LOCUS       40924_34679       10636 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pPIPRA561, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10636)
  AUTHORS   Chi-Ham CL, Boettiger S, Figueroa-Balderas R, Bird S, Geoola JN, 
            Zamora P, Alandete-Saez M, Bennett AB.
  TITLE     An intellectual property sharing initiative in agricultural 
            biotechnology: development of broadly accessible technologies for 
            plant transformation
  JOURNAL   Plant Biotechnol. J. 10 (5), 501-510 (2012)
  PUBMED    22221977
REFERENCE   2  (bases 1 to 10636)
  AUTHORS   Chi-Ham CL, Geoola JN, Figueroa-Balderas R, Bird S, Zamora P, 
            Boettiger S, Bennett AB.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2011) Plant Sciences, UCDavis, One Shields Avenue,
            Davis, CA 95616, USA
REFERENCE   3  (bases 1 to 10636)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10636)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Biotechnol. J."; date: "2012"; volume: "10"; issue: "5"; pages: 
            "501-510"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (15-APR-2011) Plant Sciences, UCDavis, One Shields Avenue, Davis, CA
            95616, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10636
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    245..269
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     regulatory      350..1300
                     /gene="FMV34S"
                     /regulatory_class="promoter"
     CDS             1318..2106
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     terminator      2127..2379
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     regulatory      2430..3380
                     /gene="FMV34S"
                     /regulatory_class="promoter"
     misc_feature    3391..3445
                     /label=MCS (polylinker)
                     /note="MCS (polylinker)"
     regulatory      3451..3755
                     /label=pea E9 3' UTR
                     /note="pea E9 3' UTR"
                     /regulatory_class="terminator"
     misc_feature    3844..3868
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             5320..5946
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             6378..7448
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      7517..7711
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    8185..8325
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8511..9099)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9567..10358)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 tctagatgta cacaggcagc aacgctctgt catcgttaca atcaacatgc taccctccgc
       61 gagatcatcc gtgtttcaaa cccggcagct tagttgccgt tcttccgaat agcatcggta
      121 acatgagcaa agtctgccgc cttacaacgg ctctcccgct gacgccgtcc cggactgatg
      181 ggctgcctgt atcgagtggt gattttgtgc cgagctgccg gtcggggagc tgttggctgg
      241 ctggtggcag gatatattgt ggtgtaaaca aattgacgct tagacaactt aataacacat
      301 tgcggacgtt tttaatgtac tgaattaacg ccgaatttgt acaccgcgga tcgatgaatt
      361 cagacaaatt tgggaaaaat ccagattatc tgatcttgaa gatggagtcg ctgaaaaatt
      421 ctacacagaa gataaagcta caaaatccct cttcacattc acagaaaatg cagaaccata
      481 tcttgttcat gcagctttcc gtgcaggatt agcaaaggta atctacccta gtccaaatct
      541 ccaagaactc aaatggttcc caagagatat cgttaaggca atcaaacatt tccggaagaa
      601 agtgttaaac gccaaagact cagcaatcta catcaagatt ttcagcagtc taccagattg
      661 gaaacaatca gaaaggttca cgccttacca tttcttgcaa attggtattt ccaaagcaaa
      721 gaaagagctc ccaagcacga aggcatgtgt cggaaaagat tttccaaccg agcagcagat
      781 ggcagcaatc agatcatcaa gtctgaaaac catagtcgaa aaattacagg agataaaaaa
      841 tgactcaact gtaaaaatca actatgactc aagcacatgc atcatctcga gcaagtgtac
      901 aaaaaagatt accacggaag aaattcacaa ggtaaaactt tttgagaaaa gattgatcaa
      961 catcaatcaa atcaattgtg gagaccacac aaagaaattg tggtgtaaaa ttctacagaa
     1021 aaagtacgaa gagcatcttt gccagtactg cagtggggac cacccacgtg acaacaaaga
