921 acaaaaatag acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     1981 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     2041 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     2101 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     2161 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     2221 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     2281 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     2341 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     2401 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     2461 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     2521 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     2581 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     2641 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     2701 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     2761 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     2821 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     2881 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     2941 tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg
     3001 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     3061 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     3121 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     3181 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     3241 cgagttgctc ttgcccggcg tcaacacggg ataataccgc gccacatagc agaactttaa
     3301 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     3361 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     3421 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     3481 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     3541 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     3601 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta
     3661 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctt caagaattct
     3721 ttatgcgtgc aggtccggca accgtcagac ctacagaagg ggcgggtggt acgctggggc
     3781 atttaaacgg gtttgatgtg agtaataaca cgcaactcgg cctgacattc acttacatgg
     3841 cgacggacaa tatcggggtt gaattgctgg cggctacgcc gttccgccat aaggtgggta
     3901 cgggcgcgac gggcgatatc gccacggtcc acctgttacc gccaacgtta atggcgcagt
     3961 ggtacttcgg cgattccagc agcaaagtgc gtccttatgt cggcgtgggg gtgaactaca
     4021 ccaccttctt cgataacgac tttaacgata atggtaaaaa tgccgggctg tccgatctga
     4081 gctttaagga ttcctggggc gcggcagggc aagttggggt agattacctg attaaccgcg
     4141 actggttaat tggcgcctct gtctggtaca tggatattga tactaccgcc aattataaaa
     4201 tgggcggcgt tcagcatcat gacagcgtgc gtctggaccc atgggtattt atgttctcgg
     4261 caggctatcg tttctaatcg cgtgatgttg tgacgaaaaa agaccccgcc aggcggggtc
     4321 aaaaaaggac atgacacacg gattttactt gtcggaactc acttgtcttc tttcggttcg
     4381 ccgcagccta gttctgcgcc agtgagagga tcaacggtgg ggtccgattt cgttctttct
     4441 gccatcgctt tgagcagcgc catctgctcg cgcggcaatt tcactgtcgc caggccatcg
     4501 ccgccatcaa cagcaggcat cggttctgcg acatagttga agttctcatc gctattccag
     4561 ctacctcgcg gatcgtcgcc ttgcgacatg ttgtagtaca cgtcggtata ctgttctacc
     4621 ggcggtaatt tacccggcgg gaagttgtta cgaatcgaat acagcgcctt ttcaaaagag
     4681 agctggtgcg cgacttcacg ggtcattaag aaactgagtg tgtcttttac gccaggatcg
     4741 tcggtcagat taatcaggcg ttcataaatg atcttcgctc gcgcctctgc cgcgatgttt
     4801 gagcgtagat cggctgtgac ttcgccgata gtatcaatat aagccgcggt ccacggtacg
     4861 ccgccggagt ttgtcaacgc cgggccgccg ccatacagca gagaggtgat atggctatca
     4921 ttaccattct gcgtcagcga gcgataaagt tccgcttcgt tttcggtacc ttccgccagc
     4981 tcgcctttag cgcctttatt aagcattcct accagtgagc cgataatttc cagatgactc
     5041 agctcctcgg tggcgatgtc cattaacatt tctctacgac cggcatcatc atcgcttaaa
     5101 ccttgcgtga agtagcggca ggcggccgcc agttcgccct gcgggccgcc aaactgttcc
     5161 agcagcaggt tcgctaatcc aggattaggt tcgctcactc gcacagtata ttgaagttgt
     5221 tttacgtgtc gaaacatatt gcctcctgct tattatttat tttttggctt caacgccatc
     5281 tgcttcagat cgcagtaaaa attgttccgt tgtctgcgga atatgtttga taagccagtc
     5341 agccatttgc atttcttctt tcagaatggc ttcaatcgtc ggaattgagg cagtgtcgcc
     5401 agcttttttt gccgccgcca gcagggaggt ataacaagca atttcgaact gctcgaaaac
     5461 ataaccgcta attgaacctt tgacaatttc atcggaaggg aacatgccac cgatagattg
     5521 ccccattgct gccattttac tcatggagtc tttcaacacc gaacgggaaa tgccattacg
     5581 gtccaacact tcttcgagca tggtaatttg atgtttggtt tcactaatat gttgttcaat
