LOCUS       40924_34490        7384 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cosmid vector pPIBTcosTET, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7384)
  AUTHORS   Oertel W, Weissgerber A.
  TITLE     Transformation of Ulva mutabilis Foyn with plasmids integrating into
            the chromosome
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7384)
  AUTHORS   Oertel W, Weissgerber A.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-OCT-2007) Biochemistry, Genetics and Microbiology, 
            Division of Genetics, University of Regensburg, Universitaetsstr. 
            31, Regensburg, Bavaria 93053, Germany
REFERENCE   3  (bases 1 to 7384)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7384)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (19-OCT-2007) Biochemistry, Genetics and Microbiology, Division of 
            Genetics, University of Regensburg, Universitaetsstr. 31, 
            Regensburg, Bavaria 93053, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7384
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..546
                     /label=contains Ulva mutabilis RbcS promoter and 5' UTR
                     /note="contains Ulva mutabilis RbcS promoter and 5' UTR"
     intron          555..1051
                     /gene="ble"
                     /note="Ulva mutabilis RbcS intron 1"
     CDS             1050..1415
                     /label=BleoR
                     /note="antibiotic-binding protein"
     3'UTR           1419..1807
                     /label=Ulva mutabilis RbcS 3' UTR
                     /note="Ulva mutabilis RbcS 3' UTR"
     misc_feature    1816..1986
                     /label=cloning vector pUC18, with minor modifications
                     /note="cloning vector pUC18, with minor modifications"
     CDS             complement(3585..4772)
                     /label=TcR
                     /note="tetracycline efflux protein"
     promoter        complement(4820..4848)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     primer_bind     5249..5265
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(5269..5325)
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(5335..5351)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(5359..5375)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5383..5413)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5428..5449)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5737..6325)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6499..7356)
                     /label=AmpR
                     /note="beta-lactamase"
ORIGIN
        1 cgttgttcgt acacaaggat gtcgcttgtg gctttttcgc tcggctttcc aggagggcag
       61 tccagatacc atgcggccgc cgtgtccatc actcgcgctg cagctgatgt gggcagatac
      121 tgcactaatc tgccgcacga cgcgtcggcc tgagtcggcg gacaccggag gcttgctgga
      181 gtactggcac aagtgtgcgt gtgattgcca gaaacagcaa cccactcact agatataccg
      241 ctccaataat gggttcatgt gcaagctcac tcaataacga aacgcttcac tattgtgcac
      301 gtgccggggt tgcttgggta tggcgtgcca tacgggttct gcacatgtca cccacgtggg
      361 tgtggcacga gatatcgagg tggccatgta aaaggcattt gctagagggc agctcgtcca
      421 tgccgctcga gcacaccgag tgcgcaccat aaatgcagca catgcctgtg cagcctatct
      481 cctgtctctg ttacatccca ccacctcctc acatatcccc ttccaccctt aaaccgcaca
      541 gcagacatgg ccaagtgcgc atcgcgtgtc aatgctggcg ctgctgacgt ccgctgccta
      601 aactgcatga aagctcctgc agtcgcaagt agtcctttta acttgttgta tcggaacaaa
      661 tggttgtagt ctgtgccgtg tgacgatctt gagccgcctg gtcggcaggt ccaatgtcgg
      721 cctggaccct gcacaccctg cccaccctgc cgctgctcac ccgttcacga ccccacaggt
      781 gctcgcagca gtaattgtcg atagatagca actgtgttct gactattctt tgaccttccg
      841 catttgggac tgcggcggca cacctactat ccgcaatgca caccctggtg ctggtactgc
      901 ctgcctttcc gcgcgagccc acaccatgca ggcgcatagt aatggactgt gattgattta
      961 ggcaactgta tgaaacctct tcatcgtgcc ttggttgctt gcgataccct cacgtgagtt
     1021 catatgacca acgctggcac gacccctgca ggatcaccag cgccgttccg gtgctcaccg
     1081 cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg gctcgggttc tcccgggact
     1141 tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg
     1201 tccaggacca ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg
     1261 agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg
     1321 ccatgaccga gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc gacccggccg
     1381 gcaactgcgt gcacttcgtg gccgaggagc aggactaagc agtcaggcag agtcgttgtt
     1441 gggtgtatgg ttgcacaaag ctagacctcg agctggtcgg ttagaaagag atgcttgtgc
     1501 gactggatgt aacaggcaca atccgaggaa aatgcaacag aaacgttatg atgtaacatc
     1561 tgagcacagt caataaaatt gcatgttgta tgccgcacac aaaagtgttg ttaaacatgc
     1621 gatgtggttg tcaaatcatt attcgcacaa ccatggaccg aacctctggc acgctgcata
     1681 cgctgtattc attcacatca tgttttctac ttgtcacatg ctctgcacca agtaactccg
     1741 attagttagg ttcgcacatg tcatcggagt ggaccacttc gaatcatttg gttcgaagac
     1801 aaaccggggg aattattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     1861 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     1921 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
     1981 cacgaggcca attcggatct gcctgctggc ctgccgcagt tcttcaacct cccggcgcag
     2041 cttttcgttc tcaatttcag catccctttc ggcataccat tttatgacgg cggcagagtc
     2101 ataaagcacc tcattaccct tgccaccgcc tcgcagaacg ggcattccct gttcctgcca
     2161 gttctgaatg gtacggatac tcgcaccgaa aatgtcagcc actgcttttt gttgacttcc
     2221 attgttcatt ccacggacaa aaacagagaa aggaaacgac agaggccaaa aagctcgctt
     2281 tcagcacctg tcgtttcctt tcttttcaga gggtatttta aataaaaaca ttaagttatg
     2341 acgaagaaga acggaaacgc cttaaaccgg aaaattttca taaatagcga aaacccgcga