     1081 agtggcagac aagtcacctc aaagtggaac ccatccacgt gataaaagta tcatggcaga
     1141 cagccggtac aataatgggg aggacagctt gcaaagcagc ccatgtggaa gcccactcac
     1201 aaacgcgtat tacgaacgca gtgacgacat ccactcaaga attccatcta tttaaagacg
     1261 gattcattcc catttgaaga tcatcaatac tcaaccaata tcgataccgg tatggggatt
     1321 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
     1381 gactgggcac aacagacaat cggctgctct gatgccgccg tgtttcggct gtcagcgcag
     1441 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actccaggac
     1501 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac
     1561 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
     1621 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
     1681 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
     1741 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat
     1801 caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag
     1861 gatctcgtcg tgacacatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
     1921 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg
     1981 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg
     2041 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag
     2101 ttcttctgaa ccggtgcgat cgcaatcttg gactcccatg ttggcaaagg caaccaaaca
     2161 aacaatgaat gatccgctcc tgcatatggg gcggtttgag tatttcaact gccatttggg
     2221 ctgaattgaa gacatgctcc tgtcagaaat tccgtgatct tactcaatat tcagtaatct
     2281 cggccaatat cctaaatgtg cgtggcttta tctgtctttg tattgtttca tcaattcatg
     2341 taacgtttgc ttttcttatg aattttcaaa taaattatca gatcgcgatc gcccgcggtg
     2401 actgactgac ttaattaatg catggcgcca tcgatgaatt cagacaaatt tgggaaaaat
     2461 ccagattatc tgatcttgaa gatggagtcg ctgaaaaatt ctacacagaa gataaagcta
     2521 caaaatccct cttcacattc acagaaaatg cagaaccata tcttgttcat gcagctttcc
     2581 gtgcaggatt agcaaaggta atctacccta gtccaaatct ccaagaactc aaatggttcc
     2641 caagagatat cgttaaggca atcaaacatt tccggaagaa agtgttaaac gccaaagact
     2701 cagcaatcta catcaagatt ttcagcagtc taccagattg gaaacaatca gaaaggttca
     2761 cgccttacca tttcttgcaa attggtattt ccaaagcaaa gaaagagctc ccaagcacga
     2821 aggcatgtgt cggaaaagat tttccaaccg agcagcagat ggcagcaatc agatcatcaa
     2881 gtctgaaaac catagtcgaa aaattacagg agataaaaaa tgactcaact gtaaaaatca
     2941 actatgactc aagcacatgc atcatctcga gcaagtgtac aaaaaagatt accacggaag
     3001 aaattcacaa ggtaaaactt tttgagaaaa gattgatcaa catcaatcaa atcaattgtg
     3061 gagaccacac aaagaaattg tggtgtaaaa ttctacagaa aaagtacgaa gagcatcttt
     3121 gccagtactg cagtggggac cacccacgtg acaacaaaga agtggcagac aagtcacctc
     3181 aaagtggaac ccatccacgt gataaaagta tcatggcaga cagccggtac aataatgggg
     3241 aggacagctt gcaaagcagc ccatgtggaa gcccactcac aaacgcgtat tacgaacgca
     3301 gtgacgacat ccactcaaga attccatcta tttaaagacg gattcattcc catttgaaga
     3361 tcatcaatac tcaaccaata tcgatgatat ccaaagcttt ggcggccgcc tgcaggtcga
     3421 cgagctcgag aattcggatc cccgggtacc gcgatcgctt cgagtattat ggcattggga
     3481 aaactgtttt tcttgtacca tttgttgtgc ttgtaattta ctgtgttttt tattcggttt
     3541 tcgctatcga actgtgaaat ggaaatggat ggagaagagt taatgaatga tatggtcctt
     3601 ttgttcattc tcaaattaat attatttgtt ttttctctta tttgttgtgt gttgaatttg
     3661 aaattataag agatatgcaa acattttgtt ttgagtaaaa atgtgtcaaa tcgtggcctc
     3721 taatgaccga agttaatatg aggagtaaaa catcgcgatc gcggcgccat gcattaatta
     3781 acaattgagc ttgagcttgg atcagattgt cgtttcccgc cttcagttta aactatcagt
     3841 gtttgacagg atatattggc gggtaaacct aagagaaaag agcgtttatt agaataacgg
     3901 atatttaaaa gggcgtgaaa aggtttatcc gttcgtccat ttgtatgtgc atgccaacca
     3961 cagggttccc ctcgggatca acaattgtct agacatgaca ttaacctata aaaataggcg
     4021 tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgagcgct aaaacctctg
     4081 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagata
     4141 gggcccaacc cctccgctgc tatagtgcag tcggcttctg acgttcagtg cagccgtctt
     4201 ctgaaaacga catgtcgcac aagtcctaag ttacgcgaca ggctgccgcc ctgccctttt
