LOCUS       V004101                 3912 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004101
VERSION     V004101
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3912)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3912)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 3912)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3912)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3912
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             17..46
                     /label="Myc"
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     CDS             62..79
                     /label="6xHis"
                     /note="6xHis affinity tag"
     terminator      159..405
                     /label="AOX1 terminator"
                     /note="transcription terminator for AOX1"
     promoter        420..831
                     /label="TEF1 promoter"
                     /note="promoter for EF-1-alpha"
     promoter        839..886
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     CDS             905..1276
                     /label="BleoR"
                     /note="antibiotic-binding protein"
     terminator      1345..1592
                     /label="CYC1 terminator"
                     /note="transcription terminator for CYC1"
     rep_origin      complement(1667..2255)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     promoter        2337..3275
                     /label="AOX1 promoter"
                     /note="inducible promoter, regulated by methanol"
     CDS             3314..3889
                     /gene="Bax"
                     /label="Apoptosis regulator BAX"
                     /note="Apoptosis regulator BAX from Mus musculus.
                     Accession#: Q07813"
ORIGIN
        1 ggccgccagc tttctagaac aaaaactcat ctcagaagag gatctgaata gcgccgtcga
       61 ccatcatcat catcatcatt gagtttgtag ccttagacat gactgttcct cagttcaagt
      121 tgggcactta cgagaagacc ggtcttgcta gattctaatc aagaggatgt cagaatgcca
      181 tttgcctgag agatgcaggc ttcatttttg atactttttt atttgtaacc tatatagtat
      241 aggatttttt ttgtcatttt gtttcttctc gtacgagctt gctcctgatc agcctatctc
      301 gcagctgatg aatatcttgt ggtaggggtt tgggaaaatc attcgagttt gatgtttttc
      361 ttggtatttc ccactcctct tcagagtaca gaagattaag tgagaccttc gtttgtgcgg
      421 atcccccaca caccatagct tcaaaatgtt tctactcctt ttttactctt ccagattttc
      481 tcggactccg cgcatcgccg taccacttca aaacacccaa gcacagcata ctaaattttc
      541 cctctttctt cctctagggt gtcgttaatt acccgtacta aaggtttgga aaagaaaaaa
      601 gagaccgcct cgtttctttt tcttcgtcga aaaaggcaat aaaaattttt atcacgtttc
      661 tttttcttga aatttttttt tttagttttt ttctctttca gtgacctcca ttgatattta
      721 agttaataaa cggtcttcaa tttctcaagt ttcagtttca tttttcttgt tctattacaa
      781 ctttttttac ttcttgttca ttagaaagaa agcatagcaa tctaatctaa ggggcggtgt
      841 tgacaattaa tcatcggcat agtatatcgg catagtataa tacgacaagg tgaggaacta
      901 aaccatggcc aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg tcgccggagc
      961 ggtcgagttc tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc
     1021 cggtgtggtc cgggacgacg tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc
     1081 ggacaacacc ctggcctggg tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc
     1141 ggaggtcgtg tccacgaact tccgggacgc ctccgggccg gccatgaccg agatcggcga
     1201 gcagccgtgg gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg tgcacttcgt
     1261 ggccgaggag caggactgac acgtccgacg gcggcccacg ggtcccaggc ctcggagatc
     1321 cgtccccctt ttcctttgtc gatatcatgt aattagttat gtcacgctta cattcacgcc
     1381 ctccccccac atccgctcta accgaaaagg aaggagttag acaacctgaa gtctaggtcc
     1441 ctatttattt ttttatagtt atgttagtat taagaacgtt atttatattt caaatttttc
     1501 ttttttttct gtacagacgc gtgtacgcat gtaacattat actgaaaacc ttgcttgaga
     1561 aggttttggg acgctcgaag gctttaattt gcaagctgga gaccaacatg tgagcaaaag
     1621 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     1681 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     1741 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     1801 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     1861 aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     1921 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     1981 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     2041 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     2101 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     2161 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     2221 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     2281 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agatcagatc
     2341 taacatccaa agacgaaagg ttgaatgaaa cctttttgcc atccgacatc cacaggtcca
     2401 ttctcacaca taagtgccaa acgcaacagg aggggataca ctagcagcag accgttgcaa
     2461 acgcaggacc tccactcctc ttctcctcaa cacccacttt tgccatcgaa aaaccagccc
     2521 agttattggg cttgattgga gctcgctcat tccaattcct tctattaggc tactaacacc
     2581 atgactttat tagcctgtct atcctggccc ccctggcgag gttcatgttt gtttatttcc
     2641 gaatgcaaca agctccgcat tacacccgaa catcactcca gatgagggct ttctgagtgt
     2701 ggggtcaaat agtttcatgt tccccaaatg gcccaaaact gacagtttaa acgctgtctt
     2761 ggaacctaat atgacaaaag cgtgatctca tccaagatga actaagtttg gttcgttgaa
     2821 atgctaacgg ccagttggtc aaaaagaaac ttccaaaagt cggcataccg tttgtcttgt
     2881 ttggtattga ttgacgaatg ctcaaaaata atctcattaa tgcttagcgc agtctctcta
     2941 tcgcttctga accccggtgc acctgtgccg aaacgcaaat ggggaaacac ccgctttttg
     3001 gatgattatg cattgtctcc acattgtatg cttccaagat tctggtggga atactgctga
     3061 tagcctaacg ttcatgatca aaatttaact gttctaaccc ctacttgaca gcaatatata
     3121 aacagaagga agctgccctg tcttaaacct ttttttttat catcattatt agcttacttt
     3181 cataattgcg actggttcca attgacaagc ttttgatttt aacgactttt aacgacaact
     3241 tgagaagatc aaaaaacaac taattattcg aaacgaggaa ttcgagctcg gtacccgggg
     3301 atcctctaga gtcatggacg ggtccgggga gcagcttggg agcggcgggc ccaccagctc
     3361 tgaacagatc atgaagacag gggccttttt gctacagggt ttcatccagg atcgagcagg
     3421 gaggatggct ggggagacac ctgagctgac cttggagcag ccgccccagg atgcgtccac
     3481 caagaagctg agcgagtgtc tccggcgaat tggagatgaa ctggatagca atatggagct
     3541 gcagaggatg attgctgacg tggacacgga ctccccccga gaggtcttct tccgggtggc
     3601 agctgacatg tttgctgatg gcaacttcaa ctggggccgc gtggttgccc tcttctactt
     3661 tgctagcaaa ctggtgctca aggccctgtg cactaaagtg cccgagctga tcagaaccat
     3721 catgggctgg acactggact tcctccgtga gcggctgctt gtctggatcc aagaccaggg
     3781 tggctgggaa ggcctcctct cctacttcgg gacccccaca tggcagacag tgaccatctt
     3841 tgtggctgga gtcctcaccg cctcgctcac catctggaag aagatgggct gagacctgca
     3901 ggcatgcaag ct
//
