Basic Vector Information
- Vector Name:
- pPIBTcosCAT
- Antibiotic Resistance:
- Ampicillin
- Length:
- 7436 bp
- Type:
- Cosmid vector
- Replication origin:
- ori
- Source/Author:
- Oertel W, Weissgerber A.
pPIBTcosCAT vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pPIBTcosCAT vector Sequence
LOCUS 40924_34485 7436 bp DNA circular SYN 18-DEC-2018 DEFINITION Cosmid vector pPIBTcosCAT, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7436) AUTHORS Oertel W, Weissgerber A. TITLE Transformation of Ulva mutabilis Foyn with plasmids integrating into the chromosome JOURNAL Unpublished REFERENCE 2 (bases 1 to 7436) AUTHORS Oertel W, Weissgerber A. TITLE Direct Submission JOURNAL Submitted (19-OCT-2007) Biochemistry, Genetics and Microbiology, Division of Genetics, University of Regensburg, Universitaetsstr. 31, Regensburg, Bavaria 93053, Germany REFERENCE 3 (bases 1 to 7436) TITLE Direct Submission REFERENCE 4 (bases 1 to 7436) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Unpublished" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (19-OCT-2007) Biochemistry, Genetics and Microbiology, Division of Genetics, University of Regensburg, Universitaetsstr. 31, Regensburg, Bavaria 93053, Germany" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..7436 /mol_type="other DNA" /organism="synthetic DNA construct" misc_feature 1..546 /label=contains Ulva mutabilis RbcS promoter and 5' UTR /note="contains Ulva mutabilis RbcS promoter and 5' UTR" intron 555..1051 /gene="ble" /note="Ulva mutabilis RbcS intron 1" CDS 1050..1415 /label=BleoR /note="antibiotic-binding protein" 3'UTR 1419..1807 /label=Ulva mutabilis RbcS 3' UTR /note="Ulva mutabilis RbcS 3' UTR" misc_feature 1816..1986 /label=cloning vector pUC18, with minor modifications /note="cloning vector pUC18, with minor modifications" CDS complement(4031..4687) /label=CmR /note="chloramphenicol acetyltransferase" promoter complement(4688..4790) /label=cat promoter /note="promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase" primer_bind 5301..5317 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" misc_feature complement(5321..5377) /label=MCS /note="pUC18/19 multiple cloning site" primer_bind complement(5387..5403) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind complement(5411..5427) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(5435..5465) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(5480..5501) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(5789..6377) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(6551..7408) /label=AmpR /note="beta-lactamase"
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