     4261 cctggcgttt tcttgtcgcg tgttttagtc gcataaagta gaatacttgc gactagaacc
     4321 ggagacatta cgccatgaac aagagcgccg ccgctggcct gctgggctat gcccgcgtca
     4381 gcaccgacga ccaggacttg accaaccaac gggccgaact gcacgcggcc ggctgcacca
     4441 agctgttttc cgagaagatc accggcacca ggcgcgaccg cccggagctg gccaggatgc
     4501 ttgaccacct acgccctggc gacgttgtga cagtgaccag gctagaccgc ctggcccgca
     4561 gcacccgcga cctactggac attgccgagc gcatccagga ggccggcgcg ggcctgcgta
     4621 gcctggcaga gccgtgggcc gacaccacca cgccggccgg ccgcatggtg ttgaccgtgt
     4681 tcgccggcat tgccgagttc gagcgttccc taatcatcga ccgcacccgg agcgggcgcg
     4741 aggccgccaa ggcccgaggc gtgaagtttg gcccccgccc taccctcacc ccggcacaga
     4801 tcgcgcacgc ccgcgagctg atcgaccagg aaggccgcac cgtgaaagag gcggctgcac
     4861 tgcttggcgt gcatcgctcg accctgtacc gcgcacttga gcgcagcgag gaagtgacgc
     4921 ccaccgaggc caggcggcgc ggtgccttcc gtgaggacgc attgaccgag gccgacgccc
     4981 tggcggccgc cgagaatgaa cgccaagagg aacaagcatg aaaccgcacc aggacggcca
     5041 ggacgaaccg tttttcatta ccgaagagat cgaggcggag atgatcgcgg ccgggtacgt
     5101 gttcgagccg cccgcgcacg tctcaaccgt gcggctgcat gaaatcctgg ccggtttgtc
     5161 tgatgccaag ctggcggcct ggccggccag cttggccgct gaagaaaccg agcgccgccg
     5221 tctaaaaagg tgatgtgtat ttgagtaaaa cagcttgcgt catgcggtcg ctgcgtatat
     5281 gatgcgatga gtaaataaac aaatacgcaa ggggaacgca tgaaggttat cgctgtactt
     5341 aaccagaaag gcgggtcagg caagacgacc atcgcaaccc atctagcccg cgccctgcaa
     5401 ctcgccgggg ccgatgttct gttagtcgat tccgatcccc agggcagtgc ccgcgattgg
     5461 gcggccgtgc gggaagatca accgctaacc gttgtcggca tcgaccgccc gacgattgac
     5521 cgcgacgtga aggccatcgg ccggcgcgac ttcgtagtga tcgacggagc gccccaggcg
     5581 gcggacttgg ctgtgtccgc gatcaaggca gccgacttcg tgctgattcc ggtgcagcca
     5641 agcccttacg acatatgggc caccgccgac ctggtggagc tggttaagca gcgcattgag
     5701 gtcacggatg gaaggctaca agcggccttt gtcgtgtcgc gggcgatcaa aggcacgcgc
     5761 atcggcggtg aggttgccga ggcgctggcc gggtacgagc tgcccattct tgagtcccgt
     5821 atcacgcagc gcgtgagcta cccaggcact gccgccgccg gcacaaccgt tcttgaatca
     5881 gaacccgagg gcgacgctgc ccgcgaggtc caggcgctgg ccgctgaaat taaatcaaaa
     5941 ctcatttgag ttaatgaggt aaagagaaaa tgagcaaaag cacaaacacg ctaagtgccg
     6001 gccgtccgag cgcacgcagc agcaaggctg caacgttggc cagcctggca gacacgccag
     6061 ccatgaagcg ggtcaacttt cagttgccgg cggaggatca caccaagctg aagatgtacg
     6121 cggtacgcca aggcaagacc attaccgagc tgctatctga atacatcgcg cagctaccag
     6181 agtaaatgag caaatgaata aatgagtaga tgaattttag cggctaaagg aggcggcatg
     6241 gaaaatcaag aacaaccagg caccgacgcc gtggaatgcc ccatgtgtgg aggaacgggc
     6301 ggttggccag gcgtaagcgg ctgggttgtc tgccggccct gcaatggcac tggaaccccc
     6361 aagcccgagg aatcggcgtg agcggtcgca aaccatccgg cccggtacaa atcggcgcgg
     6421 cgctgggtga tgacctggtg gagaagttga aggccgcgca ggccgcccag cggcaacgca
     6481 tcgaggcaga agcacgcccc ggtgaatcgt ggcaagcggc cgctgatcga atccgcaaag
     6541 aatcccggca accgccggca gccggtgcgc cgtcgattag gaagccgccc aagggcgacg
     6601 agcaaccaga ttttttcgtt ccgatgctct atgacgtggg cacccgcgat agtcgcagca
     6661 tcatggacgt ggccgttttc cgtctgtcga agcgtgaccg acgagctggc gaggtgatcc
     6721 gctacgagct tccagacggg cacgtagagg tttccgcagg gccggccggc atggccagtg
     6781 tgtgggatta cgacctggta ctgatggcgg tttcccatct aaccgaatcc atgaaccgat
     6841 accgggaagg gaagggagac aagcccggcc gcgtgttccg tccacacgtt gcggacgtac
     6901 tcaagttctg ccggcgagcc gatggcggaa agcagaaaga cgacctggta gaaacctgca
     6961 ttcggttaaa caccacgcac gttgccatgc agcgtacgaa gaaggccaag aacggccgcc
     7021 tggtgacggt atccgagggt gaagccttga ttagccgcta caagatcgta aagagcgaaa
     7081 ccgggcggcc ggagtacatc gagatcgagc tagctgattg gatgtaccgc gagatcacag
     7141 aaggcaagaa cccggacgtg ctgacggttc accccgatta ctttttgatc gatcccggca
     7201 tcggccgttt tctctaccgc ctggcacgcc gcgccgcagg caaggcagaa gccagatggt
     7261 tgttcaagac gatctacgaa cgcagtggca gcgccggaga gttcaagaag ttctgtttca
     7321 ccgtgcgcaa gctgatcggg tcaaatgacc tgccggagta cgatttgaag gaggaggcgg
