LOCUS       40924_34659        4012 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Shuttle vector pPIPRA522, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4012)
  AUTHORS   Chi-Ham CL, Boettiger S, Figueroa-Balderas R, Bird S, Geoola JN, 
            Zamora P, Alandete-Saez M, Bennett AB.
  TITLE     An intellectual property sharing initiative in agricultural 
            biotechnology: development of broadly accessible technologies for 
            plant transformation
  JOURNAL   Plant Biotechnol. J. 10 (5), 501-510 (2012)
  PUBMED    22221977
REFERENCE   2  (bases 1 to 4012)
  AUTHORS   Chi-Ham CL, Geoola JN, Figueroa-Balderas R, Bird S, Zamora P, 
            Boettiger S, Bennett AB.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2011) Plant Sciences, UCDavis, One Shields Avenue,
            Davis, CA 95616, USA
REFERENCE   3  (bases 1 to 4012)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4012)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Biotechnol. J."; date: "2012"; volume: "10"; issue: "5"; pages: 
            "501-510"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (15-APR-2011) Plant Sciences, UCDavis, One Shields Avenue, Davis, CA
            95616, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4012
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      421..1371
                     /gene="FMV34S"
                     /regulatory_class="promoter"
     gene            421..1371
                     /gene="FMV34S"
                     /label=FMV34S
     misc_feature    1386..1440
                     /label=polylinker
                     /note="polylinker"
     regulatory      1442..1746
                     /label=Pea E9 3' UTR
                     /note="Pea E9 3' UTR"
                     /regulatory_class="terminator"
     primer_bind     complement(1791..1807)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1815..1831)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1839..1869)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1884..1905)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2193..2781)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2955..3812)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3813..3917)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gttaattaat gcatggcgcc
      421 atcgatgaat tcagacaaat ttgggaaaaa tccagattat ctgatcttga agatggagtc
      481 gctgaaaaat tctacacaga agataaagct acaaaatccc tcttcacatt cacagaaaat
      541 gcagaaccat atcttgttca tgcagctttc cgtgcaggat tagcaaaggt aatctaccct
      601 agtccaaatc tccaagaact caaatggttc ccaagagata tcgttaaggc aatcaaacat
      661 ttccggaaga aagtgttaaa cgccaaagac tcagcaatct acatcaagat tttcagcagt
      721 ctaccagatt ggaaacaatc agaaaggttc acgccttacc atttcttgca aattggtatt
      781 tccaaagcaa agaaagagct cccaagcacg aaggcatgtg tcggaaaaga ttttccaacc
      841 gagcagcaga tggcagcaat cagatcatca agtctgaaaa ccatagtcga aaaattacag
      901 gagataaaaa atgactcaac tgtaaaaatc aactatgact caagcacatg catcatctcg
      961 agcaagtgta caaaaaagat taccacggaa gaaattcaca aggtaaaact ttttgagaaa
     1021 agattgatca acatcaatca aatcaattgt ggagaccaca caaagaaatt gtggtgtaaa
     1081 attctacaga aaaagtacga agagcatctt tgccagtact gcagtgggga ccacccacgt
     1141 gacaacaaag aagtggcaga caagtcacct caaagtggaa cccatccacg tgataaaagt
     1201 atcatggcag acagccggta caataatggg gaggacagct tgcaaagcag cccatgtgga
     1261 agcccactca caaacgcgta ttacgaacgc agtgacgaca tccactcaag aattccatct
     1321 atttaaagac ggattcattc ccatttgaag atcatcaata ctcaaccaat atcgatgata
     1381 tccaaagctt tggcggccgc ctgcaggtcg acgagctcga gaattcggat ccccgggtac
     1441 cgcgatcgct tcgagtatta tggcattggg aaaactgttt ttcttgtacc atttgttgtg
     1501 cttgtaattt actgtgtttt ttattcggtt ttcgctatcg aactgtgaaa tggaaatgga
     1561 tggagaagag ttaatgaatg atatggtcct tttgttcatt ctcaaattaa tattatttgt
     1621 tttttctctt atttgttgtg tgttgaattt gaaattataa gagatatgca aacattttgt
     1681 tttgagtaaa aatgtgtcaa atcgtggcct ctaatgaccg aagttaatat gaggagtaaa
     1741 acatcgcgat cgcggcgcca tgcattaatt aagagcttgg cgtaatcatg gtcatagctg
     1801 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     1861 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     1921 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     1981 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     2041 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     2101 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     2161 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     2221 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     2281 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     2341 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     2401 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     2461 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     2521 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     2581 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     2641 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     2701 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     2761 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     2821 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     2881 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     2941 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     3001 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     3061 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     3121 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     3181 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     3241 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     3301 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     3361 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     3421 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     3481 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     3541 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     3601 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     3661 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     3721 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     3781 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     3841 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     3901 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
     3961 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tc
//
