Basic Vector Information
- Vector Name:
- pPIBTcosTET
- Antibiotic Resistance:
- Ampicillin
- Length:
- 7384 bp
- Type:
- Cosmid vector
- Replication origin:
- ori
- Source/Author:
- Oertel W, Weissgerber A.
- Promoter:
- tet
pPIBTcosTET vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pPIBTcosTET vector Sequence
LOCUS 40924_34490 7384 bp DNA circular SYN 18-DEC-2018
DEFINITION Cosmid vector pPIBTcosTET, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7384)
AUTHORS Oertel W, Weissgerber A.
TITLE Transformation of Ulva mutabilis Foyn with plasmids integrating into
the chromosome
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 7384)
AUTHORS Oertel W, Weissgerber A.
TITLE Direct Submission
JOURNAL Submitted (19-OCT-2007) Biochemistry, Genetics and Microbiology,
Division of Genetics, University of Regensburg, Universitaetsstr.
31, Regensburg, Bavaria 93053, Germany
REFERENCE 3 (bases 1 to 7384)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 7384)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(19-OCT-2007) Biochemistry, Genetics and Microbiology, Division of
Genetics, University of Regensburg, Universitaetsstr. 31,
Regensburg, Bavaria 93053, Germany"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..7384
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 1..546
/label=contains Ulva mutabilis RbcS promoter and 5' UTR
/note="contains Ulva mutabilis RbcS promoter and 5' UTR"
intron 555..1051
/gene="ble"
/note="Ulva mutabilis RbcS intron 1"
CDS 1050..1415
/label=BleoR
/note="antibiotic-binding protein"
3'UTR 1419..1807
/label=Ulva mutabilis RbcS 3' UTR
/note="Ulva mutabilis RbcS 3' UTR"
misc_feature 1816..1986
/label=cloning vector pUC18, with minor modifications
/note="cloning vector pUC18, with minor modifications"
CDS complement(3585..4772)
/label=TcR
/note="tetracycline efflux protein"
promoter complement(4820..4848)
/label=tet promoter
/note="E. coli promoter for tetracycline efflux protein
gene"
primer_bind 5249..5265
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
misc_feature complement(5269..5325)
/label=MCS
/note="pUC18/19 multiple cloning site"
primer_bind complement(5335..5351)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(5359..5375)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(5383..5413)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(5428..5449)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(5737..6325)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(6499..7356)
/label=AmpR
/note="beta-lactamase"
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