pPICZBBAX vector (V004101)

MSDS

Basic Vector Information

Vector Name:: pPICZBBAX

Antibiotic Resistance:: Bleomycin

Length:: 3912 bp

Type:: Yeast expression vector

Replication origin:: ori

Source/Author:: De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman Fonseca M, Vanhoucke M, Beyaert R.

Promoter:: AOX1

pPICZBBAX vector Vector Map

View Map in NovoBuilder

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pPICZBBAX vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       V004101                 3912 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004101
VERSION     V004101
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3912)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3912)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 3912)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3912)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3912
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             17..46
                     /label="Myc"
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     CDS             62..79
                     /label="6xHis"
                     /note="6xHis affinity tag"
     terminator      159..405
                     /label="AOX1 terminator"
                     /note="transcription terminator for AOX1"
     promoter        420..831
                     /label="TEF1 promoter"
                     /note="promoter for EF-1-alpha"
     promoter        839..886
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     CDS             905..1276
                     /label="BleoR"
                     /note="antibiotic-binding protein"
     terminator      1345..1592
                     /label="CYC1 terminator"
                     /note="transcription terminator for CYC1"
     rep_origin      complement(1667..2255)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     promoter        2337..3275
                     /label="AOX1 promoter"
                     /note="inducible promoter, regulated by methanol"
     CDS             3314..3889
                     /gene="Bax"
                     /label="Apoptosis regulator BAX"
                     /note="Apoptosis regulator BAX from Mus musculus.
                     Accession#: Q07813"

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