pPIBTcosTET vector (V004106)

LOCUS       40924_34490        7384 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cosmid vector pPIBTcosTET, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7384)
  AUTHORS   Oertel W, Weissgerber A.
  TITLE     Transformation of Ulva mutabilis Foyn with plasmids integrating into
            the chromosome
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7384)
  AUTHORS   Oertel W, Weissgerber A.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-OCT-2007) Biochemistry, Genetics and Microbiology, 
            Division of Genetics, University of Regensburg, Universitaetsstr. 
            31, Regensburg, Bavaria 93053, Germany
REFERENCE   3  (bases 1 to 7384)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7384)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (19-OCT-2007) Biochemistry, Genetics and Microbiology, Division of 
            Genetics, University of Regensburg, Universitaetsstr. 31, 
            Regensburg, Bavaria 93053, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7384
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..546
                     /label=contains Ulva mutabilis RbcS promoter and 5' UTR
                     /note="contains Ulva mutabilis RbcS promoter and 5' UTR"
     intron          555..1051
                     /gene="ble"
                     /note="Ulva mutabilis RbcS intron 1"
     CDS             1050..1415
                     /label=BleoR
                     /note="antibiotic-binding protein"
     3'UTR           1419..1807
                     /label=Ulva mutabilis RbcS 3' UTR
                     /note="Ulva mutabilis RbcS 3' UTR"
     misc_feature    1816..1986
                     /label=cloning vector pUC18, with minor modifications
                     /note="cloning vector pUC18, with minor modifications"
     CDS             complement(3585..4772)
                     /label=TcR
                     /note="tetracycline efflux protein"
     promoter        complement(4820..4848)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     primer_bind     5249..5265
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(5269..5325)
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(5335..5351)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(5359..5375)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5383..5413)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5428..5449)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5737..6325)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6499..7356)
                     /label=AmpR
                     /note="beta-lactamase"