Basic Vector Information
- Vector Name:
- pPIPRA522
- Antibiotic Resistance:
- Ampicillin
- Length:
- 4012 bp
- Type:
- Shuttle vector
- Replication origin:
- ori
- Source/Author:
- Chi-Ham CL, Boettiger S, Figueroa-Balderas R, Bird S, Geoola JN, Zamora P, Alandete-Saez M, Bennett AB.
pPIPRA522 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pPIPRA522 vector Sequence
LOCUS 40924_34659 4012 bp DNA circular SYN 18-DEC-2018
DEFINITION Shuttle vector pPIPRA522, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4012)
AUTHORS Chi-Ham CL, Boettiger S, Figueroa-Balderas R, Bird S, Geoola JN,
Zamora P, Alandete-Saez M, Bennett AB.
TITLE An intellectual property sharing initiative in agricultural
biotechnology: development of broadly accessible technologies for
plant transformation
JOURNAL Plant Biotechnol. J. 10 (5), 501-510 (2012)
PUBMED 22221977
REFERENCE 2 (bases 1 to 4012)
AUTHORS Chi-Ham CL, Geoola JN, Figueroa-Balderas R, Bird S, Zamora P,
Boettiger S, Bennett AB.
TITLE Direct Submission
JOURNAL Submitted (15-APR-2011) Plant Sciences, UCDavis, One Shields Avenue,
Davis, CA 95616, USA
REFERENCE 3 (bases 1 to 4012)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 4012)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Plant
Biotechnol. J."; date: "2012"; volume: "10"; issue: "5"; pages:
"501-510"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(15-APR-2011) Plant Sciences, UCDavis, One Shields Avenue, Davis, CA
95616, USA"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..4012
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 379..395
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
regulatory 421..1371
/gene="FMV34S"
/regulatory_class="promoter"
gene 421..1371
/gene="FMV34S"
/label=FMV34S
misc_feature 1386..1440
/label=polylinker
/note="polylinker"
regulatory 1442..1746
/label=Pea E9 3' UTR
/note="Pea E9 3' UTR"
/regulatory_class="terminator"
primer_bind complement(1791..1807)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(1815..1831)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(1839..1869)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(1884..1905)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(2193..2781)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(2955..3812)
/label=AmpR
/note="beta-lactamase"
promoter complement(3813..3917)
/label=AmpR promoter
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