Basic Vector Information
- Vector Name:
- pPIPRA561
- Antibiotic Resistance:
- Kanamycin
- Length:
- 10636 bp
- Type:
- Binary vector
- Replication origin:
- ori
- Source/Author:
- Chi-Ham CL, Boettiger S, Figueroa-Balderas R, Bird S, Geoola JN, Zamora P, Alandete-Saez M, Bennett AB.
pPIPRA561 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pPIPRA561 vector Sequence
LOCUS 40924_34679 10636 bp DNA circular SYN 18-DEC-2018
DEFINITION Binary vector pPIPRA561, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10636)
AUTHORS Chi-Ham CL, Boettiger S, Figueroa-Balderas R, Bird S, Geoola JN,
Zamora P, Alandete-Saez M, Bennett AB.
TITLE An intellectual property sharing initiative in agricultural
biotechnology: development of broadly accessible technologies for
plant transformation
JOURNAL Plant Biotechnol. J. 10 (5), 501-510 (2012)
PUBMED 22221977
REFERENCE 2 (bases 1 to 10636)
AUTHORS Chi-Ham CL, Geoola JN, Figueroa-Balderas R, Bird S, Zamora P,
Boettiger S, Bennett AB.
TITLE Direct Submission
JOURNAL Submitted (15-APR-2011) Plant Sciences, UCDavis, One Shields Avenue,
Davis, CA 95616, USA
REFERENCE 3 (bases 1 to 10636)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 10636)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Plant
Biotechnol. J."; date: "2012"; volume: "10"; issue: "5"; pages:
"501-510"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(15-APR-2011) Plant Sciences, UCDavis, One Shields Avenue, Davis, CA
95616, USA"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..10636
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 245..269
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
regulatory 350..1300
/gene="FMV34S"
/regulatory_class="promoter"
CDS 1318..2106
/label=NeoR/KanR
/note="aminoglycoside phosphotransferase"
terminator 2127..2379
/label=MAS terminator
/note="mannopine synthase terminator"
regulatory 2430..3380
/gene="FMV34S"
/regulatory_class="promoter"
misc_feature 3391..3445
/label=MCS (polylinker)
/note="MCS (polylinker)"
regulatory 3451..3755
/label=pea E9 3' UTR
/note="pea E9 3' UTR"
/regulatory_class="terminator"
misc_feature 3844..3868
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
CDS 5320..5946
/label=pVS1 StaA
/note="stability protein from the Pseudomonas plasmid pVS1
(Heeb et al., 2000)"
CDS 6378..7448
/label=pVS1 RepA
/note="replication protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
rep_origin 7517..7711
/label=pVS1 oriV
/note="origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
misc_feature 8185..8325
/label=bom
/note="basis of mobility region from pBR322"
rep_origin complement(8511..9099)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(9567..10358)
/label=KanR
/note="aminoglycoside phosphotransferase"
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