     7381 ggcaggctgg cccgatccta gtcatgcgct accgcaacct gatcgagggc gaagcatccg
     7441 ccggttccta atgtacggag cagatgctag ggcaaattgc cctagcaggg gaaaaaggtc
     7501 gaaaaggtct ctttcctgtg gatagcacgt acattgggaa cccaaagccg tacattggga
     7561 accggaaccc gtacattggg aacccaaagc cgtacattgg gaaccggtca cacatgtaag
     7621 tgactgatat aaaagagaaa aaaggcgatt tttccgccta aaactcttta aaacttatta
     7681 aaactcttaa aacccgcctg gcctgtgcat aactgtctgg ccagcgcaca gccgaagagc
     7741 tgcaaaaagc gcctaccctt cggtcgctgc gctccctacg ccccgccgct tcgcgtcggc
     7801 ctatcgcggc cgctggccgc tcaaaaatgg ctggcctacg gccaggcaat ctaccagggc
     7861 gcggacaagc cgcgccgtcg ccactcgacc gccggcgctg ggcccttatt gaagcattta
     7921 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa accatggtaa
     7981 acaaataggg gttccgcgca catttccccg aaaagtgcca ccttaaacca ttattatgac
     8041 gtcctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
     8101 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
     8161 agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt
     8221 gtatactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg
     8281 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc
     8341 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     8401 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     8461 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     8521 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     8581 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     8641 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     8701 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     8761 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     8821 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     8881 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     8941 tacactagaa ggacagtatt tggtatctcc gctctgctga agccagttac cttcggaaaa
     9001 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     9061 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     9121 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catggacgtc
     9181 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     9241 ctcaaaggcg gtaggcgcgc catcgatggc acgaacccag tggacataag cctcgttcgg
     9301 ttcgtaagct gtaatgcaag tagcgtaact gccgtcacgc aactggtcca gaaccttgac
     9361 cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg gcttcttgtt atgacatgtt
     9421 tttttggggt acagtctatg cctcgggcat ccaagcagca agcgcgttac gccgtgggtc
     9481 gatgtttgat gttatggagc agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa
     9541 agttaaacat catcgatacc ggtctaaaac aattcatcca gtaaaatata atattttatt
     9601 ttctcccaat caggcttgat ccccagtaag tcaaaaaata gctcgacata ctgttcttcc
     9661 ccgatatcct ccctgatcga ccggacgcag aaggcaatgt cataccactt gtccgccctg
     9721 ccgcttctcc caagatcaat aaagccactt actttgccat ctttcacaaa gatgttgctg
     9781 tctcccaggt cgccgtggga aaagacaagt tcctcttcgg gcttttccgt ctttaaaaaa
     9841 tcatacagct cgcgcggatc tttaaatgga gtgtcttctt cccagttttc gcaatccaca
     9901 tcggccagat cgttattcag taagtaatcc aattcggcta agcggctgtc taagctattc
     9961 gtatagggac aatccgatat gtcgatggag tgaaagagcc tgatgcactc cgcatacagc
    10021 tcgataatct tttcagggct ttgttcatct tcatactctt ccgagcaaag gacgccatcg
    10081 gcctcactca tgagcagatt gctccagcca tcatgccgtt caaagtgcag gacctttgga
    10141 acaggcagct ttccttccag ccatagcatc atgtcctttt cccgttccac atcataggtg
    10201 gtccctttat accggctgtc cgtcattttt aaatataggt tttcattttc tcccaccagc
    10261 ttatatacct tagcaggaga cattccttcc gtatctttta cgcagcggta tttttcgatc
    10321 agttttttca attccggtga tattctcatt ttagccatac cggtgcgatc gctgtctaac
    10381 aattcgttca agccgacgcc gcttcgccgg cgttaactca agcgattaga tgcactaagc
    10441 acataattgc tcacagccaa actatcaggt caagtctgct tttattattt ttaagcgtgc
    10501 ataataagcc ctacacaaat tgggagatat atcatggcga tcgcggcgcg cctgactgac
    10561 tgacatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
    10621 ccagcaaaag gccagg
//
