pPIPRA522 vector (V004094)

LOCUS       40924_34659        4012 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Shuttle vector pPIPRA522, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4012)
  AUTHORS   Chi-Ham CL, Boettiger S, Figueroa-Balderas R, Bird S, Geoola JN, 
            Zamora P, Alandete-Saez M, Bennett AB.
  TITLE     An intellectual property sharing initiative in agricultural 
            biotechnology: development of broadly accessible technologies for 
            plant transformation
  JOURNAL   Plant Biotechnol. J. 10 (5), 501-510 (2012)
  PUBMED    22221977
REFERENCE   2  (bases 1 to 4012)
  AUTHORS   Chi-Ham CL, Geoola JN, Figueroa-Balderas R, Bird S, Zamora P, 
            Boettiger S, Bennett AB.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2011) Plant Sciences, UCDavis, One Shields Avenue,
            Davis, CA 95616, USA
REFERENCE   3  (bases 1 to 4012)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4012)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Biotechnol. J."; date: "2012"; volume: "10"; issue: "5"; pages: 
            "501-510"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (15-APR-2011) Plant Sciences, UCDavis, One Shields Avenue, Davis, CA
            95616, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4012
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      421..1371
                     /gene="FMV34S"
                     /regulatory_class="promoter"
     gene            421..1371
                     /gene="FMV34S"
                     /label=FMV34S
     misc_feature    1386..1440
                     /label=polylinker
                     /note="polylinker"
     regulatory      1442..1746
                     /label=Pea E9 3' UTR
                     /note="Pea E9 3' UTR"
                     /regulatory_class="terminator"
     primer_bind     complement(1791..1807)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1815..1831)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1839..1869)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1884..1905)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2193..2781)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2955..3812)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3813..3917)
                     /label=AmpR promoter