     2401 ggtcgccgcc ccgtaacaag gcggatcgcc ggaaaggacc cgcaaatgat aataattatc
     2461 aattgcatac tatcgacggc actgctgcca gataacacca ccggggaaac attccatcat
     2521 gatggccgtg cggacatagg aagccagttc atccatcgct ttcttgtctg ctgccatttg
     2581 ctttgtgaca tccagcgccg cacattcagc agcgtttttc agcgcgtttt cgatcaacgt
     2641 ttcaatgttg gtatcaacac caggtttaac tttgaactta tcggcactga cggttacaat
     2701 ttccggatct gcctcgctgg cctgccgcag ttcttcaacc tcccggcgca gcttttcgtt
     2761 ctcaatttca gcatcccttt cggcatacca ttttatgacg gcggcagagt cataaagcac
     2821 ctcattaccc ttgccaccgc ctcgcaaaac gggcattccc tgttcctgcc agttctgaat
     2881 ggtacggata ctcgcaccga aaatgtcagc cagctaggga taacagggta atctgctttt
     2941 tgttgacttc cattgttcat tccacggaca aaaacagaga aaggaaacga cagaggccaa
     3001 aaagctcgct ttcagcacct gtcgtttcct ttcttttcag agggtatttt aaataaaaac
     3061 attaagttat gacgaagaag aacggaaacg ccttaaaccg gaaaattttc ataaatagcg
     3121 aaaacccgcg aggtcgccgc cccgtaacaa ggcggatcgc cggaaaggac ccgcaaatga
     3181 taataattat caattgcata ctatcgacgg cactgctgcc agataacacc accggggaaa
     3241 cattccatca tgatggccgt gcggacatag gaagccagtt catccatcgc tttcttgtct
     3301 gctgccattt gctttgtgac atccagcgcc gcacattcag cagcgttttt cagcgcgttt
     3361 tcgatcaacg tttcaatgtt ggtatcaaca ccaggtttaa ctttgaactt atcggcactg
     3421 acggttaccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt
     3481 ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct
     3541 tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg
     3601 ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc
     3661 catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt
     3721 ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg
     3781 tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa
     3841 gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg
     3901 tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg
     3961 gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc
     4021 gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc
     4081 gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg
     4141 atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc
     4201 ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg
     4261 ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc
     4321 ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg
     4381 cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg
     4441 gcgccggtga tgccggccac gatgcgtccg gcgtagagga tccacaggac gggtgtggtc
     4501 gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg
     4561 ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat
     4621 agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag
     4681 aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg
     4741 aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt
     4801 aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattg
     4861 ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
     4921 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
     4981 tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta
     5041 ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
     5101 atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc
     5161 tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta
     5221 acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttgcatgc
     5281 ctgcaggtcg actctagagg atccccgggt accgagctcg aattcgtaat catggtcata
     5341 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     5401 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     5461 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     5521 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     5581 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     5641 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     5701 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     5761 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     5821 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     5881 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     5941 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     6001 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     6061 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     6121 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
     6181 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     6241 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     6301 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     6361 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     6421 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     6481 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     6541 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     6601 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     6661 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     6721 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     6781 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     6841 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     6901 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     6961 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     7021 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     7081 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     7141 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     7201 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     7261 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     7321 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aataattccc
     7381 atgg
//