     5641 tctggctttt atatcaggat aattttcaat acggctggcc atagattcaa gcatcgattc
     5701 tgcctgtttt tccatggcat gggcgtcacg aagccagtca tgataatgtt cagtataatt
     5761 cattttacat tcctcaatgc gtgataatct gctccgataa taaaatcgga atcatgcaag
     5821 ctgataatta aaaatattat ttcgatttgc gttcagcact tttattaaca ttgctgactg
     5881 ctaaatcggt aagttttaaa tcagtttgtt tttcctcgtc gagggtttct ttgagcagtt
     5941 ttaatgcttt gctatagccg agctgctcgg ccagggtggc tagcgtgccg tagctggcga
     6001 tttcgtaatg ctcgactttt tgcgccgcgg cgataagcgc tgcatcgcgt acttcgtttt
     6061 tctccgtact ttcgatgact tcattggctt cttcaatcag cccttccata gcgacgcatt
     6121 tcattctttt cagtttaatg ccagattcag attcgacgat ctgatcaata cgttcaatct
     6181 gaccctgggt ttcttcaaga tgagattgaa aggcctggct taatttttca ttggacgtgg
     6241 ctctggcaag tttaggaaga gccttggtta attgtttttc tgcactatag gtatctgaaa
     6301 gtagatggat aaaaaggtct tcaacggttt tgatattcat aaatctaatt ctcctgggca
     6361 aaatactgcg agccagaaga ctcgcagatt gcttgataaa agcatgtgtt atatttacat
     6421 tacagtaaat caggaattat cggatttacg tccgccgtga ctattctgac cacctttttt
     6481 accggcttca gaagctcgtt gcggatcgtt tttgaaattc ccgccgctat gctgaccgcc
     6541 tttgcggccc gcttcagatg ctttttcacg attctcggcg aaatttcctg aaccaccacg
     6601 atgttcggcc atattatttc tcctgtctcg ttgcttgctg aaaaaatcgg ttttaaccgt
     6661 gtcgacaccc tattaaaatt agttggttaa accgattagt caaattgacg gaaatagttg
     6721 tgatttaccg cgtaaataac gcacaaaaaa tggtttagag ataatatatt ctatattttc
     6781 ttttagaata agcgatttaa caggaaatgc tttgcattgt tattaataac aatcgtcact
     6841 atctgcggcc ctggaaacgt taatgcattg tttgttgaca tttctttaca taaaggcggg
     6901 ggaaattgac gtgaaaacgc taaatttttc ggcgtgatta ggagttttct tagcaaaaat
     6961 tgacttcaga ctttttgaaa gctggacggg gaataacacc aaaaagattg atgatgtacg
     7021 tctgaatcgt gattctggtt cggcgccagc cactcattaa gccgccagcg ttatgctgac
     7081 ggcttaaccc gttatgcgcg gctggcggct ttcatggctg agacaaagga cctgagctcc
     7141 gccaacatct gtttgggaga cgcgaggttt ttctcgataa tcttgacaat ggctgagccg
     7201 gagatagcgc cagccgcccc ggcacgcacg gccgcagaca cctgttccgg cgaggagata
     7261 ccgaagccct gtaacgcagg cgcggcatgg tactctttaa gcttctcaat gagatgatgc
     7321 aacggcaatg cgccacggtt ttccgcgccg gtgacacccg aacgcgaaag caggtaggtg
     7381 taaccgcggc cgtaagatgc gacctggcgc agaagatcgt catccgcatt tggcgggcag
     7441 atgaagatcg gcgcgatatt atgccgtaac gctgcctggc ggaagggggc cgattcttca
     7501 accgggacat ctgcgaccag cacggaatct acgccaacct gttcacaacg ggcatagaac
     7561 gcatctatgc cgttattgaa caccagattc gcgtacatta gcaggccaat cggaatggtc
     7621 gggtgttttt cacgaatcag cgccagcatt tcaaaacact gagccggcgt gacgccagcg
     7681 gcgaaggcgc gtaagttcgc attctggatg gtagggccat cggccagcgg atcggagaag
     7741 ggaaccccca gttccagagc gtcggcgcca gcgtcaatca gtgtgtcaat aattttcagt
     7801 gactgttcaa tgccagggtc gcccagggtc acgaagggga caaaagcgcc ttcccggcga
     7861 tcgttgagtt gggcaaataa attttcgtag cgttccatca gatttcccct cgcgctttca
     7921 ggatatcgtg tacggtaaag atgtctttat ctccgcggcc agagagattg accaccagca
     7981 gttgctcttt ttccggctgc tcgcgcatca ttttcagagc gtgcgccaac gcgtgggagg
     8041 actccagcgc cgggataatt ccctcatggc ggcacaacgt tttgaaggct tccagcgcct
     8101 catcatcggt aatggagaca taatccgcgc gtccgatgct gttcaggtac gcatgttgcg
     8161 gcccaacgga cgggaaatcg agcccggcgg aaatggaata ggactcttca atttgcccgt
     8221 ctgctgtttg catcatcggc gctttcatcc cgaaatagat gccaacgcga ccatgtttaa
     8281 gcggcgcgcc atgctcgccg gtttcaatac catgaccacc aggttcaacg cctattagcc
     8341 cgacgctggt atcattaata aaatcggcaa acatcccgat agcgtttgag ccgccaccga
     8401 cgcaagcgat aacggcatct ggcagacggc cctctttgtc gaggatttgc gctttcgtct
     8461 cttcgccaat catgcgctgg aactcgcgaa cgatggtggg atagggatgc ggtcctgccg
     8521 ccgtgccgag catatagtgc gcggtttcgt aactaccgga ccagtcgcgc agcgcctcgt
     8581 tacaggcatc ttttagcgta gcggagccgc tatgaaccgg gatgacctca gcgcccatca
     8641 gacgcatacg gaagacgttc ggcgactggc gctcaacgtc tttggcgccc atatagatac
     8701 ggcatttcag acccagcagg gcgctggcga gcgcagaggc gacgccgtgc tgaccggcgc
     8761 cggtttcagc gataatctcg cttttaccca tccgtttggc cagcagcgcc tgacccagta
     8821 cctgattggt tttgtgcgcg ccgccgtgca gtaaatcttc gcgctttaaa tacaacgtgg
     8881 tacgcgtacc ggcggtaatg ttctggcatt tcgtcagcgc ggtggggcgt cccgcgtagt
     8941 tttttagcag atcggcgaat tgcgcctgaa attcaggatc tttttgcgcg ctgacgaagg
     9001 cctcttcaag ctggttcagc gcaggcatca ggatctgcgg cacatacatg ccgccgaa
//
